Effects of In Vivo / In Vitro Melatonin Application on the Duodenum in Rats with Experimental Hyperthyroidism

Yıl 2024, Cilt: 7 Sayı: 5, 210 - 217, 15.09.2024

Esra Şentürk , Fikret Çelebi

https://doi.org/10.19127/bshealthscience.1507825

Öz

The aim of this study was to investigate the effects of melatonin on the intestinal motility of hyperthyroidism rats. Therefore, we determined in vivo and in vitro effects of melatonin on duodenal tissue in experimental hyperthyroid rats. 34 Wistar-Albino male rats were fed with physiological conditions, and then euthanized by cervical dislocation. The experimental animals, Group 1: Control group (n=5), Group 1B: Melatonin group in vitro (n=5), Group 1C: Melatonin group in vivo (n=6), Group 2: 2A: Hypertension group (n=6), 2B: Group 2: Hyperthyroidi sm in vitro melatonin group (n=6), 2C: Hyperthyroidism in vivo melatonin group (n=6). Acetylcholine (ACh, 10-7, 10-6, 10-5, 10-4, 10-3, 10-2 M), potassium chloride (KCl, 20, 40, 60, 80, 100 mM) at the end of the incubation period different doses were given to the bathing environment. In in vitro melatonin groups, the determined submaximal doses (ACh 10-4 M, KCl 60 mM) and melatonin at different doses (Mel 10-10, 10-9, 10-8, 10-7, 10-6, 10-5 and 10-4 M) were applied. It was determined that the contraction responses of the isolated duodenal tissues induced by KCl and ACh increased significantly (p<0.001) in experimental rats with hyperthyroidism. In the same way, it was found that in the groups treated with melatonin in vivo, there was a significant (P<0.001) increase in the contraction responses compared to those of control group in the isolated tissue. It was found that hyperthyroidism significantly decreased the contraction responses compared with the hyperthyroidism melatonin treated groups in vivo. In melatonin groups, responses to different logarithmic doses of melatonin administered with subcutaneous doses of KCl and ACh were evaluated. According to the findings, contraction responses to different doses of melatonin were found to vary significantly. It was determined that in vivo administration of melatonin on intestinal motility decreased the contraction responses in experimental hyperthyroidism induced rats. Melatonin given in the bath environment in vitro was found to increase or decrease contraction responses on intestinal motility significantly in different doses. Melatonin is thought to be a positive effect of on intestinal motility.

Anahtar Kelimeler

Hyperthyroidism, Acetylcholine, Duodenum, KCl, Melatonin, Rat

Proje Numarası

ATA-BAP 2017-6093

Kaynakça

• Aulinas A. 2019. Physiology of the pineal gland and melatonin. Endotext, South Dartmouth, US, URL= https://www.ncbi.nlm.nih.gov/books/NBK550972 (accessed date: February 23, 2024).
• Baltaci AK, Mogulkoc R. 2017. Leptin, NPY, Melatonin and zinc levels in experimental hypothyroidism and hyperthyroidism: The relation to zinc. Biochem Genet, 55: 223-233.
• Bandyopadhyay D, Bandyopadhyay A, Das PK, Reiter RJ. 2002. Melatonin protects against gastric ulceration and increases the efficacy of ranitidine and omeprazole in reducing gastric damage. J Pineal Res, 33: 1-7.
• Bondarenko LA, Sotnik NN, Chagovets EM, Sergienko LY, Cherevko AN. 2011. Intensity of in vitro incorporation of 3H-melatonin in the thyroid gland of rabbits with pineal gland hypofunction. Bull Exper Biol Med, 150: 753-755.
• Brzozowski T, Konturek PC, Konturek SJ, Pajdo R, Bielanski W, Brzozowska I, Stachura J, Hahn EG. 1997. The role of melatonin and L-tryptophan in prevention of acute gastric lesions induced by stress, ethanol, ischemia, and aspirin. J Pineal Res, 23: 79-89.
• Chojnacki C, Walecka-Kapica E, Lokiec K, Pawlowicz M, Winczyk K, Chojnacki J, Klupinska G. 2013. Influence of melatonin on symptoms of irritable bowel syndrome in postmenopausal women. Endokrynologia Polska, 64: 114-120.
• Daher R, Yazbeck T, Jaoude JB, Abboud B. 2009. Consequences of dysthyroidism on the digestive tract and viscera. World J Gastroenterol, 15: 2834-2838.
• Drago F, Macauda S, Salehi S. 2002. Small doses of melatonin increase intestinal motility in rats. Digest Disease Sci, 47: 1969-1974.
• Ganguly K, Maity P, Reiter RJ, Swarnakar S. 2005. Effect of melatonin on secreted and induced matrix metalloproteinase-9 and -2 activity during prevention of indomethacin-induced gastric ulcer. J Pineal Res, 39: 307-315.
• Genç S, Soysal Mİ. 2018. Parametric and nonparametric post hoc tests. BSJ Eng Sci, 1(1): 18-27.
• Hall JE. 2011. Guyton and Hall textbook of medical physiology, 12th ed. Elsevier, Philadelphia, US, pp: 1168.
• Karbownik M, Lewinski A. 2003. The role of oxidative stress in physiological and pathological processes in the thyroid gland; possible involvement in pineal-thyroid interactions. Neuro Endocrinol Lett, 24: 293-303.
• Lee PP, Pang SF. 1993. Melatonin and its receptors in the gastrointestinal tract. Biol Signals, 2: 181-193.
• Lucchelli A, Santagostino-Barbone MG, Tonini M. 1997. Investigation into the contractile response of melatonin in the guinea-pig isolated proximal colon: the role of 5-HT4 and melatonin receptors. British J Pharmacol, 121: 1775-1781.
• Martin MT, Azpiroz F, Malagelada JR. 2005. Melatonin as a modulator of the ileal breake mechanism. Scand J Gastroenter, 40: 559-563.
• Moezi L, Nasiripoor S, Mohajer V, Maghsoodi M, Samini M, Dehpour AR. 2010. Gastric healing effect of melatonin against different gastroinvasive agents in cholestatic rats. Pathhophysiology, 17: 65-70.
• Mogulkoc R, Baltacı AK, Oztekin E, Aydın L, Sivrikaya A. 2006. Melatonin prevents oxidant damage in various tissues of rats with Hyperthroidism. Life Sci, 79: 311-315.
• Pradeepkumar Singh L, Vivek Sharma A, Swarnakar S. 2011. Upregulation of collagenase-1 and -3 in indomethacin- induced gastric ulcer in diabetic rats: role of melatonin. J Pineal Res, 51: 61-74.
• Rom-Bugoslavskaia ES, Bondarenko LA. 1984. Effect of deficiency and excess of thyroid hormones in the body on indolamine metabolism in the rat epiphysis cerebri. Problemy Endokrinol, 30: 82-85.
• Shahid MA, Ashraf MA, Sharma S. 2020. Physiology, thyroid hormone. StatPearls Publishing, Treasure Island, Florida, US, URL= https://www.ncbi.nlm.nih.gov/books/NBK500006 (accessed date: February 21, 2024).
• Sommansson A, Wan Saudi WS, Nylander O, Sjöblom M. 2013. Melatonin inhibits alcohol-induced increases in duodenal mucosal permeability in rats in vivo. Am J Physiol Gastrointestin Liver Physiol, 305: 95-105.
• Sommansson A, Yamskova O, Schiöth HB, Nylander O, Sjöblom M. 2014. Long-term oral melatonin administration reduces ethanol-induced increases in duodenal mucosal permeability and motility in rats. Acta Physiologica, 212: 152-165.
• Touitou Y. 2001. Human aging and melatonin: Clinical relevance. Exp Gerontol, 36: 1083-1100.