Bemisia tabaci Gennadius (Hemiptera: Aleyrodidae) popülasyonlarında nikotinik asetilkolin reseptör genlerinin karşılaştırılması

Year 2020, Volume: 60 Issue: 3, 73 - 80, 30.09.2020

Gül Satar Mehmet Rifat Ulusoy

https://doi.org/10.16955/bitkorb.704856

Abstract

Nikotinik asetilkolin reseptörleri (nAChRs) böcek sinir sisteminde hızlı kolinerjik sinaptik taşınmada görevlidirler. Neonikotinoid grubu insektisitler de bu reseptörleri hedef alarak böceklerin ölmesine neden olurlar. Bu çalışmada, Doğu Akdeniz Bölgesi’nden toplanan bazı Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) popülasyonlarında nAChR α1, α3 ve α4 genleri karşılaştırılarak filogenetik ilişkiler ortaya konulmuştur. Denemede Karataş-Gossypium hirsutum (Adana), Aydıncık-Solanum lycopersicum, Erdemli-S. lycopersicum, Samandağ-Cucumis sativus (Hatay) ve Kumluca-Capsicum annum (Antalya)’dan toplanan beş örnekle çalışmalar yürütülmüştür. nAChR α1, 3 ve 4 genleri tüm popülasyonlar için karşılaştırıldığında α1 genleri arasında bir farklılık belirlenmezken; α3 geninde referans gene (full α3) göre tüm popülasyonlarda V147I, A227T ve T534I aminoasitlerinde ve α4 geninde Aydıncık’ta S401G ve referans gene (full α4) göre tüm popülasyonlarda G198E spesifik aminoasit değişimleri belirlenmiştir. NCBI’dan seçilen aynı gen bölgeleriyle yapılan filogenetik analizlerde bu çalışmada çalışılan her gen kendi gen ailesi içerisinde yer almıştır. Elde edilen bu verilerin neonikotinoid grubu insektisitler nokta mutasyona dayalı dirençle ilgili ileride yürütülebilecek çalışmalara ışık tutması amaçlanmıştır.

Keywords

Beyazsinek, filogenetik analiz, nAChR, neonikotinoid

Supporting Institution

Çukurova Üniversitesi BAP

Project Number

D3BAP2012

Thanks

Bu çalışma doktora tez çalışmasının sonuçlarını içermekte olup, desteklerinden dolayı Çukurova Üniversitesi BAP birimine (D3BAP2012) teşekkür ederiz. Ayrıca Bemisa tabaci nAChR α1, α3 ve α4 genlerine ait gen ve primer bilgisini bizimle paylaşan Dr. Martin Wlliamson (Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, United Kingdom)’a sonsuz şükranlarımızı sunarız.

Comparison of nicotinic acetylcholine receptor genes in Bemisia tabaci Gennadius (Hemiptera: Aleyrodidae) populations

Abstract

Nicotinic acetylcholine receptors (nAChRs) mediate fast cholinergic synaptic transmission in the insect nervous system. Neonicotinoid group insecticides target these receptors, causing mortality at the insects. Phylogenetic relationship was revealed comparing nAChR α1, α3, and α4 genes for the some Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) populations belonging to East Mediterranean Region. Five B. tabaci populations collected from Karataş-Gossypium hirsutum (Adana), Aydıncık-Solanum lycopersicum, Erdemli-S. lycopersicum, (Mersin), Samandag-Cucumis sativus (Hatay), and Kumluca-Capsicum annum (Antalya) were used for the experiment. When nAChR α1, 3, and 4 genes were compared for all populations, no differences were determined for the α1 gene. However, V147I, A227T, and T534I unique amino acid changes in all populations according to the reference gene (full α3) in the α3 gene, S401G in Aydıncık, and G198E in all populations according to a reference gene (full α4) were determined. Phylogenetic tree results indicate that each novel isolates were clustered its own gene group of represensative isolates deposited from NCBI. The obtained results could shed light on the projects that can be carried out in the future regarding the point mutation-based resistance to the neonicotinoid group insecticides.

Keywords

nAChR, neonicotinoid, phylogenetic analyses, whitefly

Project Number

D3BAP2012

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