Acute and Chonic Effects of Silver Nanoparticles (AgNPs) on Unio delicatus

Year 2024, , 101 - 106, 24.03.2024

İlker Şimşek , Pınar Arslan , Çağlan Günal , Özgür Kuzukıran , Ayhan Filazi

https://doi.org/10.17798/bitlisfen.1351240

Abstract

The widespread use of silver nanoparticles (AgNPs) including water filters, paints, cosmetics, deodorants, clothing, textiles, food packaging, electrical appliances and medical devices inevitably leads to their release into the natural environment, bioaccumulation in organisms and persistent accumulation in natural aquatic systems. The aim of this study is to investigate the acute and chronic effects of silver nanoparticles, which can contaminate aquatic ecosystems, in freshwater mussels, one of the aquatic invertebrate organisms. The model organism of the study, Unio delicatus, was obtained from Gölbaşı Lake (Hatay). After that acclimation was performed in the laboratory for two weeks. The mussels were then exposed to 1 and 10 mg/L AgNPs for 7 and 21 days. At the end of the exposure period, hemolymph and tissue samples of the mussels were taken. Total hemocyte count from hemolymph samples, lipid peroxidation and glutathione levels from tissue samples (digestive gland and gill) were investigated. Acute exposure resulted in an increase in the total hemocyte counts, while chronic exposure resulted in a significant decrease (P<0.05). Changes in lipid peroxidation and glutathione levels in gill and digestive gland tissues of mussels also occurred but not significantly. In conclusion, AgNPs caused changes in physiological and biochemical parameters of freshwater mussels.

Keywords

Exposure, Metallic Nanoparticles, Silver, Test Organism, Unionidae

References

• [1] A. Ale, G. Liberatori, M. L. Vannuccini, E. Bergami, S. Ancora, G. Mariotti, N. Bianchi, J. M.Galdopórpora, M. F. Desimone, J. Cazenave, and I. Corsi, ‘‘Exposure to a nanosilver-enabled consumer product results in similar accumulation and toxicity of silver nanoparticles in the marine mussel Mytilus galloprovincialis,’’ Aquatic Toxicology, vol. 211, pp. 46–56, March 2019.
• [2] J. Zhao, X. Wang, S. A. Hoang, N. S. Bolan, M. B. Kirkham, J. Liu, X. Xia, and Y. Li, ‘‘Silver nanoparticles in aquatic sediments: Occurrence, chemical transformations, toxicity, and analytical methods,’’ Journal of Hazardous Materials, vol. 418, p. 126368, September 2021.
• [3] M. Auguste, C. Ciacci, T. Balbi, A. Brunelli, V. Caratto, A. Marcomini, R. Cuppini, and L. Canesi, ‘‘Effects of nanosilver on Mytilus galloprovincialis hemocytes and early embryo development,’’ Aquatic Toxicology, vol. 203, pp. 107–116, August 2018.
• [4] A. G. Şahin, and M. Karatepe, 2022: ‘‘Vitamins A, E, C, β -carotene contents and MDA level of freshwater mussel, (Unio elongatulus eucirrus Bourguignat 1860) in the Karakaya Dam Lake,’’ Ege Journal of Fisheries and Aquatic Sciences, vol. 39, no. 2, pp. 120-124, June 2022.
• [5] A. G. Şahin, M. R. Gül, and M. Küçükyılmaz, ‘‘Karakaya Baraj Gölü'ndeki Tatlı Su Midyesi (Unio elongatulus eucirrus Bourguignat 1860)’nin Ağır Metal Düzeylerinin Belirlenmesi,’’ Fırat Üniversitesi Fen Bilimleri Dergisi, vol. 28, no. 1, pp. 13-19, January 2016.
• [6] M. Güler, D. Çoban, and B. Kırım, ‘‘Unio terminalis (Bivalvia: Unionidae)' in üreme biyolojisi üzerine gözlemler,’’ Ege Journal of Fisheries and Aquatic Sciences, vol. 34, no. 3, pp. 303-309, June 2017.
• [7] F. Gagne, J. Auclair, P. Turcotte, and C. Gagnon, ‘‘Sublethal Effects of Silver Nanoparticles and Dissolved Silver in Freshwater Mussels,’’ Journal of Toxicology and Environmental Health, Part A, vol. 76, no. 8, pp. 479–490, February 2013.
• [8] P. Arslan, ‘‘Determinations of the effects of cyfluthrin on the hemocytes parameters of freshwater mussel (Unio delicatus),’’ Ege Journal of Fisheries and Aquatic Sciences, vol. 39, no. 1, pp. 39-45, January 2022.
• [9] P. Arslan, and A. Ç. Günal, ‘‘Does Fipronil Affect on Aquatic Organisms? Physiological, Biochemical, and Histopathological Alterations of Non-Target Freshwater Mussel Species,’’ Water, vol. 15, no. 2, p. 334, January 2023.
• [10] T. Gomes, J. P. Pinheiro, I. Cancio, C. G. Pereira, C. Cardoso, and M. J. Bebianno, ‘‘Effects of copper nanoparticles exposure in the mussel Mytilus galloprovincialis,’’ Environmental science & technology, vol. 45, no. 21, pp. 9356-9362, October 2011.
• [11] T. Gomes, O. Araújo, R. Pereira, A. C. Almeida, A. Crav, and M. J. Bebianno, ‘‘Genotoxicity of copper oxide and silver nanoparticles in the mussel Mytilus galloprovincialis,’’ Marine environmental research, vol. 84, pp. 51-59, March 2013.
• [12] E. G. Canli, A. Baykose, L. H. Uslu, and M. Canli, ‘‘Changes in energy reserves and responses of some biomarkers in freshwater mussels exposed to metal-oxide nanoparticles,’’ Environmental Toxicology and Pharmacology, vol. 98, p.104077, March 2003.
• [13] A. Katsumiti, D. Gilliland, I. Arostegui, and M. P. Cajaraville, ‘‘Mechanisms of toxicity of Ag nanoparticles in comparison to bulk and ionic Ag on mussel hemocytes and gill cells,’’ PLoS One, vol. 10, no. 6, p. e0129039, June 2015.
• [14] M. Munari, J. Sturve, G. Frenzilli, M. B. Sanders, P. Christian, M. Nigro, and B. P. Lyons, ‘‘Genotoxic effects of Ag2S and CdS nanoparticles in blue mussel (Mytilus edulis) haemocytes,’’ Chemistry and Ecology, vol. 30, no. 8, pp. 719-725, March 2014.
• [15] REACH, ‘‘Guidance on Information Requirements and Chemical Safety Assessment,’’ Chapter R.7b: Endpoint specific guidance Retrieved from https://echa.europa.eu/documents/10162/17224/information_requirements_r7b_en.pdf/1a551efc-bd6a-4d1f-b719-16e0d3a01919?t=1498476047712, 2017.
• [16] H. Y. Yavuzcan, A. Ç. G. Benli, ‘‘Nitrite toxicity to crayfish, Astacus leptodactylus, the effects of sublethal nitrite exposure on hemolymph nitrite, total hemocyte counts, and hemolymph glucose,’’ Ecotoxicology and Environmental Safety, vol. 59, no. 3, pp. 370–375, November 2004.
• [17] M. Mihara, and M. Uchiyama, ‘‘Determination of malonaldehyde precursor in tissues by thiobarbituric acid test,’’ Analytical Biochemistry, vol. 86, no. 1, pp. 271-278, May 1978.
• [18] G. L. Ellman, ‘‘Tissue sulfhydryl groups,’’ Archives of Biochemistry and Biophysics, vol. 82, pp. 70-77, May 1959.
• [19] H. E. Parry, and R. K. Pipe, ‘‘Interactive effects of temperature and copper on immunocompetence and disease susceptibility in mussels (Mytilus edulis),’’ Aquatic toxicology, vol. 69, no. 4, pp. 311-325, September 2004.
• [20] Y. Sui, H. Kong, Y. Shang, X. Huang, F. Wu, M. Hu, and Y. Wang, ‘‘Effects of short-term hypoxia and seawater acidification on hemocyte responses of the mussel Mytilus coruscus,’’ Marine pollution bulletin, vol. 108, no. 1-2, pp. 46-52, July 2016.
• [21] X. Huang, D. Lin, K. Ning, Y. Sui, M. Hu, W. Lu, and Y. Wang, ‘‘Hemocyte responses of the thick shell mussel Mytilus coruscus exposed to nano-TiO2 and seawater acidification,’’ Aquatic Toxicology, vol. 180, pp. 1-10, September 2016.
• [22] T. Wang, X. Huang, X. Jiang, M. Hu, W. Huang, and Y. Wang, ‘‘Differential in vivo hemocyte responses to nano titanium dioxide in mussels: Effects of particle size,’’ Aquatic Toxicology, vol. 212, pp. 28-36, July 2019.
• [23] D. Tsikas, ‘‘Assessment of lipid peroxidation by measuring malondialdehyde (MDA) and relatives in biological samples: Analytical and biological challenges,’’ Analytical biochemistry, vol. 524, pp. 13-30, October 2017.
• [24] F. Gagne, J. Auclair, P. Turcotte, and C. Gagnon, ‘‘Sublethal Effects of Sılver Nanopartıcles and Dıssolved Silver in Freshwater Mussels,’’ Journal of Toxicology and Environmental Health, Part A, vol. 76, pp.479–490, February 2013.
• [25] E.A. Almeida, A. C. D. Bainy, A. L. Dafre, O. F. Gomes, M. H. Medeiros, and P. Di Mascio, ‘‘Oxidative stress in digestive gland and gill of the brown mussel (Perna perna) exposed to air and re-submersed,’’ Journal of Experimental Marine Biology and Ecology, vol. 318, no.1, pp. 21-30, May 2005.
• [26] S. Tedesco, H. Doyle, J. Blasco, G. Redmond, and D. Sheehan, ‘‘Oxidative stress and toxicity of gold nanoparticles in Mytilus edulis,’’ Aquatic toxicology, vol. 100, no. 2, pp. 178-186, March 2010.
• [27] M. Dellali, A. Khallouli, A. H. Harrath, F. Falodah, S. Alwasel, H. Beyrem, and F. Boufahja, ‘‘Effects of Au/TiO 2 metallic nanoparticles on Unio ravoisieri: Assessment through an oxidative stress and toxicity biomarkers,’’ Environmental Science and Pollution Research, vol. 28, pp. 18176-18185, January 2021.
• [28] S. E. Gürkan, ‘‘Impact of nickel oxide nanoparticles (NiO) on oxidative stress biomarkers and hemocyte counts of Mytilus galloprovincialis,’’ Biological Trace Element Research, vol. 200, no. 7, pp. 3429-3441, March 2022.
• [29] L. Canesi, A. Viarengo, C. Leonzio, M. Filippelli, and G. Gallo, ‘‘Heavy metals and glutathione metabolism in mussel tissues,’’ Aquatic Toxicology, vol. 46, no. 1, pp. 67-76, June 1999.
• [30] F. Regoli, and G. Principato, ‘‘Glutathione, glutathione-dependent and antioxidant enzymes in mussel, Mytilus galloprovincialis, exposed to metals under field and laboratory conditions: implications for the use of biochemical biomarkers,’’ Aquatic toxicology, vol. 31, no. 2, pp. 143-164, February 1995.
• [31] H. Falfushynska, L. Gnatyshyna, I. Yurchak, I. Sokolova, and O. Stoliar, ‘‘The effects of zinc nanooxide on cellular stress responses of the freshwater mussels Unio tumidus are modulated by elevated temperature and organic pollutants,’’ Aquatic Toxicology, vol. 162, pp. 82-93, May 2015.
• [32] X. Huang, Z. Liu, Z. Xie, S. Dupont, W. Huang, F. Wu, and Y. Wang, ‘‘Oxidative stress induced by titanium dioxide nanoparticles increases under seawater acidification in the thick shell mussel Mytilus coruscus,’’ Marine environmental research, vol. 137, pp. 49-59, June 2018.