Vatoz (Dasyatis pastinaca) Balığı Kas Dokusunun Enzimatik Hidrolizi ve Hidrolizatların Antioksidan ve Bakır Şelatlama Aktivitelerinin Değerlendirilmesi

Year 2020, , 680 - 687, 30.12.2020

Ebru Kocadağ Kocazorbaz , Meliha Kanatsızoğlu , Hanife Ttuğçe Çeker , Esra Menfaatli

https://doi.org/10.35193/bseufbd.665435

Abstract

Biyoaktif peptidler canlıların vücut fonksiyonlarına ve sağlığına olumlu etkileri bulunan spesifik protein fragmentleri olup bitkisel, hayvansal, fungal, bakteriyel ve venom kaynaklı olabilmektedir. Farklı protein kaynaklarından keşfedilen biyoaktif peptidlerin çoğu, antihipertansiyon, antioksidan, antitrombotik, antimikrobiyal ve antikanser immünomodülasyon gibi çeşitli biyolojik özellikler göstermiştir. Bu çalışmanın amacı, vatoz (Dasyatis pastinaca) kas dokusu protein hidrolizatının biyolojik aktivitelerinin değerlendirilmesidir. Bu nedenle, vatozun homojenize kas dokusu, esperaz ve tripsin enzimleri tarafından hidrolize edildi. Enzim/substrat oranı, hidroliz süresi ve derecesi optimize edilerek hidrolizatların antioksidan (CUPRAC yöntemi) ve metal şelatlama (bakır şelatlama) aktiviteleri değerlendirildi.

Keywords

biyoaktif peptid, enzimatik hidroliz, antioksidan, esperaz, tripsin

Enzymatic Hydrolysis of Stingray (Dasyatis pastinaca) Muscle and Evaluation of Antioxidant and Copper Chelating Activities of the Hydrolysates

Abstract

Bioactive peptides that originate from different sources, such as plant, animal, fungi, bacteria, and venom, are specific protein fragments that have positive effects on the body functions and health of living organisms. Most of the discovered bioactive peptides from different protein sources showed various biological features, such as antihypertension, antioxidant, antithrombotic, antimicrobial, and anticancer immunomodulation. The aim of this study is the evaluation of biological activities of stingray (Dasyatis pastinaca) muscle tissue protein hydrolysate. Therefore, the homogenized muscle tissue of stingray was hydrolyzed by esperase and trypsin enzymes. Enzyme/substrate ratio, hydrolysis time, and degree were optimized. Moreover, the antioxidant (CUPRAC method) and metal chelating (copper chelating) activities of the hydrolysates were evaluated.

Keywords

bioactive peptide, enzymatic hydrolysis, antioxidant, esperase, trypsin

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