Plumbago europaea L.’nın Besi Yeri Ortamındaki Saccharomyces cerevisiae Gelişimi Üzerine Etkileri

Year 2015, , 0 - 0, 22.12.2015

Burak Bircan , Sevda Kırbağ

https://doi.org/10.17798/beufen.32692

Abstract

Bu çalışmada; Plumbago europea’nın maya kültüründeki antioksidan etkileri araştırıldı. Malondialdehit (MDA) seviyesi, glutatyon, total protein ve süperoksit dismütaz (SOD) düzeylerinin değişebildiği gözlendi. Kültür ortamında bitkinin MDA ve total protein seviyesini Fenton reaktifi (FR) grubuna kıyasla yükselttiği ve glutatyon miktarını koruduğu belirlendi. Sodyum dodesil sülfatlı-Poliakrilamit jel elektroforezi (SDS-Page) analizinde ise SOD ve katalaz (CAT) miktarının FR grubuna kıyasla azaldığı tespit edildi.

References

• Faydaoğlu, E. and Sürücüoğlu, M.S., 2011. Geçmisten Günümüze Tıbbi ve Aromatik Bitkilerin Kullanılması ve Ekonomik Önemi, Kastamonu Üniversitesi Orman Fakültesi Dergisi, 11(1), 52-67.
• Demma, J., Hallberg, K., and Hellman, B., 2009. Genotoxicity of plumbagin and its effects on catechol and NQNO-induced DNA damage in mouse lymphoma cells, Toxicol In Vitro, 23(2), 266-71.
• Navarrete, C., Siles, A., Martinez, J.L., Calero, F., and Ramos, J., 2009. Oxidative stress sensitivity in Debaryomyces hansenii, FEMS Yeast Res, 9(4), 582-90.
• Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J., 1951. Protein measurement with the Folin phenol reagent, J Biol Chem, 193(1), 265-75.
• Pegg, R.B., 2001. Spectrophotometric measurement of secondary lipid oxidation products, Current protocols in food analytical chemistry, 1-18.
• Ellman, G.L., 1959. Tissue sulfhydryl groups, Arch Biochem Biophys, 82(1), 70-7.
• Wang, K.K., Posner, A., and Hajimohammadreza, I., 1996. Total protein extraction from cultured cells for use in electrophoresis and western blotting, BioTechniques, 20(4), 662-668.
• Laemmli, U.K., 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 227(5259), 680-5.
• Teshome, K., Gebre-Mariam, T., Asres, K., Perry, F., and Engidawork, E., 2008. Toxicity studies on dermal application of plant extract of Plumbago zeylanica used in Ethiopian traditional medicine, Journal of Ethnopharmacology, 117(2), 236-248.
• Folmer, V., Pedroso, N., Matias, A.C., Lopes, S.C.D.N., Antunes, F., Cyrne, L., and Marinho, H.S., 2008. H2O2 induces rapid biophysical and permeability changes in the plasma membrane of Saccharomyces cerevisiae, Biochimica et Biophysica Acta (BBA) - Biomembranes, 1778(4), 1141-1147.
• Matias, A.C., Pedroso, N., Teodoro, N., Marinho, H.S., Antunes, F., Nogueira, J.M., Herrero, E., and Cyrne, L., 2007. Down-regulation of fatty acid synthase increases the resistance of Saccharomyces cerevisiae cells to H2O2, Free Radic Biol Med, 43(10), 1458-65.
• Izawa, S., Inoue, Y., and Kimura, A., 1995. Oxidative stress response in yeast: effect of glutathione on adaptation to hydrogen peroxide stress in Saccharomyces cerevisiae, FEBS Letters, 368(1), 73-76.
• Penninckx, M., 2000. A short review on the role of glutathione in the response of yeasts to nutritional, environmental, and oxidative stresses, Enzyme and Microbial Technology, 26(9–10), 737-742.
• Pinheiro, R., Belo, I., and Mota, M., 2002. Oxidative stress response of Kluyveromyces marxianus to hydrogen peroxide, paraquat and pressure, Applied Microbiology and Biotechnology, 58(6), 842-847.