Ecophysiological Profiling of Ignatzschineria larvae Isolated from an Aquatic Insect: pH, Salinity, Thermal, Radiation, and Antibiotic Tolerance

Mehmet Bektaş; Figen Orhan; Özlem Bariş

doi:10.17798/bitlisfen.1783872

Ecophysiological Profiling of Ignatzschineria larvae Isolated from an Aquatic Insect: pH, Salinity, Thermal, Radiation, and Antibiotic Tolerance

Abstract

Ignatzschineria larvae are a recently characterized bacterium with limited ecophysiological data and are proposed as a potential microbial biomarker for insect colonization. We isolated I. larvae (strain MW602513) from the gut of an aquatic insect and assessed their tolerance and antibiotic susceptibility (Kirby-Bauer, CLSI/EUCAST) to pH (3-11), NaCl salinity (0.5-10 %), temperature (4-55°C) and ultraviolet radiation. Inhibition zones were interpreted according to current breakpoints. The isolate exhibited robust growth at pH 6-7, low salinity (≤ 2%) and moderate temperatures (30-37°C); there was no growth at 10°C or 50-55°C, and a significant growth reduction was observed at ≥ 5% NaCl. Bacteria were observed to not grow under UV radiation applied for 5 minutes. Resistance to various antibiotic groups tested was observed. The results demonstrate that the bacteria can adapt to mesophilic environments with low salinity and neutral to alkaline pH, highlighting their ecological resilience and potential impact on ecosystems.

Keywords

Ethical Statement

The study is complied with research and publication ethics.

Thanks

We would like to express our gratitude to the Eastern Anatolia High Technology Application and Research Centre (DAYTAM) at Atatürk University for allowing us to utilise their microscope and for their assistance with certain tests.

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Details

Primary Language

English

Subjects

Radiobiology, Hydrobiology, Bacteriology

Journal Section

Research Article

Authors

Mehmet Bektaş ^*
0000-0002-7420-6883
Türkiye

Figen Orhan
0000-0002-1839-3434
Türkiye

Özlem Bariş
0000-0002-2679-5599
Türkiye

Publication Date

March 24, 2026

Submission Date

September 14, 2025

Acceptance Date

December 17, 2025

Published in Issue

Year 2026 Volume: 15 Number: 1

DOI

https://doi.org/10.17798/bitlisfen.1783872

IZ

https://izlik.org/JA23SF99FA

Cite

RIS / Bibtex

APA

Bektaş, M., Orhan, F., & Bariş, Ö. (2026). Ecophysiological Profiling of Ignatzschineria larvae Isolated from an Aquatic Insect: pH, Salinity, Thermal, Radiation, and Antibiotic Tolerance. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi, 15(1), 159-168. https://doi.org/10.17798/bitlisfen.1783872

AMA

1.Bektaş M, Orhan F, Bariş Ö. Ecophysiological Profiling of Ignatzschineria larvae Isolated from an Aquatic Insect: pH, Salinity, Thermal, Radiation, and Antibiotic Tolerance. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi. 2026;15(1):159-168. doi:10.17798/bitlisfen.1783872

Chicago

Bektaş, Mehmet, Figen Orhan, and Özlem Bariş. 2026. “Ecophysiological Profiling of Ignatzschineria Larvae Isolated from an Aquatic Insect: PH, Salinity, Thermal, Radiation, and Antibiotic Tolerance”. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi 15 (1): 159-68. https://doi.org/10.17798/bitlisfen.1783872.

EndNote

Bektaş M, Orhan F, Bariş Ö (March 1, 2026) Ecophysiological Profiling of Ignatzschineria larvae Isolated from an Aquatic Insect: pH, Salinity, Thermal, Radiation, and Antibiotic Tolerance. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi 15 1 159–168.

IEEE

[1]M. Bektaş, F. Orhan, and Ö. Bariş, “Ecophysiological Profiling of Ignatzschineria larvae Isolated from an Aquatic Insect: pH, Salinity, Thermal, Radiation, and Antibiotic Tolerance”, Bitlis Eren Üniversitesi Fen Bilimleri Dergisi, vol. 15, no. 1, pp. 159–168, Mar. 2026, doi: 10.17798/bitlisfen.1783872.

ISNAD

Bektaş, Mehmet - Orhan, Figen - Bariş, Özlem. “Ecophysiological Profiling of Ignatzschineria Larvae Isolated from an Aquatic Insect: PH, Salinity, Thermal, Radiation, and Antibiotic Tolerance”. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi 15/1 (March 1, 2026): 159-168. https://doi.org/10.17798/bitlisfen.1783872.

JAMA

1.Bektaş M, Orhan F, Bariş Ö. Ecophysiological Profiling of Ignatzschineria larvae Isolated from an Aquatic Insect: pH, Salinity, Thermal, Radiation, and Antibiotic Tolerance. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi. 2026;15:159–168.

MLA

Bektaş, Mehmet, et al. “Ecophysiological Profiling of Ignatzschineria Larvae Isolated from an Aquatic Insect: PH, Salinity, Thermal, Radiation, and Antibiotic Tolerance”. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi, vol. 15, no. 1, Mar. 2026, pp. 159-68, doi:10.17798/bitlisfen.1783872.

Vancouver

1.Mehmet Bektaş, Figen Orhan, Özlem Bariş. Ecophysiological Profiling of Ignatzschineria larvae Isolated from an Aquatic Insect: pH, Salinity, Thermal, Radiation, and Antibiotic Tolerance. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi. 2026 Mar. 1;15(1):159-68. doi:10.17798/bitlisfen.1783872