The investigation of the effect of two different heavy metals (copper and zinc) on the medicinal leeches (Hirudo verbana)

Year 2024, Volume: 5 Issue: 2, 70 - 72

Erdal Yılmaz , Ebubekir Atici

https://doi.org/10.58833/bozokvetsci.1600024

Abstract

In the study, the effects of different concentrations of zinc and copper metals on medicinal leeches were investigated. Fifteen medicinal leeches (Hirudo verbana), which were stocked in containers with 10 liters of water, were exposed to two different doses of copper and zinc metals, which were determined below the lethal doses. While applied doses of zinc and copper were 3.0 mg/L, 12 mg/L, and 0.15 mg/L and 0,6 mg/L during 96 hours, respectively. Applied doses of zinc and copper were 0,5 mg/L and 2,0 mg/L, and 0,02 mg/L and 0,08 mg/L for 30 days applications, respectively. After these applications, antimicrobial characteristics of the leech saliva, heavy metal deposition and malondialdehyde (MDA) levels were analysed. It was determined that leech saliva extract did not show any antimicrobial activity on fish pathogens, Lactococcus garviae and Yersinia ruckeri. Also, It has been found that when medicinal leeches exposed to zinc and copper metals, heavy metals concentrations in tissues of the medicinal leeches increased. In addition, it was found that malondialdehyde values in leech saliva increased when zinc and copper were exposed at different concentrations and durations. It was revealed from the data that medicinal leech of the Hirudo verbana species is a good biomonitor organism for zinc and copper heavy metals.

Keywords

Antimicrobial, heavy metal, Hirudo verbana, medicinal leech, malondialdehyde

İKİ FARKLI AĞIR METALİN (BAKIR VE ÇİNKO) TIBBİ SÜLÜKLER (HİRUDO VERBANA) ÜZERİNE ETKİLERİNİN ARAŞTIRILMASI

Abstract

Araştırmada çinko ve bakır metallerinin farklı konsantrasyonlarının tıbbi sülükler üzerindeki etkileri incelenmiştir. 10 litrelik kaplara 15 adet olacak şekilde stoklanan tıbbi sülükler (Hirudo verbana) bakır ve çinko metallerinin letal dozların altında belirlenen farklı iki doza maruz bırakılmıştır. Çinko ve bakır için 96 saatlik muamele dozları sırası ile 3.0 mg/L ile 12 mg/L ve 0.15 mg/L ile 0.6 mg/L iken; 30 günlük muamelelerde 0.5 mg/L ile 2.0 mg/L ve 0.02 mg/L ile 0.08 mg/L olarak uygulanmıştır. Bu uygulamalardan sonra sülüklerden alınan salya örneklerinin antimikrobiyal karakteristiği, ağır metal birikimleri ve malondialdehyde (MDA) düzeyleri analiz edildi. Çalışmada balık patojenlerinden Lactococcus garviae ve Yersinia ruckeri’ ye karşı sülük salyasının herhangi bir antimikrobiyal etki göstermediği belirlenmiştir. Tıbbi sülüklerin çinko ve bakır metallerine maruz kaldıklarında dokularında depolanan ağır metal miktarlarının arttığı tespit edilmiştir. Ayrıca çinko ve bakırın farklı konsantrasyon ve sürelerde maruziyetinde sülük salyasında malondialdehit değerlerinin de artış gösterdiği bulunmuştur. Elde edilen verilerden Hirudo verbana türü tıbbi sülüğün çinko ve bakır ağır metalleri için iyi bir biyo-monitör organizma olduğu ortaya konulmuştur.

Keywords

Ağır metal, antimikrobiyal, Hirudo verbana, malondialdehit, tıbbi sülük

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