Anastomosis grouping and phylogenetic analysis of Rhizoctonia isolates on wheat in Türkiye

Year 2023, Volume: 63 Issue: 2, 5 - 15, 30.06.2023

Filiz Ünal , Sara Dolar

https://doi.org/10.16955/bitkorb.1263982

Abstract

This study aims to determine the species and evaluate the genetic diversity of the pathogenic and nonpathogenic Rhizoctonia spp. and anastomosis groups (AG) from wheat plants and rhizosphere soils in Turkey. Rhizoctonia species were isolated from plants and rhizosphere soils in wheat fields in 5 provinces in the Central Anatolian Region of Türkiye. As a result of the isolations, a total of 88 multinucleate (MN) and binucleate (BN) Rhizoctonia isolates were obtained. Identifications of the isolates were determined by rDNA-ITS sequence analyses. The identified isolates belonged to MN Waitea circinata var. zeae, W. circinata var. oryzae, W. circinata var. circinata, MN Rhizoctonia solani AG 2-1, AG 2-2, AG 3, AG 4-HGII, AG 4-HGIII, AG 5, AG 8, AG 11 and BN AG A, AG DI, AG E, AG G, AG H, AG I, AG I-like and AG K. The most isolated group was W. circinata var. circinata. In the pathogenicity studies, the most virulent group was determined as R. solani AG 4. Among the binucleate isolates, groups other than R. cerealis AG DI were not found to be pathogenic. Neighbor-joining phylogenetic trees of isolates were constructed from rDNA-ITS sequences. As a result of this study, the regional distribution of MN and BN Rhizoctonia AG isolates in important wheat production areas in the Central Anatolia Region, Türkiye was determined. In addition, this study is the first comprehensive study in which the genetic diversity of Rhizoctonia AGs isolates obtained from wheat and rhizosphere soils in the region was evaluated with a molecular approach.

Keywords

anastomosis groups, Rhizoctonia, neighbour-joining, soil, wheat

Supporting Institution

Technological Research Council of Turkey (TUBITAK)

Project Number

Project No: TOVAG-110O622

Thanks

We are grateful to Dr. Erkol Demirci (Türkiye), Dr. Francesca Cardinale (Italy), Dr. Takeshi Toda (Japan), Dr. Grzegorz Lemańczyk (Poland) and Dr. Patricia Okubara (USA), who provided us Rhizoctonia tester isolates and the Scientific and Technological Research Council of Turkey (TUBITAK) for a grant (Project No: TOVAG-110O622).

Anastomosis grouping and phylogenetic analysis of Rhizoctonia isolates on wheat in Türkiye

Abstract

Bu çalışmanın amacı, Türkiye'deki buğday bitkileri ve rizosfer topraklarından patojenik ve patojenik olmayan Rhizoctonia tür ve anastomosis gruplarının (AG) türlerini belirlemek ve genetik çeşitliliklerini değerlendirmektir. Türkiye'nin Orta Anadolu Bölgesi'ndeki 5 ildeki buğday tarlalarının bitki ve rizosfer topraklarından Rhizoctonia türleri izole edilmiştir. İzolasyonlar sonucunda, toplam 88 adet çok çekirdekli (MN) ve iki çekirdekli (BN) Rhizoctonia izolatı elde edilmiştir. İzolatların teşhislerinde rDNA-ITS dizi analizi yöntemi kullanılmıştır ve MN Waitea circinata var. zeae, W. circinata var. oryzae, W. circinata var. circinata, MN Rhizoctonia solani AG 2-1, AG 2-2, AG 3, AG 4-HGII, AG 4-HGIII, AG 5, AG 8, AG 11 ve BN AG A, AG DI, AG E, AG G, AG H, AG I, AG I-benzeri ve AG K'ya ait oldukları belirlenmiştir. En çok izole edilen grup W. circinata var. circinata olmuştur. Patojenite çalışmalarında, en virulent grubun R. solani AG 4 olduğu saptanmıştır. BN izolatlar arasında, R. cerealis AG DI dışındaki diğer grupların patojen olmadığı tespit edilmiştir. İzolatların rDNA-ITS dizilerinden neighbor-joining filogenetik ağaçları oluşturulmuştur. Bu çalışmanın sonucunda, Türkiye’nin Orta Anadolu Bölgesi'ndeki önemli buğday üretim alanlarında MN ve BN Rhizoctonia AG izolatlarının bölgedeki dağılımı belirlenmiştir. Ayrıca, bu çalışma, bölgeden elde edilen Rhizoctonia AG izolatlarının genetik çeşitliliğinin moleküler bir yaklaşımla değerlendirildiği ilk kapsamlı çalışmadır.

Keywords

akrabalık ilişkileri, anastomosis grup, buğday, Rhizoctonia, toprak

Project Number

Project No: TOVAG-110O622

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