Mısır (Zea mays L.) Fidelerinde Kadmiyum Toksisitesi ile Nitrik Oksit Arasındaki Biyokimyasal İlişkiler

Year 2019, , 376 - 386, 28.06.2019

Songül Çanakcı Gülengül , Didem Deveci Fadime Karabulut

https://doi.org/10.17798/bitlisfen.459425

Abstract

Özet

Bu
çalışmada, 15 günlük mısır (Zea mays
L.) fidelerine, önceden farklı SNP (25 ve 50 µM) konsantrasyonları
uygulandıktan sonra, bu fidelerin farklı kadmiyum (25, 50 ve 75 µM)
konsantrasyonlarına karşı verdiği biyokimyasal cevaplar araştırıldı. Fidelere
yapılan tüm uygulamalar hidrofonik ortamda uygulandı. Cd uygulanan mısır
fidelerinin köklerinde ve yapraklarında SNP ön uygulamasız fidelerde, kontrole
kıyasla okside glutatyon (GSSG) ve redükte glutatyon (GSH) miktarlarında artma
ve SNP ön uygulamalı fidelerde ise azalma tespit edildi. Fidelere uygulanan
kadmiyum konsantrasyonları arttıkça, hem SNP ön uygulamasız, hem de SNP ön
uygulamalı fidelerde kontrol grubu fidelerine kıyasla 16:0 yapraklarda ve
köklerde artmıştır. Tek başına SNP uygulamalarında, 16:0 üzerinde kontrole
kıyasla, 50 µM SNP daha az bulunmuştur. Cd uygulamaları ile SNP ön uygulamasız
ve SNP ön uygulamalı fidelerde 16:1 yapraklarda genel olarak artarken, bazı konsantrasyonlarda
SNP ön uygulamasıyla bu artış hafifletilmiştir. Cd uygulamaları ile SNP ön
uygulamasız ve SNP ön uygulamalı fidelerde 18:0 köklerde ve 18:2 kök ve
yapraklarda artmıştır. Cd uygulamaları ile SNP ön uygulamasız fidelerde 18:3
yaprakta azalırken; SNP ön uygulamalı bazı fidelerde ise 18:3 miktarı
yapraklarda artmıştır. Genel olarak 50 µM SNP ön uygulamasının Cd toksisitesini
baskılama da 25 µM SNP’ den daha başarılı bulundu.

Keywords

Mısır (Zea mays L.), Nitrik oksit, Kadmiyum toksisitesi

References

• Kaynaklar
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