Biofilm Forming Capacity and Presence of Biofilm-Associated Virulence Genes of Enterococcus faecalis isolates from Slaughterhouse Environments

Year 2023, , 12 - 17, 30.06.2023

Candan Güngör , Dursun Alp Gündoğ , Nurhan Ertaş Onmaz

https://doi.org/10.58833/bozokvetsci.1283247

Cited By: 3

Abstract

This study aims to investigate the presence of biofilm-producing enterococcus and their biofilm-related virulence factor genes in the slaughter line of a class A cattle slaughterhouse in Kayseri. A total of 300 samples (180 carcasses, 102 equipment and surfaces, and 18 samples of slaughterhouse wastewater) were analyzed using conventional methods and PCR. The biofilm-forming abilities of the isolates were determined using congo red agar and microplate testing, and PCR was used to detect biofilm-associated virulence genes (gelE and esp). E. faecalis was isolated from 40 (13.3%) of analyzed samples, of which 35 (87.5%) produced biofilms. The gelE gene was detected in 33 (82.5%) biofilm-positive isolates, from which one (2.5%) contained also esp gene. In conclusion, this study determined the presence of biofilm-positive E. faecalis among the samples taken from a slaughterhouse in Kayseri province, and the relationship between virulence genes and biofilm formation. In conclusion, it is important for food safety to detect biofilm-positive E. faecalis, reflecting the hygienic quality of the slaughterhouse, in all sample types taken from the slaughterhouse in Kayseri, determining the relationship between the biofilm formation of isolates and virulence factor genes.

Keywords

E. faecalis, carcass, cattle, slaughterhouse, slaughterhouse wastewater

Mezbaha Ortamından İzole edilen Enterococcus faecalis izolatlarının Biyofilm Oluşturma Kapasitesi ve Biyofilm ile İlişkili Virülans Genlerin Varlığı

Abstract

Bu çalışmada, Kayseri ilinde mevcut olan birinci sınıf bir sığır mezbahasında kesim hattı boyunca alınan örneklerde biyofilm oluşturma yeteneğine sahip E. faecalis varlığı ve izolatlar da biyofilm ile ilişkili virülans faktör genlerinin araştırılması amaçlandı. Çalışmada, kesimhaneden alınan toplam 300 adet örnekte (180 karkas, 102 ekipman ve yüzey ve 18 mezbaha atık suyu) etkenin varlığı konvansiyonel yöntem ve PZR ile analiz edildi. İzolatların biyofilm oluşturma yeteneklerini belirlemede Kongo kırmızısı agar ve mikroplaka testi ve biyofilm ile ilişkili virülans genlerinin (gelE ve esp) tespitinde PZR kullanıldı. Analiz edilen 300 örneğin 40’ından (%13.3) E. faecalis izole edildi ve bu izolatların 35 (%87.5) biyofilm oluşturma yeteneğinde idi. Biyofilm pozitif olan izolatların 33’ünde (%82.5) gelE geni belirlendi, ayrıca bu izolatların bir tanesini (% 2.5) esp genini de içeriyordu. Sonuç olarak, Kayseri ilindeki kesimhaneden alınan tüm örnek türlerinde mezbahanın hijyenik kalitesini yansıtan biyofilm oluşturma yeteneğine sahip E. faecalis’in tespit edilmesi ve izolatların biyofilm oluşumunun virülans faktör genler ile ilişkisi tespit edilmesi gıda güvenliği açısından önemlidir.

Keywords

karkas, mezbaha, mezbaha atık suyu, sığır, E. faecalis

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