Sıçanlarda Parasetamol Kaynaklı Testis Toksisitesinde Terapötik Bir Ajan Olarak Bor: Biyokimyasal Bir Bakış Açısı

Abstract

Çalışmada, sıçan testis dokusunda parasetamol (PR) kaynaklı toksisiteye karşı borun koruyucu etkileri çeşitli biyokimyasal parametreler kullanılarak araştırıldı. Sıçanlar beş gruba ayrıldı ve altı gün boyunca oral yoldan 50 ve 100 mg/kg bor (sodyum pentaborat) verildi, ardından toksisiteyi oluşturmak için tek doz 1 g/kg parasetamol verildi. Testis dokuları ELISA kullanılarak değerlendirildi ve süperoksit dismutaz (SOD), katalaz (CAT), glutatyon peroksidaz (GPx), sistein-aspartik asit proteaz (Kaspaz-3), malondialdehit (MDA), indirgenmiş glutatyon (GSH), tümör nekroz faktörü-α (TNF-α) ve interlökin-1 beta (IL-1β) düzeyleri değerlendirildi. PR grubunda SOD, CAT, GPx aktiviteleri ve GSH düzeylerinde anlamlı azalma, MDA düzeylerinde ise artış gözlendi. Bor (B) tedavisinin parasetamol kaynaklı testis toksisitesinde antioksidan seviyelerini artırırken lipid peroksidasyonunu, inflamasyonu ve apoptozis belirteçlerini azalttığı bulundu (p<0,001). Çalışma, borun parasetamol kaynaklı testis hasarını hafifletmede etkili olabileceği sonucuna vardı.

Keywords

• bor
• inflamasyon
• oksidatif stres
• parasetamol toksisitesi
• testiküler hasar

Boron as a protective agent in reducing paracetamol-induced testicular toxicity in rats: A biochemical perspective

Abstract

In the study, the protective effects of boron against paracetamol (PR)-induced toxicity in rat testicular tissue were investigated using various biochemical parameters. Rats were categorized into five groups and administered 50 and 100 mg/kg of boron (sodium pentaborate) orally for six days, followed by a single dosage of 1 g/kg of paracetamol to induce toxicity. Testicular tissues were assessed using ELISA and levels of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), cysteine-aspartic acid protease (Caspase-3), malondialdehyde (MDA), reduced glutathione (GSH), tumor necrosis factor-α (TNF-α), and interleukin-1 beta (IL-1β) were assessed. A significant decrease in SOD, CAT, GPx activities and GSH levels and an increase in MDA levels were observed in the PR group. Boron (B) treatment was found to increase antioxidant levels while decreasing lipid peroxidation, inflammation and apoptosis markers in paracetamol-induced testicular toxicity (p<0.001). The study concluded that boron may be effective in alleviating paracetamol-induced testicular damage.

Keywords

• boron
• inflammation
• oxidative stress
• paracetamol toxicity
• testicular damage

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