In Vitro Cytotoxic Effect Of Various Fruits On Human Cervical Carcinoma Cells

Abstract

Objective: The aim of this study was to evaluate the antiproliferative activities of some the fruits, traditionally used in folk medicine. Therefore, the fruits of Fructus cynosbati (Rosehip), Rubus fruticosus (Blackberry), Sorbus torminalis (Wild service tree), Vaccinium myrtillus (Blueberry) and Vitis labrusca (Isabella grape) were used in the present study.

Material and Methods: Methanol extracts of the fruits were obtained and added to ARPE-19 (no-trasformed) and HeLa (transformed) culture media at various concentrations. Taxol and DMSO were used as positive and negative controls, respectively. After 48 h of cell incubation, the cytotoxic effect of the extracts was assessed by MTT. Cell viability values ​​were calculated for each group. The statistical analysis of the data was performed using unpaired t-test. p values ​​less than 0.05 were considered significant.

Findings: V. labrusca showed dose dependent cytotoxic activity against both cell lines. In additions, V. myrtillus and R. fruticosus extracts exhibited moderate cytotoxic effect on both cell lines at higher concentrations. Extracts of F. cynosbati and S. torminalis were found to decrease the cell viability in a dose-dependent manner in HeLa cells and the results were statistically significant (p <0.05). Additionally, S. torminalis extract was found to be the most effective extract against the HeLa cells. 

Conclusion: These results demonsrated that S. torminalis methanol extract has a potent in vitro antiproliferative effect toward the human cervical cancer cell line HeLa. Further work needs to be done on isolation and purification of specific bioactive compounds in S. torminalis methanol extract.

Keywords

• HeLa,Phytotherapie,Sorbus torminalis,MTT

Farklı Meyvelerin İnsan Servikal Kanser Hücreleri Üzerindeki Sitotoksik Etkileri

Abstract

Amaç: Çalışmanın amacı geleneksel olarak halk hekimliğinde kullanılan bazı meyvelerin antiproliferatif aktivitelerini araştırmak olup,  Fructus cynosbati (Kuşburnu), Rubus fruticosus (Böğürtlen), Sorbus torminalis (Akçaağaç yapraklı üvez), Vaccinium myrtillus (Yabanmersini) ve Vitis labrusca (Kokulu üzüm)’ün meyveleri çalışmada kullanılmıştır.

Materyal-Metod: Meyvelerin metanol özütleri elde edilerek, çeşitli konsantrasyonlarda ARPE-19 (normal) ve HeLa (kanser) hücre kültür ortamlarına eklenmiştir. Pozitif ve negatif kontrol olarak sırasıyla, Taxol ve DMSO kullanılmış olup, özütlerin sitotoksik etkisi, 48 saatlik hücre inkübasyon süresinden sonra MTT testi ile değerlendirilmiştir. Verilerin istatistiksel analizi, unpaired t-testi kullanılarak gerçekleştirilmiş olup, p değeri 0.05’ten küçük olan sonuçlar anlamlı olarak kabul edilmiştir.

Bulgular: V. labrusca her iki hücre hattına karşı doza bağlı sitotoksik aktivite göstermiştir.  Ayrıca V. myrtillus ve R. fruticosus, özütlerinin artan konsantrasyonlarda her iki hücre hattında orta derecede sitotoksik etkili olduğu saptanmıştır. Buna ek olarak, F. cynosbati ve S. torminalis özütlerinin sadece HeLa hücrelerinde canlılığı doza bağlı olarak azalttığı görülmüş ve sonuçlar istatistiksel olarak anlamlı bulunmuştur (p <0.05). Fakat çalışmada S. torminalis özütünün HeLa hücrelerine karşı en etkili özüt olduğu saptanmıştır.

Sonuç: Bu sonuçlar, S. torminalis methanol özütünün servikal kanser hücrelerine karşı in vitro koşullarda güçlü antiproliferatif etkisinin olduğunu göstermiştir. Sonuç önemli olup, S. torminalis methanol özütü içindeki spesifik biyoaktif bileşenlerinin izolasyonu ve purifikasyonu için yeni çalışmalara ihtiyaç vardır.

Keywords

• HeLa,Fitoterapi,Sorbus torminalis,MTT

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