Year 2020, Volume 7 , Issue 2, Pages 544 - 552 2020-12-30

Cytotoxic Potential of Solanum lycopersicum Leaves Extract on Different Human Cell Lines
Solanum lycopersicum Yaprak Ekstraktının Farklı İnsan Hücre Hatlarındaki Sitotoksik Potansiyeli

Mehlika ALPER [1] , Hatice GÜNEŞ [2]


Plants have been traditionally used as a remedy against various diseases. In this study, the potential cytotoxic effects of the ethanol extract of leaves of Solanum lycopersicum were investigated on A549, HeLa, PC-3, MCF-7 and HEK293 cell lines. MTT assay was used for determination of cell viability. Apoptosis and activity of caspase-3 in A549 and HeLa cells treated with the extract were also investigated. At a concentration of 31.25 µg mL-1, this extract caused more than 50% cell death in the cancer cells that were tested for 72 h. It was determined that the extract of S. lycopersicum induced apoptosis in A549 and HeLa cells. Moreover, the extract of S. lycopersicum leaves caused significant caspase-3 activity in same cells. These findings may basically contribute to research related with the use of the extract of leaves of S. lycopersicum for development of anticancer agents.
Bitkiler geleneksel olarak çeşitli hastalıklara karşı çare olarak kullanılmaktadır. Bu çalışmada Solanum lycopersicum yapraklarının etanol ekstraktının potansiyel sitotoksik etkileri A549, HeLa, PC-3, MCF-7 ve HEK293 hücre hatlarında araştırılmıştır. Hücre canlılığının belirlenmesi için MTT deneyi kullanılmıştır. Ekstarakt ile muamele edilen A549 ve HeLa hücrelerinde apoptoz ve kaspaz-3 aktivitesi araştırılmıştır. 31.25 µg mL-1 konsantrasyonda, bu ekstrakt, 72 saatte test edilen kanser hücrelerinde % 50'den fazla sitotoksisiteye neden olmuştur. S. lycopersicum ekstraktının A549 ve HeLa hücrelerinde apoptozu indüklediği belirlenmiştir. Ayrıca, S. lycopersicum yapraklarının ekstresi aynı hücrelerde önemli kaspaz-3 aktivitesine neden olmuştur. Bu bulgular temel olarak antikanser ajanların gelişimi için S. lycopersicum yaprak ekstraktının kullanımı ile ilgili araştırmalara katkıda bulunabilir.
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Primary Language en
Subjects Basic Sciences
Journal Section Articles
Authors

Orcid: 0000-0001-6193-346X
Author: Mehlika ALPER
Institution: MUĞLA SITKI KOÇMAN ÜNİVERSİTESİ
Country: Turkey


Orcid: 0000-0001-5191-365X
Author: Hatice GÜNEŞ (Primary Author)
Institution: MUĞLA SITKI KOÇMAN ÜNİVERSİTESİ
Country: Turkey


Dates

Application Date : January 30, 2020
Acceptance Date : June 25, 2020
Publication Date : December 30, 2020

APA Alper, M , Güneş, H . (2020). Cytotoxic Potential of Solanum lycopersicum Leaves Extract on Different Human Cell Lines . Bilecik Şeyh Edebali Üniversitesi Fen Bilimleri Dergisi , 7 (2) , 544-552 . DOI: 10.35193/bseufbd.682245