Investigation the Cytotoxic and Antimicrobial Effect of Ranunculus Poluninii

Year 2024, , 393 - 399, 29.06.2024

İrem Nur Menevşe , Irmak Icen Taskın , Pelin Yılmaz Sancar , Murat Kürşat

https://doi.org/10.17798/bitlisfen.1403830

Abstract

Ranunculus plant species, one of the endemic plants, are used in the treatment of various diseases such as febrile diseases, rheumatism and inflammatory rashes due to their pharmacological and toxicological activities. Considering these features, it was aimed to investigate the cytotoxic effects of Ranunculus poluninii on cancer cell lines, bacteria and yeasts. For this purpose, the extract of the plant was obtained by methanol extraction. Different concentrations of stock were prepared from this plant and applied to A549 and HCT116 cancer cell lines, and MTT assay was performed to determine cell viability. In addition, MIC values were determined by applying the prepared plant extract to Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus, Candida albicans and Candida glabrata. Our results showed that Ranunculus poluninii was reduced cell viability to 83.7% at 100 µg/mL and 79% at 200 µg/mL on A549 and HCT116 respectively. In addition, it decreased both Candida albicans and Candida glabrata growth to 91.5% at 800 µg/m and 95.1% at 50 µg/mL respectively. Our results suggest that Ranunculus poluninii has anticancer effect and antifungal activity.

Keywords

: Antimicrobial, cytotoxic, MTT, MIC, Ranunculus poluninii

Supporting Institution

TUBITAK

Project Number

1919B012217255

References

• [1] A. R. Amala Dev and J. Sonia Mol, “Citrus essential oils: A rational view on its chemical profiles, mode of action of anticancer effects/antiproliferative activity on various human cancer cell lines,” Cell Biochem. Biophys., vol. 81, no. 2, pp. 189–203, 2023.
• [2] H. Sung et al., “Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries,” CA Cancer J. Clin., vol. 71, no. 3, pp. 209–249, 2021.
• [3] S. Z, “The natural products and healthy life,” J. Tradit. Med. Clin. Naturop., vol. 07, no. 02, 2018.
• [4] S. Almazov, P. Y. Sancar, M. Kürşat, O. Gedik, and Ş. Civelek, “Cytogenetic Investigation of Some Ranunculus L. Species Distributed in Bitlis and its Surroundings,” Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi, vol. 6, no. Ek Sayı, pp. 199–210, Dec. 2023, doi: 10.47495/okufbed.1149043.
• [5] D.-C. Hao, P.-G. Xiao, H.-Y. Ma, Y. Peng, and C.-N. He, “Mining chemodiversity from biodiversity: pharmacophylogeny of medicinal plants of Ranunculaceae,” Chin. J. Nat. Med., vol. 13, no. 7, pp. 507–520, 2015.
• [6] H. Özçelik, “Endemik Ranunculus poluninii ve Ranunculus crateris Ranunculaceae Populasyonları Üzerinde Taksonomik ve Ekolojik Araştırmalar,” Süleyman Demirel Üniv. Fen Dergisi, vol. 11, no. 1, pp. 1–41, 2016.
• [7] Y.-K. Goo, “Therapeutic potential of Ranunculus species (ranunculaceae): A literature review on traditional medicinal herbs,” Plants, vol. 11, no. 12, p. 1599, 2022.
• [8] D. Mares, “Antimicrobial activity of protoanemonin, a lactone from ranunculaceous plants,” Mycopathologia, vol. 98, no. 3, pp. 133–140, 1987.
• [9] T. Byambaa et al., “In vitro Study Results of the Ranunculus Repens L.’s Effect on Some Cancer Cells,” In vitro, vol. 29, no. 11, 2022.
• [10] K. You, Y. Liu, L. Chen, H. Ye, and W. Lin, “Radix ranunculus temate saponins sensitizes ovarian cancer to Taxol via upregulation of miR let 7b,” Exp. Ther. Med., vol. 23, no. 5, 2022.
• [11] S. O. Udegbunam, R. I. Udegbunam, C. C. Muogbo, M. U. Anyanwu, and C. O. Nwaehujor, “Wound healing and antibacterial properties of methanolic extract of Pupalia lappacea Juss in rats,” BMC Complement. Altern. Med., vol. 14, no. 1, 2014.
• [12] N. Sharma, G. Arya, R. Kumari, N. Gupta, and S. Nimesh, “Evaluation of anticancer activity of silver nanoparticles on the A549 human lung carcinoma cell lines through Alamar blue assay,” Bio Protoc., vol. 9, no. 1, 2019.
• [13] F. M. Ausubel, R. Brent, R. Kingston, D. Moore, J. Smith, and K. Struhl, “Current Protocols in Molecular Biology,” Current Protocols in Molecular Biology, 1991.
• [14] M. R. Green and J. Sambrook, Molecular cloning: a laboratory manual. V. 1. Cold Spring Harbor. N.Y.: Cold Spring Harbor Laboratory Press, 2012.
• [15] F. Bray, M. Laversanne, E. Weiderpass, and I. Soerjomataram, “The ever‐increasing importance of cancer as a leading cause of premature death worldwide,” Cancer, vol. 127, no. 16, pp. 3029–3030, 2021.
• [16] W. Li, “Botanical drugs: The next new new thing?,” 2002. Available at: http://nrs.harvard.edu/urn-3:HUL.InstRepos:8965577 (accessed June 20, 2023).
• [17] G. M. Cragg and D. J. Newman, “Plants as a source of anti-cancer agents,” J. Ethnopharmacol., vol. 100, no. 1–2, pp. 72–79, 2005.
• [18] M. C. Wani, H. L. Taylor, M. E. Wall, P. Coggon, and A. T. McPhail, “Plant antitumor agents. VI. Isolation and structure of taxol, a novel antileukemic and antitumor agent from Taxus brevifolia,” J. Am. Chem. Soc., vol. 93, no. 9, pp. 2325–2327, 1971.
• [19] A. B. Kunnumakkara, P. Anand, and B. B. Aggarwal, “Curcumin inhibits proliferation, invasion, angiogenesis and metastasis of different cancers through interaction with multiple cell signaling proteins,” Cancer Lett., vol. 269, no. 2, pp. 199–225, 2008.
• [20] M. Fang, T. Shinomiya, and Y. Nagahara, “Cell death induction by Ranunculus ternatus extract is independent of mitochondria and dependent on Caspase-7,” 3 Biotech, vol. 10, no. 3, 2020.
• [21] Narankhuu Ragchaasuren et al., “Antitumor Effects Of Phytochemicals Of Hot Three Plants On Gastric Adenocarcinoma,” bioRxiv (Cold Spring Harbor Laboratory), Nov. 2021, doi: https://doi.org/10.1101/2021.11.21.469373.
• [22] J. L. Rodriguez-Tudela et al., “EUCAST Definitive Document EDef 7.1: method for the determination of broth dilution MICs of antifungal agents for fermentative yeasts: Subcommittee on Antifungal Susceptibility Testing (AFST) of the ESCMID European Committee for Antimicrobial Susceptibility Testing (EUCAST),” Clinical Microbiology and Infection, vol. 14, no. 4, pp. 398–405, 2008.
• [23] F. R. Cockerill and Clinical and Laboratory Standards Institute, Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically: approved standard. Wayne, Pa: Clinical and Laboratory Standards Institute, 2012.
• [24] E. K. Barbour, M. Al Sharif, V. K. Sagherian, A. N. Habre, R. S. Talhouk, and S. N. Talhouk, “Screening of selected indigenous plants of Lebanon for antimicrobial activity,” Journal of Ethnopharmacology, vol. 93, no. 1, pp. 1–7, Jul. 2004, doi: https://doi.org/10.1016/j.jep.2004.02.027.
• [25] S. Nazir, “In vitro Screening of Ranunculus muricatus for Potential Cytotoxic and Antimicrobial Activities,” Global Journal of Pharmacology, 2014.