Determination of the Chemical Content of Shilajit in Terms of Ten Different Polyphenolic Compounds by UAE Method and HPLC Analysis

Abstract

Shilajit (Mumio) is a complex of natural bioactive compounds that has historically been used as a therapeutic cream for many skin diseases. It is also used in traditional medicine for diseases such as diabetes Alzheimer's and cancer. In this study, to determine the polyphenolic content of Shilajit, ultrasound assisted extracts (UAE) were obtained at 45 °C and analyzed by High Performance Liquid Chromatography-Ultraviolet (HPLC-UV) for the quantitative analysis of ten different polyphenolic compounds. Such a comprehensive content analysis has not been done for Shilajit before. As a result of the experimental study, 28.99±1.23 µg/g rutin, 25.47±1.67 µg/g ferulic acid, 41.49±0.41 µg/g resveratrol and 532.19±8.21 µg/g taxifolin was determined. In this study, 532.19±8.21 µg/g of taxifolin, which is found to be very effective against many diseases in the literature, is a very high value compared to the literature. The results of this research will shed an important light to researchers working in the fields of extraction of bioactive substances, food supplement production and pharmaceutical applications.

Keywords

• Shilajit
• Mumio
• Polyphenols
• Taxifolin
• Resveratrol
• HPLC

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