Karbontetraklorür İle Karaciğer Hasarı Oluşturulan Ratlarda Tribulus terrestris’in Antihiperlipidemik Etkisinin Araştırılması

Year 2019, Volume: 8 Issue: 3, 779 - 786, 30.09.2019

Zafer Çambay , Naci Ömer Alayunt

https://doi.org/10.17798/bitlisfen.529739

Abstract

Bu araştırmada, karbon tetraklorür ile
deneysel olarak oluşturulan hepatotoksisite ile ratların kan lipid
parametrelerine karşı güçlü antioksidan etkisine sahip olan Tribulus Terrestris’in koruyucu
etkinliğinin ve antihiperlipidemik rolünün araştırılması amaçlanmıştır.
Çalışmada 35 adet 8-12 haftalık Wistar-Albino erkek rat kullanılmıştır. Çalışma
beş grupa ayrılıp her grupta 7 adet ratla başlanmıştır. Kontrol grubundaki
ratlar 14 gün standart yemle beslenmiştir. Diğer gruplardaki ratlara standart
yem ve 14 gün boyunca 0,8 ml/kg CCI4 intraperitoneal olarak uygulanmıştır. 3, 4
ve 5 gruplarda ki ratlara ise intraperitoneal olarak uygulanan 0,8 ml/kg CCI4’e
sonra 8 hafta boyunca Tribulus terrestris
bitki ekstraktı verilmiştir. Deney sonunda tüm sıçanlar uygun ortam ve
şartlarda dekapite edilerek ve çalışmanın sonuçlarının inceleneceği
parametreler için uygun kan örnekleri alınmıştır. Alınan kan örnekleri jelli
düz biyokimya tüplerine alınacak, 4000 rpm’de 5-10 dakika santrifüj edilerek
serum elde edilmiştir. Elde edilen serumlar otoanalizör yardımıyla lipid
parametreleri belirlenmiştir.  Karbontetraklorür
ile karaciğer hasarı oluşturan grup ile kontrol grubu lipid parametreleri total
kolesterol, trigliserit, düşük dansiteli lipoprotein kolesterol (LDL-K), çok
düşük dansiteli lipoprotein kolesterol (VLDL-K) ve yüksek dansiteli lipoprotein
kolesterol (HDL-K) değerlerinin arasında istatiksel bir fark olduğu saptandı
(P<0.05). 2 g/kg Tribulus
Terrestris’in verilen CCl₄ uygulanmış 5.gruptaki lipid
parametreleri ile kontrol grubu arasında anlamlı bir
fark saptanmadı (P>0.05). CCI4’ün neden olduğu oksidatif stres ve lipit
peroksidasyonu sonucu oluşturulan hiperlipidemiye karşı Tribulus Terrestris’in bitkisinin önemli düzeyde azaltıcı etkisinin olduğu
belirlenmiştir.

Keywords

CCl4, Tribulus Terrestris, antihiperlipidemi

References

• Kaynaklar1. Çınar A, Yörük M, Meral İ, Kiliçalp D, Koç A, Ertekin A. 1999. The effects of carbon tetrachloride (CCl4) induced experimental acute and chronic intoxication on hystological structure of liver and some hematological values and electrocardiogram in Rabbits. Turk J Vet Anim Sci;23, 235-242.
• 2. Karakuş A, Değer Y, Yıldırım S. 2016. Protective effect of Silybum marianum and Taraxacum officinale extracts against oxidative kidney injuries induced by carbon tetrachloride in rats. Ren Fail. 39, 1-6.
• 3. Mukhopadhyay P, Mukherjee S, Ahsan K, Bagchi A, Pacher P, Das DK. 2010. Restoration of altered microRNA expression in the ischemic heart with Resveratrol. PLoS One, 23;5(12):e15705. doi: 10.1371/journal.pone.0015705.
• 4. Wang C, Fan RQ, Zhang YX, Nie H, Li K. 2016. Naringenin protects against isoniazid-and rifampicin-induced apoptosis in hepatic injury. World J Gastroenterol; 22, 9775-9783. 5. Bhuvaneswari R, Chidambaranathan N, Jegatheesan K. 2014. Hepatoprotective effect of embilica officinalis and its silver nanoparticles against CCl4 induced hepatotoxicity in wistar albino rats. DJNB, 9, 223-235.
• 6. Sotelo-Feliz JI, Martinez-Fong D, Muriel P, Santillan RL, Castillo D, Yahuaca P. 2002. Evaluation of the effectiveness of Rosmarinus officinalis (Lamiaceae) in the alleviation of carbon tetrachloride-induced acte hepatotoxicity in the rat. J Ethnopharmocol. 81, 145-154.
• 7. MacDonald-Wickks LK, Garg ML. 2003. Vitamin E supplemantation in the mitigation of carbon tetrachloride induced oxidative stres in rats. Nutr Biochem. 14, 211-218. 8. Galicia-Moreno M, Rodriguez-Rivera A, ReyesGordillo K, Segovia J, Shibayama M, Tsutsumi V, Muriel P. 2009. N-acetylcysteine prevents carbon tetrachloride-induced liver cirrhosis: role of liver transforming growth factor-beta and oxidative stress. Eur J Gastroenterol Hepatol. 21, 908-914.
• 9. Dündar Y. 2001. Fitokimyasallar ve sağlıklı yaşam. Kocatepe Tıp Derg; 2: 131-8. 10. Joshi, D.D, Uniyal, R.C. 2008. Different chemo types of Gokhru (Tribulus terrestris):A herb used for improving physique and physical performance. International Journal Of Gren Pharmacy. Volume: 2, Issue: 3, Pages: 158-161.
• 11. Li, J.X., Shi, Q., Xiong, Q.B., Prasain, J.K., Tezuka, Y., Hareyama, T., Wang, Z.T., Tanaka, K., Namba, T., Kadota, S. 1998. Tribulusamide A and B, new hepatoprotective lignanamides from the fruits of Tribulus terrestris: indications of cytoprotective activity in murine hepatocyte culture. Planta Med. 64(7): 628-31.
• 12. Liang D, Zhou Q, Gong W, Wang Y, Nie Z, He H, Li J, Wu J, Wu C, Zhang J. 2011. Studies on the antioxidant and hepatoprotective activities of polysaccharides from Talinum triangulare. Ethnopharmacol. J22;136(2):316-21.doi: 10.1016/j.jep.2011.04.047.
• 13. Manibusan MK, Odin M, Eastmond DA. 2007. Postulated carbon tetrachloride mode of action: A review. J Environ Sci Heal C; 25: 185-209.
• 14. Gnanaprakash K, Madhusudhana CC, Ramkanth S et al. 2010. Aqueous extract of Flacourtia indica prevents carbon tetrachloride induced hepatotoxicity in rat. Int J Biol Sci.6: 51-5.
• 15. Ökten A. 1998. Türkiye’de karaciger sirozunun etyolojisi. Hepotolojide Güncel Gelişmeler Sempozyum Kitabı: 67.
• 16. Wang H, Wei W, Wang NP. 2005. Melatonin ameliorates carbontetrachloride-induced hepatic fibrogenesis in rats via inhibition of oxidative stres. Life Sci. 77: 1902-1915.
• 17. Gochee PA, Johnsson JR, Clouston AD. 2003. Steatozis in Chronic Hepatitis C: association with increased Messenger RNA Expression of Collagen I, tumor necrosis factor-alpha and cytochrome P450 2E1. J Gastro Hepatolog. 18: 386-392.
• 18. Chin PL, Momand J, Pfeifer GP. 1997. In vivo evidence for binding of p53 to consensus binding sites in the p21 and GADD45 genes in response to ionizing radiation. Oncogene. 15: 87-100.
• 19. Li, M, Qu, W, Wang, Y, Wan, H, Tian, C. 2002. Hypoglycemic effect of saponin from Tribulus terrestris. Zhong Yao Cai. 25(6): 420-2.
• 20. Chu, S, Qu W, Pang, X, Sun B, Huang, X. 2003. Effect of saponin from Tribulus terrestris on hyperlipidemia. Zhong-Yao-Cai. 26(5): 341-4.
• 21. Phillips, O.A, Mathew, K.T, Oriowo, M.A. 2006. Antihypertensive and vasodilator effects of methanolic and aqueous extracts of Tribulus terrestris in rats. Journal of Ethnopharmacology 104 s: 351–355.
• 22. Tantawy, W.H, Hassain, L.A. 2007. Hypoglycemic and hypolipidemic effects of alcoholic extract of Tribulus alatus in streptozotocin-induced diabetic rats: A comparative study with T. terrestris (Caltrop). Indian J Exp Biol. 45:785- 90.
• 23. Grigorova S, Kashamov, B, Sredkova, V, Surdjiiska, S, Kurtenkov, A. 2008. Effect Of Tribulus terrestris Extract On Semen Quality And Serum Total Cholesterol Content In White Plymouth-Mini Cocks. Institute of Animal Science, Kostinbrod, Bulgaria. 24(3-4), p: 139-146.
• 24. Tuncer M.A, Yaymacı B, Satı, L, Çaylı, S., Acar, G., Altuğ, T., Demir, R. 2009. Influence of Tribulus terrestris extract on lipid profile and endothelial structure in developing atherosclerotic lesions in the aorta of rabbits on a highcholesterol diet. Acta Histochemica,. Volume: 111, Issue: 6, Pages: 488-500.
• 25. Amin, A, Lotfy M, Shafiullah M, And Adeghate, E. 2009. The Protective Effect of Tribulus terrestris in Diabetes. The New York Academy of Sciences. Volume: 1084, Pages: 391-401.
• 26. Duru M, Şahin A. 2015. Farklı Taşıyıcılarla Rasyona Eklenen Demir Dikeninin (Tribulus terrestris) Yumurtacı Tavuklarda Verim Ve Yumurta Kalitesi Üzerine Etkileri Journal of Food and Health Science. 1(2): 84-93 doi: 10.3153/JFHS15008
• 27. Botsoglou NA, Taitzoglou IA, Botsoglou E. et al. 2009. Effect of long-term dietary administration of oregano and rosemary on the antioxidant status of rat serum, liver, kidney and heart after carbon tetrachloride-induced oxidative stress. J Sci Food Agric. 89: 1397-406.
• 28. Al-Assaf AH. 2013. Preventive effect of corosolic acid on lipid profile against carbon tetrachloride induced hepatotoxic rats. Pak J Nut. 12: 748-52.
• 29. Rajesh MG, Latha MS. 2001. Hepatoprotection by Elephantopus scaber L. in CCl4-induced liver injury. Indian J Physiol Pharmacol. 45: 481-6.
• 30. Venukumar MR, Latha MS. 2002. Hepatoprotective effect of the methanolic extract Curculigo orchioides in CCl4 treated male rats. Indian J Pharmacol. 34: 269-75.
• 31. Santra A, Chowdhury A, Ghatak S, Biswas A, Dhali GK. 2007. Arsenic induces apoptosis in mouse liver is mitochondria dependent and is abrogated by N-acetylcysteine. Toxicol Appl Pharmacol; 220: 146-55.
• 32. Ahsan R, Islam KM, Musaddik A, Haque E. 2009. Hepatoprotective activity of methanol extract of some medicinal plants against carbon tetrachloride induced hepatotoxicity in Albino rats. Global J Pharm. 3: 116-22.
• 33. Feroz Khan Z, Asdaq SMB, Prasanna Kumar SR. 2009. Effects of few Indian medicinal herbs on carbon tetrachloride induced hepatic injury in animals. International J Pharm Tech Res. 1: 579-87