Healing Effect of Ascorbic Acid against Genetic and Epigenetic Changes Caused by Pendimethalin in Wheat

Year 2023, Volume: 12 Issue: 4, 978 - 984, 28.12.2023

Nalan Yıldırım Doğan , Muhammed Semih Dartar

https://doi.org/10.17798/bitlisfen.1305017

Abstract

Because of the increasing need for agricultural products in the world, the use of pesticides, which are used to increase yield, is increasing day by day. Herbicides constitute a large part of the total amount of pesticides used, such as 20%. It is known that herbicides have toxic effects and irreversibly disrupt DNA and gene expression. Pendimethalin is a widely used herbicide against weeds in the production of grains, legumes, and vegetables. Ascorbic acid has an antioxidant effect. Molecular markers are frequently used to determine genotoxic and mutagenic effects at the DNA level. It was aimed to determine the curative effect of ascorbic acid on the negative effects of pendimethalin. IRAP and ISSR molecular markers were used. It was found that the GTS ratio decreased as a result of increasing the dose of pendimethalin applied in wheat, resulting in DNA damage and the positive effect of applied ascorbic acid on DNA damage.

Keywords

Genotoxicity, Herbicide, IRAP, ISSR, Triticum aestivum, Vitamin C

Project Number

Proje Numara: FYL-2022-815

References

• [1] G. Beker Akbulut, “Atrazin ve asetoklor herbisitlerinin Zea mays L. (mısır) ve Pisum sativum L.(bezelye) bitkilerinde biyokimyasal ve fizyolojik parametreler üzerine etkileri,” İnönü Üniversitesi Fen Bilimleri Enstitüsü, 2008.
• [2] P. Gupta, and S. K. Verma, “Evaluation of genotoxicity induced by herbicide pendimethalin in fresh water fish Clarias batrachus (linn.) and possible role of oxidative stress in induced DNA damage, ”Drug and Chemical Toxicology, vol. 45, no. 2, pp. 750–759, 2020.
• [3] S. Sciacca and G. O. Conti. "Mutagens and carcinogens in drinking water," Mediterranean Journal of Nutrition and Metabolism, vol. 2, pp. 157–162, 2009.
• [4] G. Andreotti, L.E.B. Freeman, L. Hou, J. Coble, J. Rusiecki, J.A Hoppin, D.T. Silverman and M.C. R. Alavanja, "Agricultural pesticide use and pancreatic cancer risk in the Agricultural Health Study Cohort, " International Journal of Cancer, vol. 124, no. 10, pp. 2495-2500, 2009.
• [5] S. S. Sternberg, "The carcinogenesis, mutagenesis and teratogenesis of insecticides. Review of studies in animals and man," Pharmacology & Therapeutics, vol. 6, no. 1, pp. 147-166, 1979. doi.org/10.1016/0163-7258(79)90059-7.
• [6] N. S. Hammok and F. A. Al-mandeel, "Effect of Different application methods for pendimethalin herbicide on growth and productivity of green pea plant (Pisum sativum L.)," Current Applied Scince and Technology, vol. 2, no.3, pp. 528-536, 2020.
• [7] Ü. Ündeğer, M. Schlumpf and W. Lichtensteiger, "Effect of the herbicide pendimethalin on rat uterine weight and gene expression and in silico receptor binding analysis." Food and Chemical Toxicology, vol. 48, no. 2, pp. 502-508, 2010, doi.org/10.1016/j.fct.2009.11.001.
• [8] S. Verma and A. Srivastava, "Morphotoxicity and cytogenotoxicity of pendimethalin in the test plant Allium cepa L.-A biomarker based study," Chemosphere, vol. 206, pp. 248-254, 2018, doi.org/10.1016/j.chemosphere.2018.04.177.
• [9] N. Singh and A. Srivastava, "Biomonitoring of genotoxic effect of glyphosate and pendimethalin in Vigna mungo populations." Cytologia, vol. 79, no. 2, pp. 173-180, 2014.
• [10] Y. Lv, Y. Li, X. Liu and K. Xu, "Toxicity and tissue accumulation characteristics of the herbicide pendimethalin in ginger (Zingiber officinale Roscoe)," Environmental Science and Pollution Research, vol. 29, pp.25263–25275, 2022.
• [11] M. I. Ahmad, M. F. Zafeer, M. Javed and M. Ahmad, "Pendimethalin-induced oxidative stress, DNA damage and activation of anti-inflammatory and apoptotic markers in male rats." Scientific Reports, vol. 8, no. 1, pp. 17139, 2018.
• [12] S. M. Ansari, Q. Saquib, S. M. Attia, E. M. Abdel-Salam, H. A. Alwathnani, M. Faisal and J. Musarrat, "Pendimethalin induces oxidative stress, DNA damage, and mitochondrial dysfunction to trigger apoptosis in human lymphocytes and rat bone-marrow cells," Histochemistry and Cell Biology, vol. 149, no. 2, pp. 127-141. 2018, doi.org/10.1007/s00418-017-1622-0.
• [13] H. Park, J. Y. Lee, W. Lim and G. Song, "Assessment of the in vivo genotoxicity of pendimethalin via mitochondrial bioenergetics and transcriptional profiles during embryogenesis in zebrafish: Implication of electron transport chain activity and developmental defects." Journal of Hazardous Materials, vol. 411, 125153, 2021.
• [14] B. D. Dimitrov, P. G. Gadeva, D. K. Benova and M. V. Bineva, "Comparative genotoxicity of the herbicides Roundup, Stomp and Reglone in plant and mammalian test systems." Mutagenesis, vol. 21, no. 6, pp. 375-382, 2006.
• [15] E. Akbulut, "Pendimethalin ve salisilik asit uygulamalarının Carthamus tinctorius l. cv. “remzibey” yağ asidi desatüraz genlerinin anlatımı üzerine etkisi," Journal of the Institute of Science and Technology, vol. 10, no. 4, pp. 2915-2925, 2020.
• [16] B. Halliwell, "Vitamin C and genomic stability," Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, vol. 475(1-2), pp. 29-35. 2001.
• [17] K. Premkumar and C. L. Bowlus, "Ascorbic acid reduces the frequency of iron induced micronuclei in bone marrow cells of mice." Mutation Research/Genetic Toxicology and Environmental Mutagenesis, vol. 542, pp. 99-103, 2003.
• [18] B. Kaya, A. Creus, A. Velázquez, A. Yanikoğlu and R. Marcos, "Genotoxicity is modulated by ascorbic acid: Studies using the wing spot test in Drosophila, Mutation Research/Genetic Toxicology and Environmental Mutagenesis, vol. 520, pp. 93-101, 2002, doi.org/10.1016/S1383-5718(02)00173-0.
• [19] P. Banerjee, S. S. Bhattacharyya, N. Bhattacharjee, S. Pathak, N. Boujedaini, P. Belon and A. R. Khuda-Bukhsh, "Ascorbic acid combats arsenic-induced oxidative stress in mice liver." Ecotoxicology and Environmental Safety, vol. 72, pp. 639-649, 2009, doi.org/10.1016/j.ecoenv.2008.07.005.
• [20] I. C. Ozturk, F. Ozturk, M. Gul, B. Ates and A. Cetin, "Protective effects of ascorbic acid on hepatotoxicity and oxidative stress caused by carbon tetrachloride in the liver of Wistar rats. " Cell Biochemistry and Function: Cellular biochemistry and its modulation by active agents or disease, vol. 27, no. 5, pp. 309-315, 2009, doi.org/10.1002/cbf.1575.
• [21] S. Verma, A.Srivastava, "Morphotoxicity and cytogenotoxicity of pendimethalin in the test plant Allium cepa L. - A biomarker based study." Chemosphere, vo. 206, pp. 248-254, 2018.
• [22] H. Barakat, "Interactive effects of salinity and certain vitamins on gene expression and cell division," Internatıonal Journal of Agrıculture & Bıology, vol. 5, no. 3, pp. 219–225, 2003.
• [23] M. A. Saghai-Maroof, K. M. Soliman, R. A. Jorgensen and R. W. Allard, "Ribosomal DNAsepacer-length polymorphism in barley: mendelian inheritance, chromosomal location, and population dynamics," Proceedings of The National Academy Sciences, vol. 81, pp. 8014-8019, 1984.
• [24] H. Bulut, N. Y. Doğan and M. Korkmaz, "Tıbbi ve aromatik bitki olarak kullanılan Tanacetum sp. (pire otu) türlerinin genetik benzerliğinin moleküler yöntemler ile belirlenmesi." Manas Journal of Agriculture Veterinary and Life Sciences, vol. 9, no.1, pp. 22-29, 2019.
• [25] F. A Atienzar, M. Conradi, A. J. Evenden, A. N. Jha and M. H. Depledge, "Qualitative assessment of genotoxicity using random amplified polymorphic DNA: comparison of genomic template stability with key fitness parameters in Daphnia magna exposed to benzo pyrene." Enviromental Toxicology Chemistry, vol. 18, pp. 2275-2282, 1999. doi.org/10.1002/etc.5620181023.
• [26] P. Gupta and S.K. Verma, "Evaluation of genotoxicity induced by herbicide pendimethalin in fresh water fish Clarias batrachus (linn.) and possible role of oxidative stress in induced DNA damage," Drug Chemical Toxicology, vol.45, no. 2, pp. 750-759, 2020, doi.org/10.1080/01480545.2020.1774603.
• [27] S. Patel, M. Bajpayee, A. K. Pandey, D. Parmar and A. Dhawan, "In vitro induction of cytotoxicity and DNA strand breaks in CHO cells exposed to cypermethrin, pendimethalin and dichlorvos," Toxicology In Vitro, vol. 21, no. 8, pp. 1409-1418, 2007.
• [28] M. C. Alavanja, M. Dosemeci, C. Samanic, J. Lubin, C. F. Lynch, C. Knott and A. Blair, "Pesticides and lung cancer risk in the agricultural health study cohort," American Journal Of Epidemiology, vol. 160, no. 9, pp. 876-885, 2004.
• [29] M. Arici, M. Abudayyak, T. Boran, G. Özhan, "Does pendimethalin develop in pancreatic cancer induced inflammation? " Chemosphere, vol. 252, pp. 126644, 2020.
• [30] B. Taşdemir, R. Liman, S. Gökçe, E. Amaç, İ. H. Ciğerci and S. E. Korcan, "Cytogenotoxic and forced degradation studies of pendimethalin using root growth, comet assay and LC-MS/MS, " Annals of Clinical and Analytical Medicine, vol. 12, no. 1, pp. 96-100, 2021.
• [31] N. Promkaew, P. Soontornchainaksaeng, S. Jampatong and P. Rojanavipart, "Toxicity and genotoxicity of pendimethalin in maize and onion" Agriculture and Natural Resources, vol. 44, no. 6, pp. 1010-1015, 2010.
• [32] A. M. Anghel, A. G. G. Sîrbu, S. Ostan, C. Ianăş, and M. Corneanu, "The assessment of pendimethalin cytotoxity by Allium assay," Research Journal of Agricultural Science, vol. 51, no. 2, pp. 3-10, 2019.