Bromofenol Mavisinin Pseudochloris wilhelmii ile Giderimi ve Daphnia magna Üzerindeki Akut Toksisitesi

Year 2025, Volume: 12 Issue: 2, 456 - 465, 30.11.2025

Elif Betül Kağızman , Şeyda Fikirdesici Ergen , Burcu Ertit Taştan

https://doi.org/10.35193/bseufbd.1663157

Abstract

Boyaların farklı endüstriyel alanlarda kullanımı artmakta ve bu durum çevre sorunlarını da beraberinde getirmektedir. Boyalar atıksulardan farklı birçok fiziksel ve kimyasal arıtma tekniği ile giderilse de çevre dostu giderim teknolojilerine ihtiyaç duyulmaktadır. Pseudochloris wilhelmii birçok biyoteknolojik çalışmada kullanılmasına rağmen bromofenol mavisi giderimi ve toksisitesi henüz araştırılmamıştır. Ayrıca bromofenol mavisi boyasının biyolojik giderimi ve toksisitesi ile ilgili hemen hemen hiç çalışma bulunmamaktadır. Bu çalışmada bromofenol mavisinin biyolojik giderimi ve toksisitesi P. wilhelmii ve Daphnia magna üzerinde test edilmiştir. Maksimum biyolojik giderim 72. saatte %50.04 olarak 5.04 mg/L boya konsantrasyonunda elde edilmiştir. Chl (a+b) konsantrasyonu 0.196 µg/mL olarak kaydedilmiş ve çalışılan en yüksek boya konsantrasyonu olan 76.26 mg/L'de boya giderimi %5.66 olmuştur. Artan bromofenol mavisi konsantrasyonu mikroalg büyümesini tamamen engellememiştir. Bromofenol maruziyetinin D. magna üzerindeki etkileri 24, 48 ve 72. saatlerde incelenmiştir ve akut toksisite etkilerinin maruziyet süresiyle birlikte arttığı görülmüştür. En yüksek akut toksisite etkisi 72. saatte, D. magna için 129.038 mg/L'lik öldürücü bir konsantrasyon olarak belirlenmiştir. Çalışma sonucunda, P. wilhelmii'nin bromofenol mavisi gideriminde kullanılma potansiyeline sahip olduğu ve bu boyanın yüksek konsantrasyonlarda suda yaşayan organizmalar için toksik olabileceği sonucuna varılmıştır.

Keywords

Akut toksisite , Bromofenol mavisi , Daphnia magna , Pseudochloris wilhelmii

Ethical Statement

Bu çalışmanın hazırlanma sürecinde bilimsel ve etik ilkelere uyulduğu ve yararlanılan tüm çalışmaların kaynakçada belirtildiği beyan olunur.

Supporting Institution

TÜBİTAK

Project Number

122Z742

Thanks

Çalışmada kullanılan mikroorganizmalar TÜBİTAK 122Z742 nolu proje kapsamında Burcu ERTİT TAŞTAN tarafından izole edilmiştir.

Bromophenol Blue Removal by Pseudochloris wilhelmii and Acute Toxicity on Daphnia magna

Abstract

The use of dyes is increasing in different industrial areas and this causes environmental problems. Although dyes are removed from wastewater with many different physical and chemical treatment techniques, there is a need for environmentally friendly removal technologies. Although Pseudochloris wilhelmii used in many biotechnological studies, bromophenol blue removal and toxicity have not been investigated yet. Furthermore, there are almost no studies on the bioremoval and toxicity of bromophenol blue dye. In this study bromophenol blue bioremoval and toxicity was tested on P. wilhelmii and Daphnia magna. The maximum bioremoval was obtained at 5.04 mg/L dye concentration as 50.04% at 72 hours. The chl (a+b) concentration was recorded as 0.196 µg/mL and dye removal was 5.66% at the highest dye concentration studied 76.26 mg/L. The increasing bromophenol blue concentration did not completely inhibit the microalgal growth. The effects of bromophenol exposure on D. magna were examined at 24, 48, and 72 hours, and it was observed that the acute toxicity effects increased with exposure time. The highest acute toxicity effect was determined at 72 hours, with a lethal concentration of 129.038 mg/L for D. magna. As a result of the study, it was concluded that P. wilhelmii has the potential to be used for bromophenol blue treatment and this dye may be toxic to aquatic organisms at high concentrations.

Keywords

Acute toxicity , Bromophenol blue , Daphnia magna , Pseudochloris wilhelmii

Ethical Statement

It is declared that scientific and ethical principles were followed during the preparation of this study and that all studies used are stated in the bibliography.

Supporting Institution

TÜBİTAK

Project Number

122Z742

Thanks

The microorganism used in this study was isolated within the scope of TUBITAK Project No 122Z742 of Burcu ERTİT TAŞTAN.

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