Ligatürle İndüklenen Rat Periodontitis Modelinde Sistemik Darbepoetin Alfa Uygulamasının Alveolar Kemik Kaybı Üzerine Etkisinin Morfometrik Değerlendirilmesi

Abstract

Amaç: Bu deneysel çalışmada, ligatür ile oluşturulan sıçan periodontitis modelinde sistemik darbepoetin alfa uygulamasının alveolar kemik kaybı üzerindeki etkisinin morfometrik yöntemle değerlendirilmesi amaçlandı. Gereç ve Yöntem: Çalışmaya elli erişkin erkek Sprague–Dawley sıçan dahil edildi ve hayvanlar rastgele beş gruba ayrıldı (n=10): Kontrol, Kontrol + darbepoetin alfa (0,25 µg/kg/gün), Periodontitis, Periodontitis + darbepoetin alfa (0,1 µg/kg/gün) ve Periodontitis + darbepoetin alfa (0,25 µg/kg/gün). Deneysel periodontitis, mandibular birinci molar dişlerin çevresine ipek ligatür yerleştirilerek oluşturuldu. Darbepoetin alfa deney süresince 11 gün boyunca intraperitoneal olarak uygulandı. Deney sonunda mandibulalar çıkarıldı ve alveolar kemik kaybı, stereomikroskop altında mine-sement sınırı ile alveolar kemik kreti arasındaki doğrusal mesafenin ölçülmesiyle morfometrik olarak değerlendirildi. İstatistiksel anlamlılık düzeyi p<0,05 olarak kabul edildi. Bulgular: Periodontitis grubunda alveolar kemik kaybı, kontrol gruplarına kıyasla anlamlı derecede yüksekti (p<0,05). Sistemik darbepoetin alfa uygulanan periodontitis gruplarında alveolar kemik kaybı, tedavi uygulanmayan periodontitis grubuna göre anlamlı düzeyde azaldı (p<0,05). Ortalama alveolar kemik kaybı; Kontrol grubunda 0,42 mm, Kontrol + darbepoetin alfa grubunda 0,44 mm, Periodontitis grubunda 1,27 mm, Periodontitis + 0,1 µg/kg/gün darbepoetin alfa grubunda 0,79 mm ve Periodontitis + 0,25 µg/kg/gün darbepoetin alfa grubunda 0,77 mm olarak belirlendi. Darbepoetin alfa uygulanan iki periodontitis grubu arasında alveolar kemik kaybı açısından istatistiksel olarak anlamlı bir fark saptanmadı (p>0,05). Sonuç: Sistemik darbepoetin alfa, ligatür ile oluşturulan deneysel periodontitis modelinde alveolar kemik kaybını anlamlı düzeyde azaltmıştır. Her iki doz benzer koruyucu etki göstermiştir. Bulguların doğrulanması için ileri deneysel ve klinik çalışmalara gereksinim vardır.

Keywords

• Alveolar kemik kaybı
• darbepoetin alfa
• deneysel periodontitis
• morfometrik analiz
• sıçan

Supporting Institution

Eskişehir Osmangazi Üniversitesi Bilimsel Araştırma Projeleri Komisyonu

Project Number

2020-3073

Morphometric Assessment of Alveolar Bone Loss Following Systemic Darbepoetin Alfa Administration in a Rat Model of Ligature-Induced Periodontitis

Abstract

Aim: This experimental study investigated whether systemic administration of darbepoetin alfa could reduce alveolar bone loss in a rat model of ligature-induced periodontitis through morphometric evaluation. Materials and Methods: Fifty adult male Sprague–Dawley rats were randomly allocated to five experimental groups (n = 10): Control, Control receiving darbepoetin alfa (0.25 µg/kg/day), Periodontitis, Periodontitis treated with darbepoetin alfa (0.1 µg/kg/day), and Periodontitis treated with darbepoetin alfa (0.25 µg/kg/day). Experimental periodontitis was established by placing silk ligatures around the mandibular first molars. Darbepoetin alfa was administered intraperitoneally once daily throughout the 11-day experimental period. Following euthanasia, morphometric assessment of alveolar bone loss was performed by determining the linear distance between the cementoenamel junction and the alveolar bone crest using stereomicroscopic images. Results: Ligature placement resulted in a marked increase in alveolar bone loss compared with the healthy control groups (p < 0.05). Administration of darbepoetin alfa significantly limited bone loss in both treatment groups relative to untreated periodontitis (p < 0.05). Mean alveolar bone loss values were 0.42 mm in the Control group, 0.44 mm in the Control + darbepoetin alfa group, 1.27 mm in the Periodontitis group, 0.79 mm in the Periodontitis + 0.1 µg/kg/day group, and 0.77 mm in the Periodontitis + 0.25 µg/kg/day group. No statistically significant difference was identified between the two darbepoetin alfa dosage regimens (p > 0.05). Conclusion: Systemic administration of darbepoetin alfa significantly reduced alveolar bone loss in a ligature-induced rat model of periodontitis. Both treatment doses demonstrated comparable protective effects. Further experimental and clinical studies are needed to confirm these findings.

Keywords

• Alveolar bone loss
• darbepoetin alfa
• experimental periodontitis
• morphometric analysis
• rat

Supporting Institution

Eskişehir Osmangazi Üniversitesi Bilimsel Araştırma Projeleri

Project Number

2020-3073

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