Determination of Antifungal Effect of Wood Vinegar Obtained from Hazelnut Shells Against Mold Factors in In-Vitro Conditions

Year 2018, Volume: 7 Issue: 2, 296 - 300, 28.12.2018

İbrahim Koç , Erdal Necip Yardım , Ali Çelik , Mehmet Mendeş , Hamit Mirtagioğlu Ayten Namlı

https://doi.org/10.17798/bitlisfen.425809

Cited By: 6

Abstract

This study was carried out at in
vitro conditions to determine the antifungal effect of the wood vinegar
obtained from hazelnut shells, on Aspergillus
niger and Penicillium digitatum
microfungi. Potato dextrose agar (PDA) containing concentrations of
1%, 3%, 5%, 7% and 10% of the wood vinegar was allowed to incubation for 7 days
at 24 ± 1 °C after inoculation of 5 mm diameter mycelium discs of microfungi. At the end of incubation, the colony diameters of the microfungi were
measured and the % inhibition rates of the wood vinegar, were calculated
according to the controls. Results of One-way ANOVA showed that there were
statistically significant differences among the doses (P=0.000). Based on the
Dunnett’s test results, it was determined that the differences between the
control group and the other doses of wood vinegar were statistically
significant except for the 1% mL wood vinegar dose. As a result; it is
possible to concluded that wood vinegar may be used for the prevention of mold
fungi. 

Keywords

Antifungal, Microfungi, Wood vinegar, In-Vitro

Fındık Kabuklarından Elde Edilmiş Odun Sirkesi’ nin In-Vitro Şartlarında Küf Etmenlerine Karşı Antifungal Etkisinin Belirlenmesi

Abstract

Bu çalışma fındık kabuklarından
elde edilen odun sirkesi’nin, in vitro şartlarında Aspergillus niger ve Penicillium digitatum mikrofunguslarına karşı
antifungal etkisini saptamak amacıyla yapılmıştır. Odun sirkesi’nin %1,
%3, %5, %7 ve %10 mL’lik konsantrasyonlarının dâhil edildiği Patates Dekstroz Agar (PDA)’a, mikrofungusların
5 mm çapındaki miselyum diskleri inoküle edildikten sonra
24±1 ^oC’de 7 gün boyunca inkübasyona bırakılmıştır. İnkübasyonun sonunda mikrofungusların
koloni çapları ölçülmüş ve kontrollere göre odun sirkesinin % engelleme
oranları hesaplanmıştır. Yapılan varyans analizi sonucunda dikkate alınan
uygulamalar arasında istatistiksel olarak önemli farkların bulunduğu
görülmüştür (P=0.000). Varyans analizi sonucunda uygulanan Dunnett testi
sonucunda ise kontrol grubu ile %1 mL odun sirkesi hariç, odun sirkesinin diğer
dozları arasında istatistiksel olarak önemli farkların bulunduğu görülmüştür. Sonuç
olarak; odun sirkesinin küf mantarlarını engellemede kullanılabileceği sonucuna
varılmıştır.

Keywords

Antifungal, Mikrofungus, Odun sirkesi, İn-Vitro

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