Arabidopsis thaliana Bitkisi ile Pseudomonas putida Bakterisi Arasındaki Etkileşimin in vitro Koşullarda Belirlenmesi

Year 2019, , 459 - 465, 28.06.2019

Özlem Akkaya

https://doi.org/10.17798/bitlisfen.496057

Abstract

A.
thaliana genomu dizilenmiş genetik, biyokimyasal ve
biyoteknolojik çalışmalarda yaygın olarak kullanılan model bir bitkidir. P. putida bakterisi ise bitki köklerinde
kolonize olabilen, genomu dizilenmiş, bitki büyümesini teşvik eden, patojenik
olmayan ve bitki-bakteri etkileşimlerinin anlaşılması için model olmaya aday
rizosferik bir bakteridir. Bu nedenle, bu çalışmanın amacı A. thaliana’nın in vitro
çimlenmesi ve fideciklerinin büyümesi üzerine P. putida’nın etkisinin değerlendirilmesidir. Bu amaçla, yüzey
sterilasyonu yapılmış A. thaliana
tohumlarının inokülasyonu için 2x10³-2x10⁵ CFU/ml
aralığındaki bakteri konsantrasyonları pipetleme metodu kullanılarak denenmiştir.
Ayrıca, aynı bakteriyel süspansiyonlar 3, 5, 10 ve 14 günlük in vitro koşullarda çimlendirilmiş
fideciklere de uygulanmıştır. Bu araştırma alanı, bitki-bakteri
etkileşimlerinde inokülasyonun öneminin anlaşılmasına önemli bir yarar ve bunun
yanı sıra daha sonraki kapsamlı çalışmalar için bir katkı sağlayacaktır.
Dahası, sonrasında bitki-bakteri etkileşimleri aracılığıyla biyoremediasyonun
başarılmasına yardımcı olacaktır.

Keywords

Arabidopsis, Pseudomonas putida

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