2-Tiyourasilin Tayini için Poli(Bromokrezol moru) ile Modifiye Camsı Karbon Elektroda Dayalı Elektrokimyasal DNA Sensörü Geliştirilmesi

Year 2020, Volume: 7 Issue: 100. Yıl Özel Sayı, 229 - 242, 23.03.2020

Derya Koyuncu Zeybek , Burcu Demir Doğancı Müzeyyen Özge Karaşallı

https://doi.org/10.35193/bseufbd.642171

Abstract

Bu çalışmada, potansiyel bir kanser ilacı olan 2-tiyourasil (2-TU)
ilacı ile balık sperminden elde edilen çift zincirli deoksiribonükleik asit
(dsDNA) molekülü arasındaki etkileşim incelenmiş ve bu etkileşime dayalı olarak
ilacın elektrokimyasal tayini gerçekleştirilmiştir. Bunun için, camsı karbon
elektrot (GCE) yüzeyi, bromokrezol moru (BCP) monomerinin elektrokimyasal
polimerizasyonu ile modifiye edilmiş ve bu elektrot (GCE/P(BCP)) yüzeyine,
dsDNA elektrokimyasal olarak immobilize edilmiştir (GCE/P(BCP)/dsDNA). dsDNA
ile 2-TU arasındaki etkileşim mekanizması diferansiyel puls voltametri
yöntemiyle araştırılmıştır. Bu etkileşim sonrası guaninin yükseltgenme pik
akımında azalma gözlenmiş ve bu azalmaya bağlı olarak 2-TU’in elektrokimyasal
tayini indirekt yöntemle gerçekleştirilmiştir. 2-TU için doğrusal çalışma aralığı
0,1-50 mg L⁻¹ ve gözlenebilme sınırı 0,033 mg L⁻¹ olarak
bulunmuştur. 2-TU−dsDNA etkileşim mekanizması UV-Görünür bölge moleküler
absorpsiyon spektroskopi yöntemiyle de incelenmiştir. Hazırlanan DNA
biyosensörüne bozucu etki yapabilecek türlerin etkisi araştırılmış ve ayrıca
2-TU ilacının idrar numunesinde tayini gerçekleştirilmiştir. Deneysel
çalışmalardan elde edilen sonuçlara göre, 2-TU ve dsDNA arasındaki başlıca
etkileşim modunun interkalasyon olduğu belirlenmiştir.

Keywords

2-Tiyourasil, DNA Sensörü, Poli(bromokrezol moru), DNA-ilaç etkileşimi

Supporting Institution

Kütahya Dumlupınar Üniversitesi Bilimsel Araştırma Projeleri Birimi

Project Number

2015-41

Thanks

Yazarlar 2015-41 nolu proje ile destek sağlayan Kütahya Dumlupınar Üniversitesi Bilimsel Araştırma Projeleri Birimine ve Burcu Demir’e yüksek lisans tez dönemi boyunca Yurtiçi Öncelikli Alanlar Yüksek Lisans Burs Programı (2210-C) kapsamında burs sağlayan Türkiye Bilimsel ve Teknolojik Araştırma Kurumuna (TÜBİTAK) teşekkür ederler.

Development of Electrochemical DNA Sensor Based on Poly (Bromocresol purple) Modified Glassy Carbon Electrode for the Determination of 2-Thiouracil

Abstract

In this study, the interaction
between 2-thiouracil (2-TU), a potential cancer drug, and double chain
deoxyribonucleic acid (dsDNA) molecule obtained from fish sperm was investigated
and electrochemical determination of the drug was performed. For this, the
glassy carbon electrode (GCE) surface was modified by electrochemical
polymerization of the bromocresol purple (BCP) monomer and dsDNA was
electrochemically immobilized (GCE/P(BCP)/dsDNA) onto the surface of this
electrode (GCE/P(BCP)).  The interaction
mechanism between dsDNA and 2-TU was investigated by differential pulse
voltammetry method. After this interaction, a decrease in the oxidation peak
current of guanine was observed and electrochemical determination of 2-TU was
performed by indirect method due to this decrease. The linear operating range
for 2-TU was 0.1-50 mg L^{− 1} and the detection limit was 0.033 mg L⁻¹.
The interaction mechanism of 2-TU − dsDNA was also investigated by UV-Visible
molecular absorption spectroscopy. The effect of the species that may cause
disruptive effect on the DNA biosensor was investigated and 2-TU drug was
determined in the urine sample. According to the results obtained from
experimental studies, the main mode of interaction between 2-TU and dsDNA is
intercalation.

Keywords

2-Thiouracil, DNA Sensor, Poly(Bromocresol Purple), DNA-Drug Interaction

Project Number

2015-41

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