Effect of Streptomyces sp VYN22 Strain on Dye Removal in Textile Wastewater

Abstract

Dyes and dyestuffs used in the textile industry cause environmental pollution by showing toxic, carcinogenic and mutagenic effects when they are released into water without any treatment. Environmentally friendly methods based on bioremediation for the disposal of azo dyes, which are especially in the pigment dyes group and resistant to microbial degradation, from textile wastewater are of interest. Actinobacteria are bacteria involved in bioremediation and biodegradation processes in nature and play a key role in the cycling of organic matter and carbon. In this study, it was aimed to identify an Actinobacterium isolate from soil by 16S rRNA sequence analysis and to eliminate azo dyes from textile wastes by dye removal using the bacterium identified as Streptomyces sp VYN22. According to 16S rRNA sequence analysis, this isolate was 99.71% closely related to Streptomyces bobili type strain. Using Colorsol Orange Deep textile dye at different pHs, the dyestuff removal of live, dry and lyophilized forms of Streptomyces sp VYN22 at 0-10 hours was investigated by spectrophotometric measurements. At the end of the study, it was observed that these bacteria gave high results in dyestuff removal at pH 4, 6 and 10.

Keywords

• Actinobacteria
• Azo dye
• Biodegradation

Streptomyces sp. VYN22 Suşunun Tekstil Atık Sularında Boyar Madde Giderimine Etkisi

Abstract

Tekstil endüstrisinde kullanılan boyalar ve boyar maddeler herhangi bir işlem görmeden sulara bırakıldıklarında toksik, kanserojen ve mutajenik etki göstererek çevre kirliliğine neden olmaktadır. Özellikle pigment boyar maddeler grubunda yer alan ve mikrobiyal bozunmaya karşı dirençli olan azo boyaların, tekstil kaynaklı atık sulardan bertarafı için biyoremediasyona dayalı çevre dostu yöntemler ilgi çekmektedir. Aktinobakteriler, doğada biyoremediasyon ve biyodegredasyon süreçlerine dahil olan ve organik madde ile karbon döngüsünde kilit rol oynayan bakterilerdir. Bu çalışmada, topraktan izole edilen Aktinobakteri izolatının 16S rRNA dizi analizleri ile tanımlanması ve Streptomyces sp. VYN22 olarak belirlenen bakterinin kullanılarak tekstil atıklarından azo boyaların boyar madde giderimi ile ortadan kaldırılması amaçlanmıştır. Bu izolatın 16S rRNA dizi analizlerine göre Streptomyces bobili tip türü ile %99,71 yakın akraba olduğu belirlenmiştir. Farklı pH’larda Colorsol Orange Deep tekstil boyası kullanılarak, Streptomyces sp. VYN22’ nin canlı, kuru ve liyofilize formlarının 0-10 saatlerdeki boyar madde giderimleri spektrofotometrik ölçümlerle incelenmiştir. Çalışma sonunda bu bakterilerin pH 4, 6 ve 10’da boyar madde gideriminde yüksek sonuçlar verdiği gözlemlenmiştir.

Keywords

• Aktinobakteri
• Azo boya
• Biyodegredasyon

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