The Antioxidant Capacities and Antimicrobial Activities of Some Salvia L. Seeds

Year 2023, , 994 - 1005, 28.12.2023

Murat Kürşat , Sevda Kırbağ , İrfan Emre , Pınar Erecevit Sönmez , Mustafa Yunus Emre , Prof. Dr. Ökkeş Yılmaz , Şemsettin Civelek

https://doi.org/10.17798/bitlisfen.1312188

Abstract

The goal of present study is to find the penolic contents, antioxidant activities and antimicrobial capacities in the seeds of five Salvia L. taxa two of which are endemics (Salvia euphratica var. leiocalycina and Salvia euphratica var. euphratica). The flavonoid and phenolic acid are determined by using HPLC while the antioxidant activities are determined based on different methods. Also, the antimicrobial activities of some Salvia species are determined by using the well agar method. The current study found that the studied Salvia species have low flavonoid. It has been found that Salvia euphratica var. euphratica has high vanillic acid, ferulic acid and rosmarinic acid among the studied taxa. Similarly, it has been found that Salvia euphratica var. euphratica has high DPPH and ABTS radical scavening capacity in all concentrations. It has been also found that Salvia euphratica var. euphratica has highest total phenolic content (372,63±0,87 µgGAE/mg) whilst Salvia tricholoda has low total phenolic content (46,41±1,71 µgGAE/mg). In addition, this study demonstrated that Salvia tricholoda has lowest metal chelating activity (37,35±0,51%). Furthermore, present study found that the lipid peroxidation levels of the studied Salvia taxa are between 18,21±0,37 mg/kg and 21,03±0,22 mg/kg while it has been found that the antibacterial properties of the Salvia taxa under study are altering.

Keywords

Antimicrobial Activity, Antioxidant Capacity, Phenolics, Salvia L

Supporting Institution

Fırat University

Project Number

Project Number: FUBAP 2041.

Thanks

The Firat University Scientific Research Unit is funding this study (Project Number: FUBAP 2041). We gratefully acknowledge the Scientific Research Unit of Firat University. Additionally, certain study data are presented orally, and an abstract of the study is included in the National Botanical Science Congress Abstract Book.

References

• [1] A. Ulubelen, H. Birman, S. Oksuz, G. Topcu, U. Kolak, A. Barla, and W. Voelter, "Cardioactive diterpenes from the roots of Salvia eriophora," Planta Medica, vol. 68, no. 9, pp.818-821, 2002.
• [2] E. Kupeli-Akkol, F. Goger, M. Kosar, and K. H. C. Baser, "Phenolic composition and biological activities of Salvia halophila and Salvia virgate, "Food Chemistry, vol.108, pp.942-949, 2008.
• [3] M. B. Bahadori, B. Asghari, L. Dinparast, G. Zengin, C. Sarikurkcu, M. Abbas-Mohammadi, and S. Bahador, "Salvia nemorosa L. A novel source of bioactive agents with functional connections," Lwt-Food Science annd Technology, vol.75, pp.42-50, 2017.
• [4] K. Aktas, C. Ozdemir, M. Ozkan, and P. Baran, "Pollen morphology of some Turkish Salvia L. (Lamiaceae: Mentheae) species," Planta Daninha, vol. 38, pp. 1-12, 2020.
• [5] H. Maral, "Chemical and antioxidant diversity of essential oils of some Salvia species from Turkey, " Biochemical Systematics and Ecology, vol.106, pp.104575, 2023.
• [6] A. Kaya, M. Dinc, S. Dogu, and B. Demirci, "Compositions of essential oils of Salvia adenophylla, Salvia pilifera, and Salvia viscosa in Turkey, " Journal of Essential Oil Research, vol. 29, no. 3, pp.233-239, 2017.
• [7] M. Kosar, F. Goger, and K. H. C. Baser, "In vitro antioxidant properties and phenolic composition of Salvia halophila Hedge from Turkey, " Food Chemistry, vol.129, pp.374-379, 2011.
• [8] S. D. Hatipoglu, N. Zorlu, T. Dirmenci, A. C. Goren, T. Ozturk, and G. Topcu, "Determination of volatile organic compounds in fourty five Salvia species by thermal desorption-GC-MS Technique, " Records of Natural Products, vol.10, no.6, pp. 659-700, 2016.
• [9] G. Topcu, "Bioactive triterpenoids from Salvia species," Journal of Natural Products, vol. 69, pp. 482-487, 2006.
• [10] G. P. P. Kamatou, N. P. Makunga, W. P. N. Ramogola, A. M.Viljoen, "South African Salvia species: a review of biological activities and phytochemistry, " Journal of Ethnopharmacology, vol. 119, pp. 664–672, 2008.
• [11] M. S. Kocak, C. Sarikurkcu, M. Cengiz, S. Kocak, M. C. Uren, and B. Tepe, "Salvia cadmica: Phenolic composition and biological activity," Industrial Crops and Products, vol. 85, pp. 204-212, 2016.
• [12] M. Kursat, I. Emre, O. Yilmaz, and P. Erecevit. "Antioxidant and antimicrobial activity in the seeds of Origanum vulgare L. subsp. gracile (C. Koch) Ietswaart and Origanum acutidens (Hand.-Mazz.) Ietswaart from Turkey, " Grasas y Aceites, vol. 62, no. 4, 410-417, 2011.
• [13] Y. G. Zu, C. Y. Li, Y. J. Fu, and C. J. Zhao, "Simultaneous determination of catechin, rutin, quercetin kaempferol and isorhamnetin in the extract of sea buckthorn (Hippophae rhamnoides L.) leaves by RP-HPLC with DAD," Journal of Pharmaceutical and Biomedical Analysis,vol. 41, pp. 714-719, 2006.
• [14] C. M. Liyana-Pathiranan, and F. Shahidi, "Antioxidant activity of commercial soft and hard wheat (Triticum aestivum L.) as affected by gastric pH conditions," Journal of Agricultural and Food Chemistry, vol. 53, pp. 2433–2440, 2005.
• [15] R. Ree, N. Pellegrini, A. Proteggente, A. Pannala, M. Yang, and C. Rice-Evans, "Antioxidant activity applying an improved ABTS radical cation decolorization assay, " Free Radical Biology and Medicine, vol. 26, pp.1231–1237, 1999.
• [16] E. Skotti, E. Anastasaki, G. Kanello, M. Polissiou, P. A.Tarantilis, "Total phenolic content, antioxidant activity and toxicity of aqueous extracts from selected Greek medicinal and aromatic plants, " Industrial Crops and Products, vol. 53, pp. 46-54, 2014.
• [17] V. L. Singleton, R. Orthofer, and R. M. Lamuela-Raventos, "Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent, " Methods Enzymol, vol. 299, pp. 152–178, 1999.
• [18] H. H. Robya, M. A. Sarhana, K. A. Selima, K. I. Khalel, "Evaluation of antioxidant activity, total phenols and phenolic compounds in thyme (Thymus vulgaris L.), sage (Salvia officinalis L.), and marjoram (Origanum majorana L.) extracts, " Industrial Crops and Products, vol. 43, pp. 827–831, 2013.
• [19] T. C. P. Dinis, V. M. C. Madeira, and M. L M. Almeida, "Action of phenolic derivates (acetoaminophen, salycilate and 5-aminosalycilate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers, " Archives of Biochemistry and Biophysics, vol. 315, pp. 161-169, 1994.
• [20] K. Shimoi, S. Masuda, M. Furugoru, S. Esaki, N. Kinae, "Radioprotective effect of antioxidative flavonoids in X-ray irradiated mice, " Carcinogenesis, vol. 15, pp. 2669-2672, 1994.
• [21] S. Keser, E. Demir, and O. Yılmaz, "Phytochemicals and Antioxidant activity of the almond kernel (Prunus dulcis Mill.) from Turkey", Journal of the Chemical Society of Pakistan, vol. 36, pp.534-541, 2014.
• [22] C. M. Collins and P. M. Lyne, Microbiological Methods, Buttermorths & Co (Publishers) Ltd. Pp. 450. London, 1987.
• [23] S. Kivrak, T. Gokturk, I. Kivrak, E. Kaya, and E. Karababa, "Investigation of phenolic profiles and antioxidant activities of some Salvia species commonly grown in southwest Anatolia using UPLC-ESI-MS/MS," Food Science and Technology, pp. 1-9, 2018.
• [24] S. Francik, R. Francik, U. Sadowska, B. Bystrowska, A. Zawislak, A. Knapczyk, A. Nzeyimana, "Identification of phenolic compounds and determination of antioxidant activity in extracts and ınfusions of Salvia leaves," Materials, vol. 13, pp. 1-15, 2020.
• [25] G. Zengin, E. J. Llorent-Martinez, M. L. Fernandez-de Cordova, M. B. Bahadoric, A. Mocand, M. Locatellif, and A. Aktumsek, "Chemical composition and biological activities of extracts from three Salvia species: S. blepharochlaena, S. euphratica var. leiocalycina, and S. verticillata subsp. amasiaca, " Industrial Crops and Products, vol. 111, pp.11-21. 2018.
• [26] O. Yumrutas, A. Sokmen, H. A. Akpulat, N. Ozturk, D. Daferera, M. Sokmen, and B.Tepe, "Phenolic acid contents, essential oil compositions and antioxidant activities of two varieties of Salvia euphratica from Turkey, " Natural Product Research, vol. 26, no.19, pp. 1848-1851, 2012.
• [27] S. S. Erdogan, U. Karik, K. H. C. Baser, "The Determination of antioxidant activity of some sage populations of in the Marmara region," Turkish Journal of Agricultural and Natural Sciences, vol .2, pp. 1877-1882, 2014.
• [28] B. Tepe, M. Sokmen, H. A. Akpulat, and A. Sokmen, "Screening of the antioxidant potentials of six Salvia species from Turkey," Food Chemistry, vol. 95, pp.200–204, 2006.
• [29] I. E. Orhan, F. S. Senol, T. Ercetin, A. Kahraman, F. Celep, G. Akaydin, B. Sener, M. Dogan, "Assessment of anticholinesterase and antioxidant properties of selected sage (Salvia) species with their total phenol and flavonoid contents, " Industrial Crops and Products, vol. 41, pp. 21-30.
• [30] L. Svydenko, O. Vergun, E. Ivanišová, O. Korablova, and K. F. Šramková, "Polyphenol compounds and antioxidant activity of Salvia officinalis L. and Salvia sclarea L., " Agrobiodiversity for Improving Nutrition, Health and Life Quality, vol. 6, no. 2, pp.139–148, 2022.
• [31] A. Onder, M. N. Izgi, A. S. Cinar, G. Zengin, and M. A. Yilmaz, "The characterization of phenolic compounds via LC-ESI-MS/MS, antioxidant, properties enzyme inhibitory activities of Salvia absconditiflora, Salvia sclarea, and Salvia palaestina: A comparative analysis, " South African Journal of Botany, vol.150, pp. 313-322, 2022.
• [32] B. Culhaoglu, S. D. Hatipoglu, A. A. Donmez, G. Topcu, "Antioxidant and anticholinesterase activities of lupane triterpenoids and other constituents of Salvia trichoclada, " Medicinal Chemistry Research, vol. 24, pp. 3831–3837, 2015.
• [33] M. Ben Farhat, R. Chaouch-Hamada, J. A. Sotomayor. et al., "Antioxidant potential of Salvia officinalis L. residues as affected by the harvesting time", Industrial Crops and Products, vol. 54, pp.78-85, 2014.
• [34] S. Duletic-Lausevic, A. Alimpic, D. Pavlovic, P. D. Marin, and D. Lakusic, "Salvia officinalis of different origins Antioxidant activity, phenolic and flavonoid content of extracts. Agro Food Industry Hi Tech, vol. 27, no.1, pp. 52-55, 2016.
• [35] G. Yilmaz, N. Eruygur, G. Eksi Bona, M. Bona, M. Akdeniz, M. A. Yilmaz, A. Ertas, "Phytochemical analysis, antioxidant, and enzyme inhibition activity of five Salvia taxa from Turkey", South African Journal of Botany, vol. 152, pp. 212-221, 2023.
• [36] R. S. Marinel, E. A. Moraes, S. A. Lenquiste, A. T. Godoy, M. N. Eberlin, M. R. Marostica, "Chemical characterization and antioxidant potential of chilean chia seeds and oil (Salvia hispanica L. ,". LWT - Food Science and Technology, vol. 59, pp.1304-1310, 2014.
• [37] O. Firuzia, K. Javidniaa, M. Gholamia, R. Soltania Mir, "Antioxidant activity and total phenolic content of 24 Lamiaceae species growing in Iran," Natural Product Communications, vol. 5, no.2, pp. 261-264, 2010.
• [38] Z. A. Jeshvaghania, M. Rahimmalek, M. Talebi, S. Amir, H. Golli, "Comparison of total phenolic content and antioxidant activity in different Salvia species using three model systems, " Industrial Crops and Products, vol. 77, pp. 409-414, 2015.
• [39] I. E. Orhan, F.S. Senol, N. Ozturk, G. Akaydin, B. Sener, "Profiling of in vitro neurobiological effects and phenolic acids of selected endemic Salvia species, " Food Chemistry, vol.132: pp. 1360-1367, 2012.
• [40] F. S. Senol, I. Orhan, F. Celep, A. Kahraman, M. Dogan, G. Yılmaz, B. Sener, "Survey of 55 Turkish Salvia taxa for their acetylcholinesterase inhibitory and antioxidant activities," Food Chemistry, vol. 120, pp. 34–43, 2010.
• [41] V. Adımcılar, Z. Kalaycıoğlu, N. Aydoğdu, T. Dirmenci, A. Kahraman, F.B. Erim, “Rosmarinic and carnosic acid contents and correlated antioxidant and antidiabetic activities of 14 Salvia species from Anatolia”, Journal of Pharmaceutical and Biomedical Analysis, vol. 175, 112763, 2019.
• [42] A. Onder, M.N. Izgi, A.S. Cinar, G. Zengin, M.A.Yilmaz, “The characterization of phenolic compounds via LC-ESI-MS/MS, antioxidant, properties enzyme inhibitory activities of Salvia absconditiflora, Salvia sclarea, and Salvia palaestina: A comparative analysis” South African Journal of Botany, vol. 150, 313-322, 2022.
• [43] I. Gulcin, M. T. Uguz, M. Oktay, S. Beydemir, I. O. Kufrevioglu, "Evaluation of the antioxidant and antimicrobial activities of clary sage (Salvia sclarea L.), " Turkish Journal of Agriculture and Forestry, vol. 28, pp. 25-33, 2004.
• [44] S. Guzel, Y. Ozay, M. Kumas, C. Uzun, E. Gokal Ozkorkmaz, Z. Yıldırım, M. Ulger, G. Guler, A. Celik, Y. Camlıca, A. Kahraman, "Fito (Wound healing properties, antimicrobial and antioxidant activities of Salvia kronenburgii Rech. f. and Salvia euphratica Montbret, Aucher & Rech. f. var. euphratica on excision and incision wound models in diabetic rats", Biomedicine & Pharmacotherapy, vol. 111, pp. 1260-1276, 2019.
• [45] H. Norouzi-Arasi, I. Yavari, F. Chalabian, P. Baghaii, V. Kiarostami, M. Nasrabadi, A. Aminkhani, "Volatile constituents and antimicrobial activities of Salvia suffruticosa Montbr. & Auch. ex Benth. from Iran," Flavour and Fragrance Journal, vol. 20, no. 6, pp. 633-636, 2005.