Glikoz/hava enzimatik yakıt hücresi kullanılarak tek çip üzerinde glikoz tayini

Abstract

Diyabet hastalığı, kronik ve metabolik bir rahatsızlık olup günümüzde birçok insanın yaşam kalitesini etkilemekte ve giderek büyüyen bir sorun haline gelmektedir. Bu nedenle kandaki glikoz miktarının takip edilmesi ve belirlenmesi diyabet tanısı için büyük öneme sahiptir. Bu çalışmada, glikoz/hava enzimatik yakıt hücresi kullanılarak kendi kendine çalışabilen bir glikoz tayin çipi geliştirilmiştir. Enzimatik yakıt hücresinin anodu ferosen-nafyon içeren çok duvarlı karbon nanotüpler ile modifiyeli elektrotlara glikoz oksidaz enziminin tutuklanmasıyla; katodu ise, asitle muamele edilmiş tek duvarlı karbon nanotüple modifiyeli elektrotlara bilirubin oksidaz enziminin tutuklanmasıyla hazırlanmıştır. Hazırlanan anot ve katot sırasıyla glikoz ve oksijen substratları kullanılarak elektrokimyasal olarak karakterize edilmiştir.  Tek çip üzerinde hazırlanan enzimatik yakıt hücresi 0-3 mM glikoz konsantrasyonu aralığında lineer sonuç vererek 32 mV/mM hassasiyetinde glikoz tayini sağlamış ve 30 saniye gibi kısa bir sürede sonuç vermiştir.

Keywords

• biyosensör,glikoz oksidaz,bilirubin oksidaz,enzimatik yakıt hücresi,diyabet

A self-powered detection of glucose using glucose/air enzymatic fuel cell on a single chip

Abstract

Diabetes is a chronic and metabolic disorder that affects many people's quality of life and is becoming a growing problem. Therefore, monitoring and determining the amount of glucose in the blood is of great importance for the diagnosis of diabetes. In this study, a self-powered glucose determination was developed using glucose/air enzymatic fuel cell (EnFC) on a single chip. The anode of the EnFC is prepared by immobilizing glucose oxidase enzyme on ferrocene-nafion containing multi-walled carbon nanotube modified electrodes; the cathode, on the other hand, was prepared by immobilizing bilirubin oxidase enzyme on acid-treated single-walled carbon nanotube modified electrodes. The prepared anode and cathode were electrochemically characterized using glucose and oxygen substrates respectively. EnFC prepared on a single chip showed a linear response in the range of 0-3 mM glucose concentrations, achieving 32 mV/mM sensitivity and a response time of 30 seconds.

Keywords

• biosensor,glucose oxidase,bilirubin oxidase,enzymatic fuel cell,diabetes

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