Investigation of Biofilm Forming Abilities of Slaughterhouse and Dairy Originated Pathogenic Bacteria by Congo Red Agar and Microplate Methods

Year 2023, , 18 - 26, 30.06.2023

Dursun Alp Gündoğ , Güven Güngör , Candan Güngör , Nurhan Ertaş Onmaz , Zafer Gönülalan

https://doi.org/10.58833/bozokvetsci.1287983

Cited By: 1

Abstract

In this study, a comparison of the efficacy and sensitivity of Congo Red Agar (CCA) and Microplate (MP) methods in determining the biofilm-forming abilities of Staphylococcus aureus, Listeria spp, Escherichia coli, and Salmonella spp isolates isolated from slaughterhouses and dairy farms and posing a risk to public health was intended. It was found that 51.1% of 135 isolates were identified as biofilm producers in CCA and 53% in MP. Among the isolates analysed, the CCA method's sensitivity was highest in S. aureus (97%), followed by Listeria spp. (59%), Salmonella spp (17%). and E. coli (13%). The specificity rates were 89%, 59% and 39% for E. coli, Salmonella spp. and Listeria spp., respectively. However, since all S. aureus isolates gave positive biofilm results in the MP test, the specificity of CCA could not be determined for this isolate. As a result, the sensitivity of the CCA method was higher only for S. aureus among the analysed isolates, while the selectivity of this method was good for other isolates. It is important to accurately determine the biofilm abilities of pathogen strains that may pose a risk to public health in food and food processing environments. Therefore, it was concluded that the complementary application of CCA with the MP method is important for the reliability of the results and the detection of biofilms.

Keywords

Biofilm, Congo Red Agar, Food pathogens, Microplate

Çeşitli Gıda Kaynaklı Patojenik Bakterilerin Biyofilm Oluşturma Yeteneklerinin Tespitinde Kongo Kırmızısı Agar’ın Etkinliğinin Değerlendirilmesi

Abstract

Bu çalışmada, daha önceki çalışmalarla mezbaha ve mandıralardan izole edilen ve halk sağlığı açısından risk oluşturan önemli patojenlerden Staphylococcus aureus, Listeria spp., Escherichia coli ve Salmonella spp. izolatlarının biyofilm oluşturma yeteneklerinin belirlenmesinde Kongo Kırmızısı Agar (KKA) ve Mikroplak (MP) yöntemlerinin etkinliğinin ve duyarlılıklarının karşılaştırılması amaçlandı. Çalışma sonucunda 135 izolatın %51.1’i KKA’da, %53’ü ise MP’de biyofilm üreticisi olarak belirlendi. Analiz edilen izolatlar arasında KKA yönteminin duyarlılığının en yüksek olduğu izolat S. aureus idi (%97) bunu Listeria spp. (%59), Salmonella spp (%17). ve E. coli (%13) izledi. Listeria spp., Salmonella spp .ve E. coli seçicilik oranları ise sırasıyla %39, %59 ve %89 idi. Ancak S. aureus izolatlarının tamamı MP testinde biyofilm pozitif sonuç verdiği için KKA’nın seçiciliği bu izolat için belirlenemedi. Sonuç olarak, KKA yönteminin duyarlılığı analiz edilen izolatlardan sadece S. aureus için yüksekti, diğer izolatlarda ise bu yöntemin seçiciliğinin iyi olduğu görüldü. Gıda ve gıda işleme ortamlarında halk sağlığı için risk oluşturabilen patojen suşlarına ait biyofilm yeteneklerinin doğru tespit edilmesi önem arz etmektedir. Bu nedenle, KKA’nın MP yöntemi ile tamamlayıcı bir şekilde uygulanması sonuçların güvenirliği ve biyofilmlerin tespiti için önemli olduğu sonucuna varıldı.

Keywords

Biyofilm, Gıda patojenleri, Kongo Kırmızısı Agar, Mikroplak

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