Bitki Proteomik Çalışmalarında Kullanılan Yaklaşımlar ve Uygulama Yöntemleri

Year 2020, Volume: 7 Issue: 1, 497 - 528, 28.06.2020

Aslihan Gunel , Semra Hasancebi , Talat Yalçın , Mahmut Emir , Yahya Emin Demirci , Melike Dinç , Melda Güray

https://doi.org/10.35193/bseufbd.667838

Abstract

Proteomik yaklaşımları 2000 li yılların başlarına kadar mikroorganizmalar ve hayvansal kaynaklı örneklerde ağırlıklı olarak kullanıldı. Bu dönemde bitki proteomik çalışmaları yok denecek kadar azdır. Bitkisel dokulardaki sert hücre çeperleri, karmaşık ve çok çeşitli sekonder metabolitlerin varlığı, fazla miktardaki pigmentler, proteazlar, polifenoller, polisakkaritler, nişasta ve lipitler total protein örneklerinin hazırlanması ve proteinlerin ayrımı sırasında pek çok soruna neden olmuştur. Ancak her bir sorunun üstesinden gelmek üzere sürdürülen çabalar sayesinde bitki dünyasında da proteomik yaklaşım kullanımı yaygınlaşmıştır. Bu derlemede, örnek hazırlığından protein tanımlamaya kadar tüm basamaklar yöntemsel gelişmeleri de kapsayacak şekilde ayrıntılı olarak ele alınmış ve konuyla ilgili araştırıcıların maksimum yararlanabileceği bir kaynak oluşturulmaya çalışılmıştır.

Keywords

Proteomiks, bitki proteomik, Kütle spektrometresi, 2D-PAGE, 2D-LC

Supporting Institution

Çalışma bir derleme çalışmasıdır.

Approachs and Application Methods in Plant Proteomics Research

Abstract

Proteomics approach was used mainly in the samples for microorganisms and animal tissues until early 2000s, the period during which the plant proteomics studies were scarcely available. Tough nature of cell membranes, existence of complex and diverse range of secondary metabolites, high abundance of pigments, proteases, polyphenols, polysaccharides and lipids caused a number of problems for preparation and resolution of the protein samples. However, proteomics approach in plant area had become widespread with the help of continuous efforts in order to overcome these difficulties. This review is prepared comprehensively from the preparation of the samples to the identification of the proteins and it aims to serve as a detailed source for the researchers interested in plant proteomics, especially for young investigators.

Keywords

Proteomics, plant proteomics, mass spectrometry, 2D-PAGE, 2D-LC

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