The Preparation and Characterization of Electrospun PCL/PHBV Membranes as Wound Dressing Material

Abstract

In this study, fibrous membranes of Polycaprolactone (PCL), Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and PCL/PHBV blends in different weight ratios (100:0, 50:50, 75:25, 0:100) were prepared using different solvents (chloroform (CHCl₃), 1,1,1,3,3,3-Hexafluoro-2-propanol (HFIP) and their blends) via electrospinning technique as wound dressing materials. Structural, morphological, and thermal characterizations of membranes were performed by Fourier Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscopy (SEM) and Differential Scanning Calorimetry (DSC), respectively. Moreover, the water-uptake capacity of the membranes were analyzed using absorption test. Following that, human fibroblast cells were seeded on the selected membranes and in vitro cell viability and toxicity were monitored by MTT assay for 24, 48 and 72^th hours.  The seeded cell proliferation on membranes was conducted for 36, 72 and 120^th hours and the results were examined by SEM. The results of this study relieved that electrospun PCL/PHBV (75:25) blend membrane solved in HFIP can be used as wound dressing materials.    

Keywords

• PCL,PHBV,Electrospinning,Wound Dressing Material

Yara Örtü Malzemesi Olarak Elektroeğrilmiş PCL/PHBV Membranların Hazırlanması ve Karakterizasyonu

Abstract

Bu çalışmada, yara örtü malzemesi olarak polikaprolakton (PCL), poli(3-hidroksibütirik asit-ko-3-hidroksivalerik asit) (PHBV) ve ağırlıkça farklı oranlarda (100:0, 50:50, 75:25, 0:100) PCL/PHBV karışımları, farklı çözücüler (kloroform (CHCl3), 1,1,1,3,3,3-Hexfluoro-2-propanol (HFIP) ve bunların karışımları) kullanılarak elektroeğirme tekniği ile fibroz yapıda membranlar hazırlanmıştır. Tüm elektroeğrilmiş membranlar Fourier Dönüşümlü Kızılötesi Spektroskopisi (FT-IR), Diferansiyel Taramalı Kalorimetre (DSC) ve Taramalı Elektron Mikroskobu (SEM) ile yapı, morfoloji ve ısıl özellikleri açısından karakterize edilmiştir. Ayrıca, absorbsiyon testi ile sıvı tutma kapasiteleri analiz edilmiştir. Karakterizasyon basamağından sonra, seçilen membranların üzerine insan fibroblast hücreleri ekilmiş, in vitro hücre canlılık ve toksisite, MTT testi ile 24, 48 ve 72. saat için analiz edilmiştir. Membranların üzerine ekilen hücrelerin çoğalması 36, 72 ve 120. saat olmak üzere 3 farklı süre için incelenmiş, sonuçlar SEM ile gösterilmiştir. Elde edilen sonuçlar PCL/PHBV (75:25) karışım ile HFIP çözeltisinde elektroeğrilen membranın yara örtü malzemesi olarak kullanılabileceğini göstermiştir.

Keywords

• PCL,PHBV,Elektroeğirme,Yara Örtü Malzemesi

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