Türkiye'den Cryptocephalus turcicus Suffrian, 1847'nin (Coleoptera: Chrysomelidae: Cryptocephalinae) aedeagus ve spermatheca ince yapısının SEM kullanılarak araştırılması

Year 2022, Volume: 62 Issue: 4, 22 - 26, 31.12.2022

Neslihan Bal , Huseyin Özdikmen , Damla Amutkan Mutlu , Zekiye Suludere , Didem Coral

https://doi.org/10.16955/bitkorb.1163021

Abstract

Makale, Türkiye'den bir bitki zararlısının (Coleoptera: Chrysomelidae: Cryptocephalinae) Cryptocephalus turcicus Suffrian, 1847'de aedeagus ve spermatecanın SEM tarafından ilk kez gözlemlenen ultrastrüktürlerini sunmaktadır. Bu çalışmada, Cryptocephalus (s.str.) nominatif alt cinsine ait bu türün Ankara, Kastamonu ve Samsun illerinden toplanan türün erkek ve dişi üreme organlarının morfolojileri Taramalı Elektron Mikroskobu (Scanning Electron Microscopy-SEM) kullanılarak incelenmiştir. Bugüne kadar Türkiye'de Cryptocephalus turcicus Suffrian, 1847'nin erkek genital (aedeagus) ve dişi genital (spermatheca) morfolojilerinin birlikte çalışıldığı bu kapsamda bir çalışmaya rastlanmamıştır. Cryptocephalus cinsi Türkiye'de 73 tür ile temsil edilmektedir. Aedeagus ve spermatecanın detaylı araştırılması Cryptocephalus cinsinde yeni tanısal karakterler elde etmek için çok önemlidir. Bu nedenle, Cryptocephalus turcicus Suffrian, 1847'nin Türkiye'den aedeagus ve spermatecalarının ultrastrüktürel ve detaylı incelemeleri ilk olarak Cryptocephalus cinsinde yeni tanısal karakterler elde etmek için SEM ve stereo mikroskop ile incelenmiştir. Türlere ait aedeagus ve spermatecanın ultrastrüktürünün incelenmesiyle elde edilen yeni tanısal karakterler, Cryptocephalus cinsindeki türler arasındaki farklar ve önemli karakterler belirlenerek özellikle birbirine benzeyen türlerin ayrımında kullanılacaktır. SEM'deki fotoğraflar ve stereo mikroskoptaki fotoğraflar da metinde verilmiştir.

Keywords

Cryptocephalus turcicus, aedeagus, spermatheca, SEM, Türkiye, Cryptocephalus turcicus, aedeagus, spermatheca, SEM, Türkiye

Investigation of aedeagus and spermatheca ultrastructure of Cryptocephalus turcicus Suffrian, 1847 (Coleoptera: Chrysomelidae: Cryptocephalinae) from Türkiye by using SEM

Abstract

This paper presents ultrastructures of aedeagus and spermatheca observed for the first time by SEM of a plant pest (Coleoptera: Chrysomelidae: Cryptocephalinae) Cryptocephalus turcicus Suffrian, 1847 from Türkiye. In the present study, the morphologies of male and female genitalia of this species belonging to the nominative subgenus Cryptocephalus (s.str.) collected from Ankara, Kastamonu and Samsun provinces are examined using Scanning Electron Microscopy (SEM). To date, there has not been a study in Türkiye in this context in which male genitalia (aedeagus) and female genitalia (spermatheca) morphologies of the Cryptocephalus turcicus Suffrian, 1847 have been studied together. The genus Cryptocephalus is represented by 73 species in Türkiye. Detailed investigations of aedeagus “and spermatheca are very important to obtain new diagnostic characters in the genus Cryptocephalus. For this reason, ultrastructural and detailed investigations of aedeagus and spermatheca of Cryptocephalus turcicus Suffrian, 1847 from Türkiye were firstly studied with SEM and stereo microscope to obtain new diagnostic characters in the genus Cryptocephalus. The new diagnostic characters obtained by examining the ultrastructure of aedeagus and spermatheca belonging to the species will be used in the distinction of species that are especially similar to each other, by determining the differences and important characters among the species in the genus Cryptocephalus. Photos in SEM, as well as photos in the stereo microscope, are also given in the text.

Keywords

Cryptocephalus turcicus, aedeagus, spermatheca, SEM, Türkiye

References

• Basset Y., Samuelson G.A., 1996. Ecological characteristics of an arboreal community of Chrysomelidae in Papua New Guinea. In: Jolivet PHA, Cox ML (Eds) Chrysomelidae Biology Volume 2: Ecological Studies. SPB Academic Publishing, Amsterdam, 243–262.
• Chamorro-Lacayo M. L., 2014. 2.7.5 Chrysomelidae: CryptocephalinaeGyllenhal 1813. In: Leschen RAB, Beutel RG (Eds) Handbook of Zoology. Arthropoda: Insecta: Coleoptera Volume 3: Morphology and Systematics (Phytophaga). De Gruyter, USA, 81–87.
• Flowers R. W., 1996. La subfamilia Eumolpinae (Coleoptera: Chrysomelidae) en América Central. Publicación Especial de la Revista de Biología Tropical 2: 1–60
• Gómez-Zurita J., Hunt T., Kopliku F., Vogler A.P., 2007. Recalibrated tree of leaf beetles (Chrysomelidae) indicates independent diversification of angiosperms and their insect herbivores. PLoS ONE 2: e360. https://doi.org/10.1371/journal.pone.0000360.
• González-Megías A., Gómez J. M., 2003. Consequences of removing a keystone herbivore for the abundance and diversity of arthropods associated with a cruciferous shrub. Ecological Entomology 28: 299–308. https://doi.org/10.1046/j.1365-2311.2003.00510.x
• Jolivet P., 1988 Food habits and food selection of Chrysomelidae. Bionomics and evolutionary perspectives. In: Jolivet P, Petitpierre E, Hsiao TH (eds) Biology of Chrysomelidae. Kluwer, Dordrecht. pp 1–24.
• Jolivet P., Verma K. K., 2008. Eumolpinae – a widely distributed and much diversified subfamily of leaf beetles (Coleoptera, Chrysomelidae). Terrestrial Arthropod Reviews 1: 3–37. https://doi.org/10.1163/187498308X345424
• Lopatin I. K., Dovgailo K. E., 2002. The genus Cryptocephalus (Chrysomelidae) of Palearctic region. CD key and database on the basis of software “Lysandra”. Minsk.
• Matsuda K., 1988. Feeding stimulants of leaf beetles. In: Jolivet P, Petitpierre E, Hsiao TH (eds) Biology of Chrysomelidae. Kluwer, Dordrecht, pp 41–56.
• Özdikmen, H. and Cihan, N., 2014. Chorotype identification for Turkish Chrysomeloidea (Coleoptera) Part III–Chrysomelidae: Cryptocephalinae. Munis Entomology & Zoology, 9(1), 125-142. Riley E. G., Clark S. M., Flowers R. W., Gilbert A. J., 2002. 124 Chrysomelidae Latreille 1802. In: Arnett RH, Thomas MC, Skelley PE, Frank JH (Eds) Volume 2, American Beetles. Polyphaga: Scarabaeoidea through Curculionoidea. CRC Press LLC, USA, 617–691.
• Schöller, M., 2008. Comparative morphology of sclerites used by Camptosomatan leaf beetles for formation of the extrachorion (Chrysomelidae: Cryptocephalinae, Lamprosomatinae). In: Jolivet, P., Santiago-Blay, J. & Schmitt, M. (Eds.), Research on Chrysomelidae. Vol. 1. Brill, Leiden, pp. 87–120.
• Staines C. L., 2002. The New World tribes and genera of hispines (Coleoptera: Chrysomelidae: Cassidinae). Proceedings of the Entomological Society of Washington 104(3): 721–784.
• Strauss Y. S., 1988. The Chrysomelidae: a useful group for investigating herbivore–herbivore interactions. In: Jolivet P, Petitpierre E, Hsiao TH (eds) Biology of Chrysomelidae. Kluwer, Dordrecht, pp 91–105.
• Warchalowski A., 2003. Chrysomelidae. The Leaf-Beetles of Europe and the Mediterranean Area. Natura Optima Dux Foundation, Warszawa, 600 pp.
• White R. E., 1968. A review of the genus Cryptocephalus in America north of Mexico (Chrysomelidae: Coleoptera). United States National Museum Bulletin 290: 1–124. https://doi. org/10.5479/si.03629236.290.1
• Wilcox J. A., 1972. A review of the North American Chrysomelinae leaf beetles (Coleoptera: Chrysomelidae). Bulletin 421. University of the State of New York, State Education Department, State Museum and Science Service, 37 pp.