Armut çeşitlerinin SSR markörlerine göre genetik çeşitliliğinin belirlenmesi ve Armut memeli pasına (Gymnosporangium fuscum) reaksiyonları

Year 2020, Volume: 60 Issue: 3, 15 - 24, 30.09.2020

Suat Kaymak , Hasan Pinar

https://doi.org/10.16955/bitkorb.597886

Abstract

Armut, Türkiye ve dünyanın farklı ekolojik koşullarında yaygın olarak yetiştirilen bir bitki türüdür. Armut yetiştiriciliği Türkiye'de uzun bir geçmişe sahiptir, ancak bu eşsiz genetik kaynakların tarımsal ekosistemdeki ve genetik erozyondaki değişimler nedeniyle nesli tükenme tehlikesi bulunmaktadır. Gymnosporangium fuscum'un neden olduğu armut memeli pas hastalığı, armut ağaçlarının önemli hastalıklarından biridir ve aynı zamanda ardıç ağaçlarında da bulunur (Juniperus oxycedrus L. ve J. excelsa Bieb). Ardıç popülasyonlarının yakınındaki bazı armut bahçelerinde ciddi ekonomik kayıplar görülmüştür. Bu çalışmada, Çöğür ve Quince A (QA) üzerine aşılanmış 25 armut çeşidinin genetik çeşitliliği 13 SSR primer kullanılarak belirlenmiştir ve bu armut çeşitlerinin armut memeli pası (Gymnosporangium fuscum)’na karşı reaksiyon seviyeleri incelenmiştir. 25 ticari açıdan önemli armut çeşidi iki ana grup içinde yakın ilişkili olarak ele alınmıştır. Bu SSR markörleri armut genetik çeşitliliğin tanımlanmasında faydalı ve güvenilir olarak kullanılabileceğini göstermiştir. Hastalık şiddeti oranlarına göre yıllar ve anaçlar arasındaki fark istatistiki yönden önemli bulunmuştur. Hastalık tüm armut çeşitlerinde görülmüş ve hiçbir armut çeşidi hastalığa karşı dayanıklı olarak değerlendirilmemiştir. QA anaçlı armut çeşitlerinde çöğür anacına göre hastalık şiddeti daha yüksek tespit edilmiştir.

Keywords

armut, genetik çeşitlilik, SSR, armut memeli pası

Genetic diversity of pear cultivars using SSR markers and their reactions to pear rust (Gymnosporangium fuscum)

Abstract

Commonly grown in different ecological conditions of Turkey and the world, pear (Pyrus communis L.), as a plant species, has a long cultivation history in Turkey. However, its unique genetic resources are in danger of extinction due to changes in the agro-ecosystem and genetic erosion. In addition, pear rust caused by Gymnosporangium fuscum is one of the significant diseases of pear. Severe economic losses have been reported in some pear orchards near the extensive juniper populations. In the present study, the genetic diversity and sensitivity level to pear rust (Gymnosporangium fuscum) of 25 local and commercially important pear varieties grafted on seedling and Quince A (QA) was determined using 13 SSR primers. The selected verities were clustered into two major groups that were closely related. The SSR markers provided reliable genotyping and demonstrated their usefulness for identifying pear genetic diversity. The difference between years and rootstocks according to disease severity rates was found to be statistically significant. Although none of the pear varieties assessed in these experiments were resistant to rust, the disease severity of the pear varieties of QA rootstock was generally higher than that of the seedling varieties.

Keywords

pear, genetic varieties, SSR, pear rust

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