Nano-Amplification Strategy Using Charge-Based Capacitance Measurement for Pathogenic Bacteria Detection

Abstract

Objective: This work will present a nano-amplifi cation strategy for microfabricating Multi-Lab-On-Single-Chip (MLoC) system to enhance the level of
detection and also reduced the number of probe pads for clinical and health care applications, such as pathogenic disease.
Material and
Method:
The Charge-Based Capacitance Measurement (CBCM) technique used Complementary Metal-Oxide-Semiconductor (CMOS) with 350
nm technology, the implementation material is doped silicon. The system employs an interdigitated capacitor structure, charge based
capacitance measurement circuitry to detect and process the variation of the capacitance, which is a function of the permittivity in the
presence of targeted bacteria that is applied to the sensing capacitor but not to the reference one.
Findings and
Results:
The system appeared good sensitivity for low level concentration of bacterial comparing to the literature results. The circuit was measuring
the capacitance changes around 100 aF. The noise at the output of the circuit is around 78.7µV.
Conclusion: CMOS biosensor-based charge-based capacitance measurement system has been fabricated, testing and experimentally validated for
bacterial pathogens cell detection. Three important requirements of such biosensor systems including functionalized sensing capacitance,
passivating the reference capacitance and interface circuit were discussed. The system provides a rapid, low power, and miniaturized
platform that can be mass-produced.

Keywords

• Biosensors
• CBCM
• Escherichia coli
• health applications
• MLoC system
• permittivity

Patojenik Bakteri Algılama İçin Sarj Tabanlı Kapasitans Ölçümü Kullanarak Nano-Amplifikasyon Stratejisi

Abstract

Amaç: Bu çalışma, patojenik hastalık gibi klinik ve sağlık uygulamalarında tespit seviyesini arttırmak ve prob pedlerinin sayısını azaltmak için MultiLab-On-Single-Chip (MLoC) sistemi mikro imalatı için bir nano-amplifi kasyon stratejisi sunmaktadır.
Materyal ve
Metod:
Uygulama malzemesi katkılı silikon, Şarj bazlı kapasitans ölçüm (CBCM) tekniği, 350 nm teknolojisi ile Tamamlayıcı Metal Oksit-Yarıiletken
(CMOS) tekniği kullandı. Algılayıcı kapasitöre uygulanan, ancak referans olana uygulanmayan hedefl enen bakterilerin mevcudiyetinde
oluşan geçirgenliğin sonucu olan, sistem kapasitansın varyasyonunu tespit etmek ve işlemek için interdijite kapasitör yapısı, yüke bağlı
kapasitans ölçüm devresi kullanıldı.
Bulgular: Sistem, literatür sonuçlarına göre düşük seviyede bakteriyel konsantrasyon için iyi bir duyarlılık ortaya koymuştur. Devre, 100 aF civarında
kapasitans değişikliklerini ölçüyordu. Devrenin çıkışındaki gürültü 78.7µV civarındadır.
Tartışma: CMOS biyosensör bazlı şarj bazlı kapasitans ölçüm sistemi, bakteriyel patojenlerin hücre tespiti için test edilmiş ve deneysel olarak
doğrulanmıştır. Fonksiyonel algılama kapasitansı, referans kapasitansı ve ara-yüz devresinin pasifl eştirilmesi de dahil olmak üzere bu tür
biyosensör sistemlerinin üç önemli gerekliliği tartışılmıştır. Sistem seri olarak üretilebilen hızlı, düşük güç ve minyatür bir platform sağlar. 

Keywords

• Escherichia coli
• Biyosensörler
• CBCM
• Escherichia coli
• sağlık uygulamalar
• MLoC sistem
• dielektrik sabiti

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