İNFERTİL BİREYLERDE SEMEN HACİM VE SPERM UZUNLUK ÖLÇÜMLERİNİN EZRİN VE N-KADERİN PROTEİNLERİ İLE İLİSKİLENDİRİLMESİ Association of Semen Volume and Sperm Length Measurements with Ezrin and N-Cadherin Proteins in Infertile Men
Abstract
ÖZET
Amaç: İnfertil bireylere ait sperm hacim/uzunluk oranları ve spermiyogenezin fizyolojik sürecinde aktin
bağlayıcı protein olan ve bir çapraz bağlayıcı olarak kortikal hücre iskeleti ile plazma membran proteinleri
arasında bulunan ezrin, ile bir transmembran glikoproteinlerin olan N-kaderin kolakizasyonlarının araştırılması
gerçekleştirilmiştir.
Gereç ve Yöntemler: N= 18 infertil bireyde hacim/uzunluk oranları ve sperm sayımı gerçekleştirilen semen
önekleri sperm yayma preparatları Kruger Kriterlerine göre mikroskop altında incelendi. Her grup için
sperm uzunluk ölçümleri gerçekleştirildi, immünofloresan boyama ile ezrin ve N-kaderin lokalizasyon ve
ekspresyonlarıdeğerlendirildi.
Bulgular: Sperm uzunluklarının hacim yoğunluklarına oranla azaldığı ve sperm uzunluğunun sperm sayısı ile
arasında anlamlı korelasyon olduğu tespit edildi. Sperm kuyruk uzunluğu anomalisinin infertilite üzerinde
etkin bir durum olduğu ancak ezrin ve N-kaderin lokalizasyon ve ekspresyonlarında control grubuna göre
anlamlı fark olmadığı tespit edildi.
Sonuç: Sperm sayısı/hacim ve sperm uzunluk oranlarının infertil bireylerde spermiyogenezin fizyolojik
sürecine bağlı olarak gelişebileceği ilk kez bu proje ile gösterilmiş olup, sperm morfolojik özelliklerinin semen
hacmi ile ilişkili olabileceği ve infertil bireylerde belirli fonksiyonel proteinlerin tespitinin gerçekleşmesine
yönelik çalışmaların gerekliliği vurgulanmıştır.
Anahtar Kelimeler: Erkek infertilitesi; Semen analizi; Sperm uzunluğu; Ezrin, N-kaderin
ABSTRACT
Aim: The aim of this study was to investigate the sperm volume/lenght ratios of infertility individuals and
N-cadherin co-localizations, which are the actin binding protein in the process of spermiogenesis, and ezrin,
which are between the cortical cell skeleton and plasma membrane proteins, as a cross-linker.
Material- Method: Semen samples taken from infertilite individuals (N=18) examined by microscopy
after staining and fixation. The volume/length ratio was performed. Sperm morphology was examined as
sperm smear preparations according to Kruger Criteria. Sperm length measurements were performed for
localization and expression parameters of ezrin and N-cadherin were examined by immunofluorescence
staining.
Results: The sperm length of the patients with similar morphological criteria decreased with respect to
volume and there was a significant correlation between sperm length and sperm count. It was found that
sperm tail length anomaly was effective on infertility, but there was no significant difference in localization
and expression of ezrin and N-cadherin compared to control group.
Conclusion: It has been shown for the first time that sperm count/volume and sperm length ratios may
develop depending on the process of spermiogenesis in infertile individuals, it is emphasized that sperm
morphological features may be related to semen volumes and necessity of studies fort he detection of
certain functional proteins in infertile individuals is emphasized.
Key Words: Male infertility; Semen analysis; Sperm length; Ezrin; N-cadherin
References
- 1. Erdemir, F., F. Fırat, and Y. Gençten, Sperm morfolojisinin değerlendirilmesi ve klinik önemi. Turk Urol Sem, 2011. 2: p. 11-7. 2. Cheng, C.Y. and D.D. Mruk, The blood-testis barrier and its implications for male contraception. Pharmacological reviews, 2012. 64(1): p. 16-64. 3. Wang, L., et al., The role of ezrin-associated protein network in human sperm capacitation. Asian journal of andrology, 2010. 12(5): p. 667. 4. Marín-Briggiler, C., et al., Neural cadherin is expressed in human gametes and participates in sperm–oocyte interaction events. International journal of andrology, 2010. 33(1): p. e228-e239. 5. Organization, W.H., WHO laboratory manual for the examination and processing of human semen. 2010. 6. Cheng, C.Y. and D.D. Mruk, Actin binding proteins and spermiogenesis: Some unexpected findings. Spermatogenesis, 2011. 1(2): p. 99-104. 7. Ben, M.R., et al., Genetic aspects of male infertility: From bench to clinic. Gynecologie, obstetrique, fertilite & senologie, 2019. 47(1): p. 54-62. 8. Lu, J.-C., Y.-F. Huang, and N.-Q. Lü , WHO Laboratory Manual for the Examination and Processing of Human Semen: its applicability to andrology laboratories in China. Zhonghua nan ke xue= National journal of andrology, 2010. 16(10): p. 867-871. 9. Nakagawa, S. and M. Takeichi, Neural crest emigration from the neural tube depends on regulated cadherin expression. Development, 1998. 125(15): p. 2963-2971. 10. O'shaughnessy, P., et al., Effect of FSH on testicular morphology and spermatogenesis in gonadotrophindeficient hypogonadal mice lacking androgen receptors. Reproduction (Cambridge, England), 2010. 139(1): p. 177.
Abstract
References
- 1. Erdemir, F., F. Fırat, and Y. Gençten, Sperm morfolojisinin değerlendirilmesi ve klinik önemi. Turk Urol Sem, 2011. 2: p. 11-7. 2. Cheng, C.Y. and D.D. Mruk, The blood-testis barrier and its implications for male contraception. Pharmacological reviews, 2012. 64(1): p. 16-64. 3. Wang, L., et al., The role of ezrin-associated protein network in human sperm capacitation. Asian journal of andrology, 2010. 12(5): p. 667. 4. Marín-Briggiler, C., et al., Neural cadherin is expressed in human gametes and participates in sperm–oocyte interaction events. International journal of andrology, 2010. 33(1): p. e228-e239. 5. Organization, W.H., WHO laboratory manual for the examination and processing of human semen. 2010. 6. Cheng, C.Y. and D.D. Mruk, Actin binding proteins and spermiogenesis: Some unexpected findings. Spermatogenesis, 2011. 1(2): p. 99-104. 7. Ben, M.R., et al., Genetic aspects of male infertility: From bench to clinic. Gynecologie, obstetrique, fertilite & senologie, 2019. 47(1): p. 54-62. 8. Lu, J.-C., Y.-F. Huang, and N.-Q. Lü , WHO Laboratory Manual for the Examination and Processing of Human Semen: its applicability to andrology laboratories in China. Zhonghua nan ke xue= National journal of andrology, 2010. 16(10): p. 867-871. 9. Nakagawa, S. and M. Takeichi, Neural crest emigration from the neural tube depends on regulated cadherin expression. Development, 1998. 125(15): p. 2963-2971. 10. O'shaughnessy, P., et al., Effect of FSH on testicular morphology and spermatogenesis in gonadotrophindeficient hypogonadal mice lacking androgen receptors. Reproduction (Cambridge, England), 2010. 139(1): p. 177.
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Health Care Administration |
| Journal Section | Original Research |
| Authors | |
| Publication Date | March 25, 2020 |
| Published in Issue | Year 2020 Volume: 10 Issue: 1 |