Isolation and Characterization of Alkane Hydrocarbons-Degrading Enterobacter ludwigii strain D8 from Petroleum-Contaminated Soils

Year 2022, , 630 - 639, 30.06.2022

Ayşe Eren , Kemal Güven

https://doi.org/10.17798/bitlisfen.1071785

Abstract

A bacterial strain has been isolated from petroleum contaminated soil with in southeastern Turkey. This isolated strain was characterized to determine its hydrocarbon biodegradation potential. Phenotypic features and of 16 S gene sequence analysis of rRNA revealed that strain D8 belongs to the Enterobacter genus and most closely resembles Enterobacter ludwigii (100%). The optimum temperature and pH values for the growth of E. ludwigii D8 were found to be 30°C and 5.0, respectively. This bacterial strain grew in long and medium chain hydrocarbons such as 1% decane, pentadecane and squalene separately at the end of 3 day incubation in the basal medium (BM) under optimum conditions. It was shown that E. ludwigii strain D8 degrades about 27% of crude oil incubated for 5 days, while it degrades 29% of pentadecane after 3 days of incubation determined by Gas chromatography-MS analysis. The biodegradation potential of petroleum hydrocarbons of E. ludwigii strain D8 isolated and characterized in this study indicates that this strain may play a role in the bioremediation process.

Keywords

Oil pollution, bacterial characterization, 16S rRNA gene sequence analysis, GC-MS analysis, Bioremediation

Supporting Institution

Dicle University

Project Number

19.014

Alkan Hidrokarbonları Parçalayan Enterobacter ludwigii strain D8 Suşunun Petrolle Kirlenmiş Topraklardan İzolasyonu ve Karakterizasyonu

Abstract

Türkiye'nin güneydoğusundaki petrol bulaşmış topraktan bir bakteri türü izole edildi. Bu izole edilen bakteri, petrol hidrokarbonları açısından biyolojik bozunma potansiyelini belirlemek için karakterize edildi. Fenotipik özellikler ve 16 S rRNA geninin dizi analizi, suş D8'in Enterobacter cinsinin bir üyesi olduğunu ve en çok Enterobacter ludwigii ‘ ye (%100) benzediğini belirlendi. E. ludwigii suşu D8'in büyümesi için optimum sıcaklık ve pH değerleri sırasıyla 30°C ve 5.0 olarak bulunmuştur. Optimum koşullarda bazal ortamda (BM) uzun ve orta zincirli hidrokarbon olan %1’lik dekan, pentadekan ve skualen ile ayrı ayrı inkübasyon sonunda bu bakteri suşunun ürediği görülmüştür. E. ludwigii D8 suşunun, yapılan GC-MS analizlerinde, 5 günlük inkübasyon sonrasında ham petrolü yaklaşık % 27 oranında parçalarken, 3 günlük inkübasyonu sonrasında pentadekanı % 29 oranında parçaladığı belirlendi. Bu çalışmada, E. ludwigii strain D8 suşunun, petrol hidrokarbonlarını biyolojik olarak parçalama özelliğinden dolayı, biyoiyileştirme sürecinde rol alabileceğini göstermektedir.

Keywords

Petrol kirliliği, bakteriyel karakterizasyon, 16S rRNA gen dizisi analizi, GC-MS analizi, Biyoiyileştirme

Project Number

19.014

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