Nematophagous fungi species from Erzurum and Erzincan provinces in Türkiye

Year 2024, Volume: 64 Issue: 3, 13 - 19, 01.10.2024

Tuba Genç Kesimci , Erkol Demirci

https://doi.org/10.16955/bitkorb.1385592

Abstract

In Erzurum and Erzincan provinces, some nematophagous fungi were determined on roots of alfalfa (Medicago sativa L.), potato (Solanum tuberosum L.), and strawberry (Fragaria x ananassa Duchesne) plants during 2009-2011, and identification of fungi species was performed by classical and/or molecular techniques. Purified 5 isolates were determined as Arthrobotrys cladodes Drechsler 1937 (1 isolate), Arthrobotrys conoides Drechsler 1937 (1 isolate) and Arthrobotrys superba Corda 1839 (3 isolates). Identification of Arthrobotrys isolates was also confirmed by ribosomal DNA (rDNA)-ITS (internal transcribed spacer) sequence analysis. Harposporium genus was also determined considering the morphological characteristic of fungal spores growing on nematodes found in two samples, but these fungi could not be purified. Harposporium species were identified as Harposporium anguillulae Lohde emend. Zopf 1888 and Harposporium crassum A.M. Sheph 1955 according to the morphological characteristic. To our knowledge, all Arthrobotrys and Harposporium species identified in this study are reported for the first time in Türkiye.

Keywords

Arthrobotrys, Harposporium, nematophagous fungi, rDNA-ITS region.

Türkiye'de Erzurum ve Erzincan illerinden elde edilen nematofag fungus türleri

Abstract

Erzurum ve Erzincan illerinde yonca (Medicago sativa L.), patates (Solanum tuberosum L.) ve çilek (Fragaria x ananassa Duchesne) bitkilerinin köklerinde 2009-2011 yıllarında bazı nematofag funguslar belirlenmiş ve fungus türlerinin tanılanması klasik ve/veya moleküler tekniklerle gerçekleştirilmiştir. Saflaştırılan 5 izolatın Arthrobotrys cladodes Drechsler 1937 (1 izolat), Arthrobotrys conoides Drechsler 1937 (1 izolat) ve Arthrobotrys superba Corda 1839 (3 izolat) türlerine ait olduğu belirlenmiştir. Arthrobotrys izolatlarının tanılanması ribozomal DNA (rDNA)-ITS (internal transcribed spacer) baz dizi analizi ile de doğrulanmıştır. İki örnekte bulunan nematodlar üzerinde gelişen sporların morfolojik özellikleri dikkate alınarak Harposporium cinsi de belirlenmiş, ancak bu funguslar saflaştırılamamıştır. Harposporium türleri morfolojik özelliklerine göre Harposporium anguillulae Lohde emend. Zopf 1888 ve Harposporium crassum A.M. Sheph 1955 olarak tanılanmıştır. Bildiğimiz kadarıyla bu çalışmada belirlenen tüm Arthrobotrys ve Harposporium türleri Türkiye’de ilk defa rapor edilmiştir.

Keywords

Arthrobotrys, Harposporium, nematofag fungus, rDNA-ITS bölgesi

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