Molecular Detection of Human Bocavirus (HBoV) in Stool Samples in Patients with

Abstract

Aim: Acute gastroenteritis is the most important causes of morbidity and mortality, especially in children worldwide. To know the factors that cause gastroenteritis enables effective treatment as well as prevent the use of unnecessary antibiotics. Viral pathogens that cause gastroenteritis; rotavirus (HRV), norovirus (HNoV), sapovirus (HSaV), adenovirus (HAdV), astrovirus (HAstV) and bocavirus (BoV). The aim of study was to investigate the frequency and types of HBOV in patients with gastroenteritis by molecular methods in Aydin province. Material and Method: Between September 2013 and October 2014, a total 96 patients who were admitted to our hospital with stool samples were included. Forty four of patients were female and 52 were male. BoV-DNA was investigated by real-time PCR appropriate to NS-1 gene region. The typing of HBoV was determined by type-specific PCR and the coinfection of the other viruses was determined by multiplex PCR. Results: Two (2 %) of the 96 samples were determined to be HBoV positive. Two samples with positive were determined as HBoV type 1 by type specific real-time PCR. Other types of HBoV not detected. The positive patients for HBoV type 1 were 0-2 years old and male gender. Mixed infection with RV were observed in 2 (100%) patients. Conclusion: It was determined that bocavirus as an etiological agent in patients with acute gastroenteritis may cause gastroenteritis like other frequently detected viruses. The identification of viral agents in gastroenteritis will reduce both the cost of preventing unnecessary antibiotic use and the development of resistance to antibiotics in pathogens.

Keywords

• Human Bocavirus
• gastroenteritis
• stool
• real time PCR

Akut Gastroenteritli Hastalarda İnsan Bokavirüsün Moleküler Yöntem ile Araştırılması

Abstract

Amaç:Akut gastroenteritler tüm dünyada özellikle çocuk yaş grubunda daha fazla olmak üzere morbidite ve mortalitenin en önemli nedenlerinden biridir. Gastroenterite yol açan etkenlerinin bilinmesi, etkin tedavi uygulanmasının yanı sıra gereksiz antibiyotik kullanımının önlenmesini sağlamaktadır. Gastroenterite neden olan viral patojenler; rotavirüs (RV), norovirüs (NoV), sapovirüs (SaV), adenovirüs (HAdV), astrovirüs (HAstV) ve bocavirüs (BoV)’dur. Bu çalışmada Aydın ilinde akut gastroenteritli hastalarda İBoV sıklığının ve tiplerinin moleküler yöntem ile araştırılması amaçlandı. Gereç ve Yöntem: Çalışmaya Eylül 2013 ile Ekim 2014 tarihleri arasında hastanemize başvuran akut gastroenteritli 44 (%46)’ ü kadın, 52 (% 54)’ si erkek olmak üzere toplam 96 hastaya ait dışkı örneği dahil edildi. İnsan BoV-DNA’sı NS-1 gen bölgesine gen bölgesine uygun primer dizilerinin kullanıldığı real-time PZR yöntemi ile araştırıldı. Bokavirüs tiplendirilmesi tipe özgül real time PZR ve koenfeksiyon varlığı multipleks PZR ile belirlendi. Bulgular: Çalışmada incelenen 96 örneğin 2'sinin (% 2) İBoV pozitif olduğu belirlendi. Pozitif iki örnek, tipe özgül gerçek zamanlı PZR ile İBOV tip 1 olarak bulundu. Diğer bokavirüs tipleri saptanmadı. İnsan BoV tip 1 pozitif hastalar erkek cinsiyetinde ve 0-2 yaş grubundaydı. İki hastada (% 100) RV ile koenfeksiyon gözlendi. Sonuç: Bu çalışma ile akut gastroenteritli hastalarda etiyolojik ajan olarak bokavirüsun da diğer sıklıkla saptanan virüsler gibi gastroenterite neden olabileceği belirlenmiştir. Gastroenteritlerde viral etkenlerin belirlenmesi, gereksiz antibiyotik kullanımının önlenmesini hem maliyeti hem de patojenlerde antibiyotiklere karşı direnç gelişimini azaltacaktır.

Keywords

• İnsan Bokavirüs
• gastroenterit
• dışkı
• real-time PZR

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