EFFECT OF PROPOLIS ON WOUND HEALING: A CLINICAL AND HISTOMORPHOMETRIC STUDY IN RATS

Abstract

Background: Propolis is known to have antioxidant, antiinflammatory, antibacterial, antiviral, immunostimulant and local anesthetic effects. The aim of the present study was to investigate the clinical and histomorphometric effects of propolis on the healing of excisional palatal wounds in rats. Materials and methods: Sixty male Wistar rats were used for the study. Six animals were sacrificed at beginning of the study as initial wound (0 day). The rats were divided into three groups: Propolis (P), Chlorhexidine (CHX) and Control (C). Subjects in all three groups were randomly selected to form nine subgroups of six rats each. Under anesthesia, circular excision wounds with a diameter of 3 mm were formed in the middle of the palate of the rats by punching. The mucoperiosteal part was removed with sharp dissection, and the area on the open bone surface was left to heal the secondary wound. Propolis was administered locally at a rate of 1 ml/day. The rats were sacrificed on days 7, 14, and 21, and pictures of the wound area were taken. Each photograph was transferred to a dedicated program to measure the defect area. Histological sections were taken and the presence of inflammatory cells, epithelialization, and degree of healing were assessed. Results: The average wound area between epithelial margins decreased significantly over time in all groups (p <0.05). compared to CHX and C groups, significant reduction of wound area was observed after seven, 14 and 21 days by using Propolis at (respectively 5.56±3.77; 3.70±1.76; 1.12±0.83) On day 21st day, the inflammatory cells were still observed in the Control group. Conclusion: The results of the study show that Propolis has a positive effect on the healing of soft tissue by accelerating wound healing.

Keywords

• Propolis
• chlorhexidine
• oral wound healing

PROPOLİSİN YARA İYİLEŞMESİNDEKİ ETKİSİ: SIÇANLARDA KLİNİK VE HİSTOMORFOMETRİK BİR ÇALIŞMA

Abstract

Amaç: Propolisin antioksidan, antiinflamatuar, antibakteriyel, antiviral, immunostimulant ve lokal anestezik etkileri olduğu bilinmektedir. Bu çalışmanın amacı, sıçanlarda eksizyonel damak yaralarının iyileşmesinde propolisin klinik ve histomorfometrik etkilerinin araştırılmasıdır. Materyal ve metod: Çalışmada 60 adet erkek Wistar sıçan kullanıldı. Başlangıçta iyileşme referansı olarak (0 gün) altı hayvan sakrifiye edildi, Sıçanlar 3 gruba ayrıldı: Propolis (P), Klorheksidin (CHX) ve Kontrol (K). Tüm gruptaki denekler rastgele seçilerek altışar sıçandan oluşan dokuz alt grup oluşturuldu. Anestezi altına sıçanların damaklarının tam ortasında punch ile 3 mm çapında sirküler eksizyonel yara yüzeyleri oluşturuldu. Mukoperiosteal kısım keskin diseksiyonla uzaklaştırıldı ve açık kemik yüzeyindeki alan sekonder iyileşmeye bırakıldı. Propolis 1 ml/gün olacak şeklide gavaj yoluyla lokal olarak uygulandı. Sıçanlar yedinci, 14. ve 21. günlerde sakrifiye edildi ve yara bölgesinin fotoğrafları çekildi. Her bir fotoğraf özel bir programa aktarılarak defekt bölgesindeki yara alanı ölçüldü. Histolojik kesitler alınarak enflamatuar hücre varlığı, epitelizasyon ve iyileşme düzeylerine bakıldı. Bulgular: Epitelyal marjinler arasındaki ortalama yara alanı, tüm gruplarda zamanla önemli ölçüde azaldı (p <0.05). Propolis grubu, Chx ve K grubu ile karşılaştırıldığında yedi, 14 ve 21. günlerde (sırasıyla 5.56±3.77; 3.70±1.76; 1.12±0.83) belirgin bir yara alanı azalması gözlemlenmiştir 21. günde kontrol grubunda inflamatuar hücreler gözlenmeye devam etti. Sonuç: Çalışmanın bulguları ışığında Propolisin yara iyileşmesini hızlandırarak yumuşak dokunun iyileşmesinde olumlu etkileri gözlenmiştir.

Keywords

• Propolis
• klorheksidin glukonat
• yara iyileşmesi

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