Effects of In Vivo / In Vitro Melatonin Application on the Duodenum in Rats with Experimental Hyperthyroidism

Year 2024, Volume: 7 Issue: 5, 210 - 217, 15.09.2024

Esra Şentürk , Fikret Çelebi

https://doi.org/10.19127/bshealthscience.1507825

Abstract

The aim of this study was to investigate the effects of melatonin on the intestinal motility of hyperthyroidism rats. Therefore, we determined in vivo and in vitro effects of melatonin on duodenal tissue in experimental hyperthyroid rats. 34 Wistar-Albino male rats were fed with physiological conditions, and then euthanized by cervical dislocation. The experimental animals, Group 1: Control group (n=5), Group 1B: Melatonin group in vitro (n=5), Group 1C: Melatonin group in vivo (n=6), Group 2: 2A: Hypertension group (n=6), 2B: Group 2: Hyperthyroidi sm in vitro melatonin group (n=6), 2C: Hyperthyroidism in vivo melatonin group (n=6). Acetylcholine (ACh, 10-7, 10-6, 10-5, 10-4, 10-3, 10-2 M), potassium chloride (KCl, 20, 40, 60, 80, 100 mM) at the end of the incubation period different doses were given to the bathing environment. In in vitro melatonin groups, the determined submaximal doses (ACh 10-4 M, KCl 60 mM) and melatonin at different doses (Mel 10-10, 10-9, 10-8, 10-7, 10-6, 10-5 and 10-4 M) were applied. It was determined that the contraction responses of the isolated duodenal tissues induced by KCl and ACh increased significantly (p<0.001) in experimental rats with hyperthyroidism. In the same way, it was found that in the groups treated with melatonin in vivo, there was a significant (P<0.001) increase in the contraction responses compared to those of control group in the isolated tissue. It was found that hyperthyroidism significantly decreased the contraction responses compared with the hyperthyroidism melatonin treated groups in vivo. In melatonin groups, responses to different logarithmic doses of melatonin administered with subcutaneous doses of KCl and ACh were evaluated. According to the findings, contraction responses to different doses of melatonin were found to vary significantly. It was determined that in vivo administration of melatonin on intestinal motility decreased the contraction responses in experimental hyperthyroidism induced rats. Melatonin given in the bath environment in vitro was found to increase or decrease contraction responses on intestinal motility significantly in different doses. Melatonin is thought to be a positive effect of on intestinal motility.

Keywords

Hyperthyroidism, Acetylcholine, Duodenum, KCl, Melatonin, Rat

Project Number

ATA-BAP 2017-6093

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