ANTRENMANLI BİREYLERDE AEROBİK EGZERSİZ İNDÜKLÜ METABOLİK VE KARDİYAK STRESİN İRİSİN SEVİYELERİNE ETKİLERİ Effects of Aerobic Exercise Induced Metabolic and Cardiac Stress on Irisin Levels in Trained Subjects
Abstract
ÖZET
Amaç: Egzersizin vücudun oksidatif stres seviyesini ve metabolik aktiviteyi hızlandırdığı bilinmektedir. İrisin,
egzersizle indüklenen oksidatif stres ve enerji regülasyonunda rol alan önemli bir miyokindir. Bu çalışmadaki
amacımız antrenmanlı erkeklerde aerobik egzersiz ile indüklenen kardiyak ve iskelet kası stresinin belirlenmesinde
malondialdehyde (MDA), asymmetric dimethylarginine (ADMA) ve irisin düzeylerini belirlemektir.
Yöntem: Çalışmamızda toplamda 25 erkek deneğe aerobik koşu egzersizi uygulandı, yaklaşık 45 dakikalık
anaerobik eşik seviyesine denk geldi. Egzersizden hemen önce ve sonra venöz kan örnekleri alındı. Serum
irisin seviyeleri ölçümünde ELISA, ADMA ve MDA ölçümünde HPLC kullanıldı.
Bulgular: Aerobik egzersiz ADMA (0.417±0.05 μmol/L vs 0.537±0.05 μmol/L), MDA (0.769±0.02 μmol/L vs
1.012±0.04 μmol/L) ve irisin (252±4 ng/ml vs 299±4 ng/ml) düzeylerinde sistematik artışa neden oldu. Ayrıca
egzersiz boyunca ADMA ve irisin düzeyleri arasında anlamlı bir korelasyon gözlendi.
Sonuç: Egzersiz ile indüklenen kardiyak kalp stresi ile artmış ADMA ve irisin düzeyleri arasında bir ilişki gözlemlendi.
Böylece irisin, kardiyovasküler bozokluğu olan hastalar için koruyucu madde olarak düşünülebilir.
Anahtar kelimeler: Aerobik egzersiz; Anaerobik eşik; MDA; ADMA ; Irisin
ABSTRACT
Objective: Exercise is known to be accelerates metabolic activity and increases oxidative stress levels of the
body. Irisin is an exercise induced myokine which plays crucial role in energy regulation and oxidative stress.
We aimed to evaluate effects of aerobic exercise induced cardiac and skeletal muscle stress as determined
on malondialdehyde (MDA) and asymmetric dimethylarginine (ADMA) ADMA on irisin levels in trained male
subjects.
Material and Methods: Total of 25 male performed aerobic running exercise corresponded to their anaerobic
threshold levels approximately 45 min. Venous blood samples were taken before and immediately after
exercise. Serum irisin levels were determined using enzyme-linked immunoassay (ELISA) method. ADMA
and MDA measured by using high performance liquid chromatography (HPLC).
Results: Aerobic exercise led to systematic increase in ADMA (0.417±0.05 μmol/L vs 0.537±0.05 μmol/L),
MDA (0.769±0.02 μmol/L vs 1.012±0.04 μmol/L) and irisin (252±4 ng/ml vs 299±4 ng/ml). In addition, we
have observed significant correlation between increased ADMA and irisin levels during exercise
Conclusion: We observed close relationships between exercises induced cardiac muscle stress as determined
from increased ADMA levels and increased irisin levels. Thus, irisin may be considered as a protective
agent for the patients with cardiovascular system impairments.
Keywords: Aerobic exercise; Anaerobic threshold; MDA; ADMA; Irisin
References
- 1. Wasserman K, Hansen JE, Sue DY, Stringer W, Sietsema KE, Sun XG, et al. Principles of Exercise Testing and Interpretation: Including Pathophysiology and Clinical Applications, Lippincott Williams & Wilkins, Philadelphia, PA, USA, 5th edition, 2012. 2. Ozcelik O, Ozkan Y, Algul S, Colak R. Beneficial effects of training at the anaerobic threshold in addition to pharmacotherapy on weight loss, body composition, and exercise performance in women with obesity. Patient Preference and Adherence. 2015;9:999-1004. 3. Ward SA, Whipp BJ. Kinetics of the ventilatory and metabolic responses to moderate-intensity exercise in humans following prior exercise-induced metabolic acidaemia. Advences in Experimental Medicine and Biology. 2010;669:323-6. 4. Boström P, Wu J, Jedrychowski MP, Korde A, Ye L, Lo JC, et al. A PGC1-α-dependent myokine that drives brown-fat-like development of white fat and thermogenesis. Nature. 2012;11(481):463-8. 5. Ozcelik O, Algul S, Yilmaz B. Nesfatin-1 and irisin levels in response to the soccer matches performed in morning, afternoon and at night in young trained male subjects. Cellular and molecular biology (Noisy-le-Grand, France). 2018;64:(10):130-33. 6. Zhang Y, Mu Q, Zhou Z, Song H, Wu F, Jiang M, et al. Protective effect of irisin on atherosclerosis via suppressing oxidized low density lipoprotein induced vascular inflammation and endothelial dysfunction. PLoS One. 2016; 11(6):e0158038. 7. Efe TH, Acar B, Ertem AG, Yayla KG , Algul E, Yayla C, et al. Serum irisin level can predict the severity of coronary artery disease in patients with stable angina. Korean Circulation Journal. 2017;47:44-9. 8. Mahgoub MO, D'Souza C, Al Darmaki RSMH, Baniyas MMYH, Adeghate E. An update on the role of irisin in the regulation of endocrine and metabolic functions. Peptides. 2018;104:15-23. 9. Deftereos S, Bouras G, Tsounis D, Papadimitriou C, Hatzis G, Raisakis K, et al. Association of asymmetric dimethylarginine levels with treadmill-stress-test-derived prognosticators. Clinical Biochemistry. 2014; 47(7-8):593-8 10. Alessio HM, Hagerman AE, Fulkerson BK, Ambrose J, Rice RE, Wiley RL. Generation of reactive oxygen species after exhaustive aerobic and isometric exercise. Medicine & Science in Sports & Exercise. 2000;32:1576–81.
Abstract
References
- 1. Wasserman K, Hansen JE, Sue DY, Stringer W, Sietsema KE, Sun XG, et al. Principles of Exercise Testing and Interpretation: Including Pathophysiology and Clinical Applications, Lippincott Williams & Wilkins, Philadelphia, PA, USA, 5th edition, 2012. 2. Ozcelik O, Ozkan Y, Algul S, Colak R. Beneficial effects of training at the anaerobic threshold in addition to pharmacotherapy on weight loss, body composition, and exercise performance in women with obesity. Patient Preference and Adherence. 2015;9:999-1004. 3. Ward SA, Whipp BJ. Kinetics of the ventilatory and metabolic responses to moderate-intensity exercise in humans following prior exercise-induced metabolic acidaemia. Advences in Experimental Medicine and Biology. 2010;669:323-6. 4. Boström P, Wu J, Jedrychowski MP, Korde A, Ye L, Lo JC, et al. A PGC1-α-dependent myokine that drives brown-fat-like development of white fat and thermogenesis. Nature. 2012;11(481):463-8. 5. Ozcelik O, Algul S, Yilmaz B. Nesfatin-1 and irisin levels in response to the soccer matches performed in morning, afternoon and at night in young trained male subjects. Cellular and molecular biology (Noisy-le-Grand, France). 2018;64:(10):130-33. 6. Zhang Y, Mu Q, Zhou Z, Song H, Wu F, Jiang M, et al. Protective effect of irisin on atherosclerosis via suppressing oxidized low density lipoprotein induced vascular inflammation and endothelial dysfunction. PLoS One. 2016; 11(6):e0158038. 7. Efe TH, Acar B, Ertem AG, Yayla KG , Algul E, Yayla C, et al. Serum irisin level can predict the severity of coronary artery disease in patients with stable angina. Korean Circulation Journal. 2017;47:44-9. 8. Mahgoub MO, D'Souza C, Al Darmaki RSMH, Baniyas MMYH, Adeghate E. An update on the role of irisin in the regulation of endocrine and metabolic functions. Peptides. 2018;104:15-23. 9. Deftereos S, Bouras G, Tsounis D, Papadimitriou C, Hatzis G, Raisakis K, et al. Association of asymmetric dimethylarginine levels with treadmill-stress-test-derived prognosticators. Clinical Biochemistry. 2014; 47(7-8):593-8 10. Alessio HM, Hagerman AE, Fulkerson BK, Ambrose J, Rice RE, Wiley RL. Generation of reactive oxygen species after exhaustive aerobic and isometric exercise. Medicine & Science in Sports & Exercise. 2000;32:1576–81.
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Health Care Administration |
| Journal Section | Original Research |
| Authors | |
| Publication Date | March 25, 2020 |
| Published in Issue | Year 2020 Volume: 10 Issue: 1 |