ETİLEN GLİKOLÜN MİDE ÜZERİNE ETKİSİ: HİSTOLOJİK ÇALIŞMA Effect of Ethylene Glycol on The Stomach: Histological Study
Abstract
ÖZET
Amaç: Etilen glikol (antifriz) yanlışlıkla ya da intihar amaçlı sindirim yoluyla alınması sonucu kısa sürede
şiddetli belirtiler göstermekte, yaşam kalitesini etkilemekte uygun tedavi edilmediği takdirde ölüme neden
olabilmektedir. Bu çalışmada etilen glikolün sıçan midesi üzerinde etkilerinin histolojik ve immunohistokimyasal
yöntemlerle araştırılması amaçlanmıştır.
Gereç ve Yöntem: Projede sıçanlar rastgele 2 gruba ayrılarak metabolik kafeslere yerleştirildi: Kontrol grubu
(6 adet) ve Etilen glikol grubu (grup EG) (6 adet). Grup EG sıçanlarının içme sularına 21 gün boyunca %1
etilen glikol eklendi. 21 günün sonunda sıçanlar ketamin + xylazin anestezisi altında uyutularak mideleri
alındı. Mide doku kesitlerine hematoksilen-eozin boyaması ve ghrelin-pozitif hücreleri belirlemek amacıyla
immünohistokimya uygulandı.
Bulgular: Kontrol grubuyla kıyaslandığında EG grubuna ait mide doku epitelizasyonunda bozulmalar gözlendi.
Ghrelin-immunopozitif hücre sayısında ise kontrol grubuna kıyasla EG grubunda artış olduğu belirlendi.
Sonuç: Sonuç olarak, ghrelin-immunopozitif hücre sayısındaki artışa bağlı olarak, ghrelin EG alımı sonrasında
bozulmuş fizyolojinin düzeltilmesine katkı sağlayabilir.
Anahtar Kelimeler: Etilen glikol; Mide; Sıçan; Ghrelin.
ABSTRACT
Aim: Ethylene glycol (antifreeze) may show severe symptoms in a short time as a result of accidental or
suicidal ingestion and may cause death if not treated appropriately. The aim of this study was to investigate
the effects of ethylene glycol on rat stomach by histological and immunohistochemical methods.
Materials and Methods: The rats were divided into two groups random, and placed in metabolic cages.
Control group (6 units) and Ethylene glycol group (group EG) (6 units). 1% ethylene glycol was added to the
drinking water of Group EG rats for 21 days. At the end of 21 days, rats were anesthetized under ketamine
+ xylazine anesthesia and their stomachs were removed. Hematoxylin-eosin staining and ghrelin-positive
cells were applied to the gastric tissue sections for immunohistochemistry.
Results: Deterioration of gastric tissue epithelialization of was observed EG group compared to control
group. There was an increase in the number of ghrelin-immunopositive cells in the EG group compared to
the control group.
Conclusion: As a result, due to the increase in the number of ghrelin-immunopositive cells, ghrelin may
contribute to the correction of impaired physiology after EG uptake.
References
- 1. Vale JA, Bluett NH, Widdop B. Ethylene glycol poisoning. Postgraduate medical journal. 1976;52(611):598-602. 2. Tuero G, Gonzalez J, Sahuquillo L, Freixa A, Gomila I, Elorza MA, et al. Value of glycolic acid analysis in ethylene glycol poisoning: A clinical case report and systematic review of the literature. Forensic science international. 2018;290:e9-e14. 3. Aşirdizer M, Gürpinar T, Yavuz Ms. Toksikolojik, klinik ve adli tıp yönleriyle etilen glikol zehirlenmesi. Turkiye Klinikleri Journal of Forensic Medicine and Forensic Sciences. 2010;7(2):79-90. 4. Van hee P, Neels H, De Doncker M, Maudens KE, Lambert W, Patteet L. Ethylene glycol poisoning: quintessential clinical toxicology; analytical conundrum. Clinica chimica acta; international journal of clinical chemistry. 2013;415:107-8. 5. Demirel İ, Levent A, Atalan G, Urfalioğlu A, Toprak GÇ. Antifriz İçen Bir Hastada Etilenglikol Zehirlenmesi: Olgu Sunumu. Fırat Tıp Dergisi. 2012;17(3):182-4. 6. Davis DP, Bramwell KJ, Hamilton RS, Williams SR. Ethylene glycol poisoning: case report of a record-high level and a review. The Journal of emergency medicine. 1997;15(5):653-67. 7. Kojima M, Hosoda H, Date Y, Nakazato M, Matsuo H, Kangawa K. Ghrelin is a growth-hormone-releasing acylated peptide from stomach. Nature. 1999;402(6762):656-60. 8. Kojima M, Hosoda H, Matsuo H, Kangawa K. Ghrelin: discovery of the natural endogenous ligand for the growth hormone secretagogue receptor. Trends in endocrinology and metabolism: TEM. 2001;12(3):118-22. 9. Sonmez MF, Karabulut D, Gunduz Y, Dundar M. The Effects of Long-Term Diabetes on Ghrelin Expression in Rat Stomachs. Advances in clinical and experimental medicine : official organ Wroclaw Medical University. 2015;24(3):401-7. 10. Yakan B, Arzu Y, Rihtim T, Doğanyiğit Z. Dişi Siçan Midesinde Ghrelin Ekspresyonu Üzerine Ovariektominin Etkilerinin Analizi. Bozok Tıp Dergisi.7(1):22-31.
Abstract
References
- 1. Vale JA, Bluett NH, Widdop B. Ethylene glycol poisoning. Postgraduate medical journal. 1976;52(611):598-602. 2. Tuero G, Gonzalez J, Sahuquillo L, Freixa A, Gomila I, Elorza MA, et al. Value of glycolic acid analysis in ethylene glycol poisoning: A clinical case report and systematic review of the literature. Forensic science international. 2018;290:e9-e14. 3. Aşirdizer M, Gürpinar T, Yavuz Ms. Toksikolojik, klinik ve adli tıp yönleriyle etilen glikol zehirlenmesi. Turkiye Klinikleri Journal of Forensic Medicine and Forensic Sciences. 2010;7(2):79-90. 4. Van hee P, Neels H, De Doncker M, Maudens KE, Lambert W, Patteet L. Ethylene glycol poisoning: quintessential clinical toxicology; analytical conundrum. Clinica chimica acta; international journal of clinical chemistry. 2013;415:107-8. 5. Demirel İ, Levent A, Atalan G, Urfalioğlu A, Toprak GÇ. Antifriz İçen Bir Hastada Etilenglikol Zehirlenmesi: Olgu Sunumu. Fırat Tıp Dergisi. 2012;17(3):182-4. 6. Davis DP, Bramwell KJ, Hamilton RS, Williams SR. Ethylene glycol poisoning: case report of a record-high level and a review. The Journal of emergency medicine. 1997;15(5):653-67. 7. Kojima M, Hosoda H, Date Y, Nakazato M, Matsuo H, Kangawa K. Ghrelin is a growth-hormone-releasing acylated peptide from stomach. Nature. 1999;402(6762):656-60. 8. Kojima M, Hosoda H, Matsuo H, Kangawa K. Ghrelin: discovery of the natural endogenous ligand for the growth hormone secretagogue receptor. Trends in endocrinology and metabolism: TEM. 2001;12(3):118-22. 9. Sonmez MF, Karabulut D, Gunduz Y, Dundar M. The Effects of Long-Term Diabetes on Ghrelin Expression in Rat Stomachs. Advances in clinical and experimental medicine : official organ Wroclaw Medical University. 2015;24(3):401-7. 10. Yakan B, Arzu Y, Rihtim T, Doğanyiğit Z. Dişi Siçan Midesinde Ghrelin Ekspresyonu Üzerine Ovariektominin Etkilerinin Analizi. Bozok Tıp Dergisi.7(1):22-31.
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Health Care Administration |
| Journal Section | Original Research |
| Authors | |
| Publication Date | June 8, 2020 |
| Published in Issue | Year 2020 Volume: 10 Issue: 2 |