Year 2021, Volume 8 , Issue 1, Pages 1 - 7 2021-06-30

Purification and Characterization of a Lectin From Bulbs of Cyclamen Mirabile
Cyclamen Mirabile Soğanından Lektin Saflaştırılması ve Karakterizasyonu

Ebru KOCADAĞ KOCAZORBAZ [1] , Ayşe Yağmur AZBAZDAR [2] , Merve ALİAKAR ÖZTÜRK [3] , Esra MENFAATLİ [4]


Lectin from the bulbs of Cyclamen mirabile which is an endemic species of Turkey was successfully isolated by affinity precipitation with alginate in one step. The purified protein produced two bands showing a dimeric structure in SDS-PAGE (13.5 and 14.8 kDa). C. mirabile lectin showed activity and stability in a broad pH scale and kept its haemagglutination activity in the temperature range of 4-40°C. MgCl2 and HgCl2 inhibited the haemagglutination activity of the lectin. In this study, a practical and efficient purification procedure was carried out for C. mirabile lectin by using affinity precipitation with alginate.

Bu çalışmada, Türkiye'nin endemik bir türü olan Cyclamen mirabile soğanından tek adımda lektin saflaştırıldı. Yöntem olarak, amonyum sülfat ve ardından kalsiyum aljinat presipitasyonu kullanıldı. Saflaştırılan lektin SDS PAGE ile analiz edildiğinde 13.5 ve 14.8 kDa molekül kütlelerine sahip iki protein bandı gözlendi. Hemaglütinasyon inhibisyon yöntemi ile C. mirabile soğanından elde edilen lektinin mannoz spesifik lektin olduğu belirlendi. C. mirabile lektini geniş bir pH aralığında ve 4-40°C arasında hemaglütinasyon aktivitesini korudu. MgCl2 ve HgCl2 metallerinin lektinin hemaglütinasyon aktivitesini inhibe ettiği görüldü.
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Primary Language en
Subjects Basic Sciences
Journal Section Articles
Authors

Orcid: 0000-0001-5611-5235
Author: Ebru KOCADAĞ KOCAZORBAZ (Primary Author)
Institution: EGE ÜNİVERSİTESİ
Country: Turkey


Orcid: 0000-0003-0806-1003
Author: Ayşe Yağmur AZBAZDAR
Institution: DOKUZ EYLÜL ÜNİVERSİTESİ
Country: Turkey


Orcid: 0000-0002-5897-4049
Author: Merve ALİAKAR ÖZTÜRK
Institution: EGE ÜNİVERSİTESİ
Country: Turkey


Orcid: 0000-0002-2370-6415
Author: Esra MENFAATLİ
Institution: EGE ÜNİVERSİTESİ
Country: Turkey


Dates

Application Date : February 6, 2020
Acceptance Date : August 23, 2020
Publication Date : June 30, 2021

APA Kocadağ Kocazorbaz, E , Azbazdar, A , Aliakar Öztürk, M , Menfaatli, E . (2021). Purification and Characterization of a Lectin From Bulbs of Cyclamen Mirabile . Bilecik Şeyh Edebali Üniversitesi Fen Bilimleri Dergisi , 8 (1) , 1-7 . DOI: 10.35193/bseufbd.685652