Karbontetraklorür İle Karaciğer Hasarı Oluşturulan Ratlarda Tribulus terrestris’in Antihiperlipidemik Etkisinin Araştırılması

Year 2019, , 779 - 786, 30.09.2019

Zafer Çambay , Naci Ömer Alayunt

https://doi.org/10.17798/bitlisfen.529739

Abstract

Bu araştırmada, karbon tetraklorür ile
deneysel olarak oluşturulan hepatotoksisite ile ratların kan lipid
parametrelerine karşı güçlü antioksidan etkisine sahip olan Tribulus Terrestris’in koruyucu
etkinliğinin ve antihiperlipidemik rolünün araştırılması amaçlanmıştır.
Çalışmada 35 adet 8-12 haftalık Wistar-Albino erkek rat kullanılmıştır. Çalışma
beş grupa ayrılıp her grupta 7 adet ratla başlanmıştır. Kontrol grubundaki
ratlar 14 gün standart yemle beslenmiştir. Diğer gruplardaki ratlara standart
yem ve 14 gün boyunca 0,8 ml/kg CCI4 intraperitoneal olarak uygulanmıştır. 3, 4
ve 5 gruplarda ki ratlara ise intraperitoneal olarak uygulanan 0,8 ml/kg CCI4’e
sonra 8 hafta boyunca Tribulus terrestris
bitki ekstraktı verilmiştir. Deney sonunda tüm sıçanlar uygun ortam ve
şartlarda dekapite edilerek ve çalışmanın sonuçlarının inceleneceği
parametreler için uygun kan örnekleri alınmıştır. Alınan kan örnekleri jelli
düz biyokimya tüplerine alınacak, 4000 rpm’de 5-10 dakika santrifüj edilerek
serum elde edilmiştir. Elde edilen serumlar otoanalizör yardımıyla lipid
parametreleri belirlenmiştir.  Karbontetraklorür
ile karaciğer hasarı oluşturan grup ile kontrol grubu lipid parametreleri total
kolesterol, trigliserit, düşük dansiteli lipoprotein kolesterol (LDL-K), çok
düşük dansiteli lipoprotein kolesterol (VLDL-K) ve yüksek dansiteli lipoprotein
kolesterol (HDL-K) değerlerinin arasında istatiksel bir fark olduğu saptandı
(P<0.05). 2 g/kg Tribulus
Terrestris’in verilen CCl₄ uygulanmış 5.gruptaki lipid
parametreleri ile kontrol grubu arasında anlamlı bir
fark saptanmadı (P>0.05). CCI4’ün neden olduğu oksidatif stres ve lipit
peroksidasyonu sonucu oluşturulan hiperlipidemiye karşı Tribulus Terrestris’in bitkisinin önemli düzeyde azaltıcı etkisinin olduğu
belirlenmiştir.

Keywords

CCl4, Tribulus Terrestris, antihiperlipidemi

References

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