Fluorescent Copper Phosphate Nanoflowers: A Novel Toxicity Investigation Study Based On Tenebrio Molitor Linnaeus, 1758 (Coleoptera: Tenebrionidae) Larvae

Year 2020, Volume: 9 Issue: 3, 975 - 984, 26.09.2020

Ata Eskin , Cevahir Altınkaynak , Merve Türk Nalan Özdemir

https://doi.org/10.17798/bitlisfen.597483

Cited By: 3

Abstract

Organic-inorganic hybrid nanoflowers with commercialization
potential are the novel materials and are widely used in many experimental
applications, recently. However their potential toxicity values of these
nanoflowers are still unknown. In this study, fluorescent copper phosphate
nanoflowers (FCPnfs) were first synthesized to constructed via
organic-inorganic hybrid nanoflower synthesis method at Science Technology
Application and Research Center Laboratory of Nevsehir Haci Bektas Veli University
in 2018. The morphological features of FCPnfs (acridine orange amount, shape, size)
were analyzed. The results showed that different amount of acridine orange
caused different morphology and the organic component were homogeneously
distributed inside the nanoflowers. These materials were characterized by SEM,
EDX, XRD, and FTIR techniques. The toxic effects of the as-prepared FCPnfs were
investigated in Tenebrio molitor Linnaeus,
1758 (Coleoptera: Tenebrionidae) larvae compared to pure copper
phosphate nanoflowers (CPnfs) at Plant and Animal Production Department
Laboratory of Nevsehir Haci Bektas Veli University, Avanos Vocational School in
2018. Nanoflowers were force feed to larvae at the different doses of 0.001,
0.005, 0.010, 0.025, 0.050, 0.100 mg/10μl. According to probit assay, LC₅₀
and LC₉₉ values of FCPnfs were found 0.490 and 0.145 mg/10μl, respectively.
On the other hand, LC₅₀ and LC₉₉ values of CPnfs were
detected 0.066 and 0.172 mg/10μl, respectively. It was found that the insect
exhibited slightly more resistance to CPnfs than FCPnfs when compared to both
chemical toxic values. These new findings will be offer a new insight into how
to design new materials in industrial uses.

Keywords

Copper phosphate; Nanoflowers; Toxic effects.

Tenebrio molitor larvasında gerçekleştirilen yeni bir toksik araştırma: Floresan bakır fosfat nano çiçekler

Abstract

Son
zamanlarda birçok deneysel uygulamada yaygın olarak kullanılan
organik-inorganik hibrit nano çiçekler ticarileşme potansiyeline sahip yeni
malzemelerdir. Ancak bu nano çiçeklerin toksik etkileri hala bilinmemektedir.
Bu çalışmada, ilk defa organik-inorganik hibrit nano çiçek sentez metodu
kullanılarak floresan bakır fosfat nano çiçekler (FCPnfs) 2018 yılında Nevşehir
Hacı Bektaş Veli Üniversitesi, Bilim Teknoloji Araştırma ve Uygulama Merkezi
laboratuvarında sentezlendi. FCPnfs'in morfolojik özellikleri (akridin orange
miktarı, şekli, boyutu) analiz edildi. Sonuçlar, farklı miktarda akridin orange’ın
farklı morfolojiye neden olduğu ve organik bileşenin nano çiçeklerin içinde
homojen bir şekilde dağıldığını gösterdi. Sentezlenen malzemelerin
karakterizasyonları SEM, EDX, XRD ve FTIR teknikleri ile gerçekleştirildi. Model
organizma olarak seçilen Tenebrio molitor
Linnaeus, 1758 (Coleoptera: Tenebrionidae)
larvalarında sentezlenen FCPnfs'lerin toksik etkileri, saf bakır fosfat nano
çiçekleriyle (CPnfs) karşılaştırmalı olarak 2018 yılında Nevşehir Hacı Bektaş
Veli Üniversitesi, Avanos Meslek Yüksekokulu, Bitkisel ve Hayvansal Üretim
Laboratuvarı’nda incelendi. Larvalara nano çiçekler, 0.001, 0.005, 0.010,
0.025, 0.050, 0.100 mg/10µl olacak şekilde farklı dozlarda zorla besleme
yapıldı. Yapılan probit analizine göre, FCPnfs'in LC₅₀ değeri 0.490
mg/10µl iken LC₉₉ değeri 0.145 mg/10µl bulundu. Öte yandan,
CPnfs'nin LC₅₀ değeri 0.066 mg/10µl iken LC₉₉ değeri 0.172
mg/10µl bulundu. Elde edilen toksik veriler değerlendirildiğinde; Tenebrio molitor larvalarının CPnfs'ye
FCPnfs'den biraz daha fazla direnç gösterdiği bulundu. Bu bulgular, endüstriyel
kullanımlarda yeni malzemelerin nasıl tasarlanacağı hakkında yeni bir bakış
açısı kazandıracaktır.

Keywords

Bakır fosfat; Nano çiçekler; Toksik etki.

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