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Stachys macrantha (K.Koch) Stearn’ın Biyolojik Aktivitesinin Belirlenmesi

Year 2022, Volume: 11 Issue: 1, 156 - 163, 24.03.2022
https://doi.org/10.17798/bitlisfen.1002210

Abstract

Stachys, Lamiaceae familyasının en geniş yayılış gösteren cinslerinden birisidir. Bu cinse ait türler biyolojik aktiviteleri ve etnomedikal kullanımları ile bilinmektedir. Bu çalışmada, Stachys macrantha’nın toprak üstü kısımlarının farklı çözücülerle (etanol, metanol ve etil asetat) elde edilen ekstraktlarının toplam fenolik ve flavonoid içeriği ile antioksidan (ABTS, DPPH) ve enzim inhibisyon (α-amilaz, α-glukozidaz, bütirilkolinesteraz ve asetilkolinesteraz) aktiviteleri spektrofotometrik yöntemlerle araştırılmıştır. Ayrıca, fenolik bileşen analizi HPLC ile analiz edilmiştir. Bulgulara göre, metanol ekstraktının diğer ekstraktlara kıyasla biyolojik aktivitelerinin dikkat çekici olduğu bulunmuştur. Ekstraktın, toplam fenolik (45.57 mg GAE/g ekstrakt) ve flavonoid (54.48 mg QE/g ekstrakt) içeriği, antioksidan özellikleri [ABTS (49.99 mg TE/g ekstrakt), DPPH (23.62 mg TE/g ekstrakt)] ile enzim inhibisyon aktiviteleri [α-amilaz (159.35 mmol ACE/g ekstrakt), α-glukozidaz (8.78 mmol ACE/g ekstrakt), bütirilkolinesteraz (5.85 mmol ACE/g ekstrakt) ve asetilkolinesteraz (1.25mmol ACE/g ekstrakt)] belirlenmiştir. Metanol ekstraktının majör fenolik bileşenlerinin kaemferol (539.65 µg/g ekstrakt) ve kuersetin (271.65 µg/g ekstrakt) olduğu tespit edilmiştir. Elde edilen sonuçlara göre, bitkinin tespit edilen aktiviteleri sayesinde ilaç, gıda ve kozmetik endüstrilerinde kullanılma potansiyeli olduğu söylenebilir. Bununla birlikte, söz konusu verilerin daha ileri çalışmalarla desteklenmesi ve aktif bileşenlerin saflaştırılarak etkilerinin in vivo ortamlarda belirlenmesi gerekmektedir.

Supporting Institution

Gümüşhane Üniversitesi, Bilimsel Araştırma Projeleri Destekleme Birimi

Project Number

GÜBAP 16.F5119.02.01

References

  • [1] E. Akçiçek, “A new subspecies of Stachys cretica (section Eriostomum, Lamiaceae) from Turkey,” Turk J Botany, vol. 34, pp. 131-136, 2010.
  • [2] P.G. Rasgele and G. Dulger, “Chemical Compositions and Antimutagenic Effects of Ethanolic Extracts of Stachys thirkei and Stachys annua subsp. annua Using the Ames Assay,” Pharm. Chem. J., vol. 54, pp.1255-1262, 2021.
  • [3] M.B. Bahadori, B. Kirkan, and C. Sarikurkcu, “Phenolic ingredients and therapeutic potential of Stachys cretica subsp. smyrnaea. for the management of oxidative stress, Alzheimer’s disease, hyperglycemia and melasma,” Ind Crops Prod, vol.127, pp. 82-87, 2019.
  • [4] W. Elfalleh, B.Kirkan, and C. Sarikurkcu Antioxidant potential and phenolic composition of extracts from Stachys tmolea: An endemic plant from Turkey,” Ind Crops Prod, vol.127, pp. 212-216, 2019.
  • [5] I. Bouasla et al., “ Evaluation of solvent influence on phytochemical content and antioxidant activities of two Algerian endemic taxa: Stachys marrubiifolia Viv. and Lamium flexuosum Ten. (Lamiaceae),” Eur. J. Integr. Med., vol. 42, pp. 1-12, 2020.
  • [6] I. Çalis, A.A. Başaran, I. Saracoğlu, and O. Stcher, “Iridoid and phenylpropanoid glycosides from Stachys macrantha,” Phytochemistry, vol. 31, pp.167-169, 1992.
  • [7] R. Tundis, L. Peruzzi and F. Menichini, “Phytochemical and biological studies of Stachys species in relation to chemotaxonomy: A review,” Phytochemistry, vol. 102, pp. 7-39, 2014.
  • [8] C. Sarikurkcu, M.S. Kocak, M.C. Uren, M., Calapoglu, and A.S. Tepe, “Potential sources for the management global health problems and oxidative stress: Stachys byzantine and S. iberica subsp. iberica var. densipilosa,” Eur. J. Integr. Med., vol. 504, pp 1-7, 2015.
  • [9] V.B. Vundac, A.H. Brantner, and M. Plazibat, “Content of polyphenolic constituents and antioxidant activity of some Stachys taxa,”Food Chem., vol.104, pp. 1277-1281, 2007.
  • [10] S, Hazrati, K. Lotfi, M. Govahi and M.T. Ebadi, “A comparative study: Influence of various drying methods on essential oil components and biological properties of Stachys lavandulifolia,” Food Sci. Nutr., vol. 9, pp. 2612–2619, 2021.
  • [11] R. Akula and G.A. Ravishankar, “Influence of abiotic stress signals on secondary metabolites in plants,” Plant Signal. Behav., vol. 6, no. 11, pp. 1720-1731, 2011.
  • [12] M.V. Sharifi-Rad et al., “Lifestyle, Oxidative Stress, and Antioxidants: Back and Forth in the Pathophysiology of Chronic Diseases,” Front. Physiol., vol. 11, pp. 694, 2020.
  • [13] T. Acet, “Chemical profile, antioxidant, antimicrobial, and enzyme inhibition activities of Pilosella hoppeana subsp. cilicica,” Plant Biosyst., 2021, DOI: 10.1080/11263504.2021.1894257
  • [14] K., Özcan and T. Acet, “In vitro antioxidant and antimicrobial activities of the five different solvent extracts of Centaurea pulcherrima var. freynii from Turkey,” Fresenius Environ. Bull., vol.27, no.6, pp. 4047-4051, 2018.
  • [15] T. Acet, K. Özcan, and G. Zengin, “An assessment of phenolic profiles, fatty acid compositions, and biological activities of two Helichrysum species: H. plicatum and H. chionophilum,” J. Food Biochem., vol. 44, pp. e13128, 2020.
  • [16] B. Kirkan, “Antioxidant potential, enzyme inhibition activity, and phenolic profile of extracts from Stachys cretica subsp. vacillans,” Ind Crops Prod, vol. 140, pp. 111639, 2019.
  • [17] B. Tepe, S. Degerli, S.Arslan, E. Malatyali, and C. Sarikurkcu, “Determination of chemical profile, antioxidant, DNA damage protection and antiamoebic activities of Teucrium polium and Stachys iberica,” Fitoterapia, vol. 82, no. 2, pp. 237-246, 2011.
  • [18] M. Imran et al., “Kaempferol: A Key Emphasis to Its Anticancer Potential,” Molecules, vol. 24, no.12, pp. 2277, 2019.
  • [19] J.M. Calderon-Montano, E. Burgos-Moron, C. Perez-Guerrero, and M. Lopez-Lazaro, “A review on the dietary flavonoid kaempferol,” Mini Rev Med Chem, vol. 11, pp. 298-344, 2011.
  • [20] D. Xu, M.J. Hu, Y.Q., Wang, and Y.L. Cui, “Antioxidant Activities of Quercetin and Its Complexes for Medicinal Application” Molecules, vol. 24, no. 6, pp. 1123, 2019.
  • [21] M.B., Bahadori, G. Zengin, L. Dinparast, and M. Eskandani, The health benefits of three Hedgenettle herbal teas (Stachys byzantina, Stachys inflata, and Stachys lavandulifolia) - profiling phenolic and antioxidant activities,” Eur. J. Integr. Med., vol. 36, pp. 101134, 2020.
  • [22] F. Olazarán-Santibañez et al., “Antioxidant and Antiproliferative Activity of the Ethanolic Extract of Equisetum myriochaetum and Molecular Docking of Its Main Metabolites (Apigenin, Kaempferol, and Quercetin) on β-Tubulin,” Molecules, vol. 26, pp. 443, 2021.
  • [23] M.A. Benabderrahim, C. Sarikurkcu, W. Elfalleh, M.S. Ozer, and O. Ceylan, “Phenolic composition and biological activities of Turkish endemic plant: Stachys cretica subsp. kutahyensis,” S. Afr. J. Bot., vol. 138, pp. 124-128, 2021.
  • [24] Y. Bian, “Kaempferol reduces obesity, prevents intestinal inflammation, and modulates gut microbiota in high-fat diet mice,” J. Nutr. Biochem, vol. 99, pp. 108840, 2022.

Determining of Biological Activity of Stachys macrantha (K.Koch) Stearn

Year 2022, Volume: 11 Issue: 1, 156 - 163, 24.03.2022
https://doi.org/10.17798/bitlisfen.1002210

Abstract

Project Number

GÜBAP 16.F5119.02.01

References

  • [1] E. Akçiçek, “A new subspecies of Stachys cretica (section Eriostomum, Lamiaceae) from Turkey,” Turk J Botany, vol. 34, pp. 131-136, 2010.
  • [2] P.G. Rasgele and G. Dulger, “Chemical Compositions and Antimutagenic Effects of Ethanolic Extracts of Stachys thirkei and Stachys annua subsp. annua Using the Ames Assay,” Pharm. Chem. J., vol. 54, pp.1255-1262, 2021.
  • [3] M.B. Bahadori, B. Kirkan, and C. Sarikurkcu, “Phenolic ingredients and therapeutic potential of Stachys cretica subsp. smyrnaea. for the management of oxidative stress, Alzheimer’s disease, hyperglycemia and melasma,” Ind Crops Prod, vol.127, pp. 82-87, 2019.
  • [4] W. Elfalleh, B.Kirkan, and C. Sarikurkcu Antioxidant potential and phenolic composition of extracts from Stachys tmolea: An endemic plant from Turkey,” Ind Crops Prod, vol.127, pp. 212-216, 2019.
  • [5] I. Bouasla et al., “ Evaluation of solvent influence on phytochemical content and antioxidant activities of two Algerian endemic taxa: Stachys marrubiifolia Viv. and Lamium flexuosum Ten. (Lamiaceae),” Eur. J. Integr. Med., vol. 42, pp. 1-12, 2020.
  • [6] I. Çalis, A.A. Başaran, I. Saracoğlu, and O. Stcher, “Iridoid and phenylpropanoid glycosides from Stachys macrantha,” Phytochemistry, vol. 31, pp.167-169, 1992.
  • [7] R. Tundis, L. Peruzzi and F. Menichini, “Phytochemical and biological studies of Stachys species in relation to chemotaxonomy: A review,” Phytochemistry, vol. 102, pp. 7-39, 2014.
  • [8] C. Sarikurkcu, M.S. Kocak, M.C. Uren, M., Calapoglu, and A.S. Tepe, “Potential sources for the management global health problems and oxidative stress: Stachys byzantine and S. iberica subsp. iberica var. densipilosa,” Eur. J. Integr. Med., vol. 504, pp 1-7, 2015.
  • [9] V.B. Vundac, A.H. Brantner, and M. Plazibat, “Content of polyphenolic constituents and antioxidant activity of some Stachys taxa,”Food Chem., vol.104, pp. 1277-1281, 2007.
  • [10] S, Hazrati, K. Lotfi, M. Govahi and M.T. Ebadi, “A comparative study: Influence of various drying methods on essential oil components and biological properties of Stachys lavandulifolia,” Food Sci. Nutr., vol. 9, pp. 2612–2619, 2021.
  • [11] R. Akula and G.A. Ravishankar, “Influence of abiotic stress signals on secondary metabolites in plants,” Plant Signal. Behav., vol. 6, no. 11, pp. 1720-1731, 2011.
  • [12] M.V. Sharifi-Rad et al., “Lifestyle, Oxidative Stress, and Antioxidants: Back and Forth in the Pathophysiology of Chronic Diseases,” Front. Physiol., vol. 11, pp. 694, 2020.
  • [13] T. Acet, “Chemical profile, antioxidant, antimicrobial, and enzyme inhibition activities of Pilosella hoppeana subsp. cilicica,” Plant Biosyst., 2021, DOI: 10.1080/11263504.2021.1894257
  • [14] K., Özcan and T. Acet, “In vitro antioxidant and antimicrobial activities of the five different solvent extracts of Centaurea pulcherrima var. freynii from Turkey,” Fresenius Environ. Bull., vol.27, no.6, pp. 4047-4051, 2018.
  • [15] T. Acet, K. Özcan, and G. Zengin, “An assessment of phenolic profiles, fatty acid compositions, and biological activities of two Helichrysum species: H. plicatum and H. chionophilum,” J. Food Biochem., vol. 44, pp. e13128, 2020.
  • [16] B. Kirkan, “Antioxidant potential, enzyme inhibition activity, and phenolic profile of extracts from Stachys cretica subsp. vacillans,” Ind Crops Prod, vol. 140, pp. 111639, 2019.
  • [17] B. Tepe, S. Degerli, S.Arslan, E. Malatyali, and C. Sarikurkcu, “Determination of chemical profile, antioxidant, DNA damage protection and antiamoebic activities of Teucrium polium and Stachys iberica,” Fitoterapia, vol. 82, no. 2, pp. 237-246, 2011.
  • [18] M. Imran et al., “Kaempferol: A Key Emphasis to Its Anticancer Potential,” Molecules, vol. 24, no.12, pp. 2277, 2019.
  • [19] J.M. Calderon-Montano, E. Burgos-Moron, C. Perez-Guerrero, and M. Lopez-Lazaro, “A review on the dietary flavonoid kaempferol,” Mini Rev Med Chem, vol. 11, pp. 298-344, 2011.
  • [20] D. Xu, M.J. Hu, Y.Q., Wang, and Y.L. Cui, “Antioxidant Activities of Quercetin and Its Complexes for Medicinal Application” Molecules, vol. 24, no. 6, pp. 1123, 2019.
  • [21] M.B., Bahadori, G. Zengin, L. Dinparast, and M. Eskandani, The health benefits of three Hedgenettle herbal teas (Stachys byzantina, Stachys inflata, and Stachys lavandulifolia) - profiling phenolic and antioxidant activities,” Eur. J. Integr. Med., vol. 36, pp. 101134, 2020.
  • [22] F. Olazarán-Santibañez et al., “Antioxidant and Antiproliferative Activity of the Ethanolic Extract of Equisetum myriochaetum and Molecular Docking of Its Main Metabolites (Apigenin, Kaempferol, and Quercetin) on β-Tubulin,” Molecules, vol. 26, pp. 443, 2021.
  • [23] M.A. Benabderrahim, C. Sarikurkcu, W. Elfalleh, M.S. Ozer, and O. Ceylan, “Phenolic composition and biological activities of Turkish endemic plant: Stachys cretica subsp. kutahyensis,” S. Afr. J. Bot., vol. 138, pp. 124-128, 2021.
  • [24] Y. Bian, “Kaempferol reduces obesity, prevents intestinal inflammation, and modulates gut microbiota in high-fat diet mice,” J. Nutr. Biochem, vol. 99, pp. 108840, 2022.
There are 24 citations in total.

Details

Primary Language Turkish
Subjects Engineering
Journal Section Araştırma Makalesi
Authors

Kadriye Özcan 0000-0002-4913-6035

Tuba Acet 0000-0002-0981-9413

Project Number GÜBAP 16.F5119.02.01
Publication Date March 24, 2022
Submission Date September 29, 2021
Acceptance Date November 25, 2021
Published in Issue Year 2022 Volume: 11 Issue: 1

Cite

IEEE K. Özcan and T. Acet, “Stachys macrantha (K.Koch) Stearn’ın Biyolojik Aktivitesinin Belirlenmesi”, Bitlis Eren Üniversitesi Fen Bilimleri Dergisi, vol. 11, no. 1, pp. 156–163, 2022, doi: 10.17798/bitlisfen.1002210.

Bitlis Eren University
Journal of Science Editor
Bitlis Eren University Graduate Institute
Bes Minare Mah. Ahmet Eren Bulvari, Merkez Kampus, 13000 BITLIS