Molecular Detection of Human Bocavirus (HBoV) in Stool Samples in Patients with

Year 2020, , 256 - 261, 31.12.2020

Fadime Kahyaoğlu , Sevin Kırdar

https://doi.org/10.34084/bshr.773324

Cited By: 1

Abstract

Aim: Acute gastroenteritis is the most important causes of morbidity and mortality, especially in children worldwide. To know the factors that cause gastroenteritis enables effective treatment as well as prevent the use of unnecessary antibiotics. Viral pathogens that cause gastroenteritis; rotavirus (HRV), norovirus (HNoV), sapovirus (HSaV), adenovirus (HAdV), astrovirus (HAstV) and bocavirus (BoV). The aim of study was to investigate the frequency and types of HBOV in patients with gastroenteritis by molecular methods in Aydin province.

Material and Method: Between September 2013 and October 2014, a total 96 patients who were admitted to our hospital with stool samples were included. Forty four of patients were female and 52 were male. BoV-DNA was investigated by real-time PCR appropriate to NS-1 gene region. The typing of HBoV was determined by type-specific PCR and the coinfection of the other viruses was determined by multiplex PCR.

Results: Two (2 %) of the 96 samples were determined to be HBoV positive. Two samples with positive were determined as HBoV type 1 by type specific real-time PCR. Other types of HBoV not detected. The positive patients for HBoV type 1 were 0-2 years old and male gender. Mixed infection with RV were observed in 2 (100%) patients.

Conclusion: It was determined that bocavirus as an etiological agent in patients with acute gastroenteritis may cause gastroenteritis like other frequently detected viruses. The identification of viral agents in gastroenteritis will reduce both the cost of preventing unnecessary antibiotic use and the development of resistance to antibiotics in pathogens.

Keywords

Human Bocavirus, gastroenteritis, stool, real time PCR

Akut Gastroenteritli Hastalarda İnsan Bokavirüsün Moleküler Yöntem ile Araştırılması

Abstract

Amaç:Akut gastroenteritler tüm dünyada özellikle çocuk yaş grubunda daha fazla olmak üzere morbidite ve mortalitenin en önemli nedenlerinden biridir. Gastroenterite yol açan etkenlerinin bilinmesi, etkin tedavi uygulanmasının yanı sıra gereksiz antibiyotik kullanımının önlenmesini sağlamaktadır. Gastroenterite neden olan viral patojenler; rotavirüs (RV), norovirüs (NoV), sapovirüs (SaV), adenovirüs (HAdV), astrovirüs (HAstV) ve bocavirüs (BoV)’dur. Bu çalışmada Aydın ilinde akut gastroenteritli hastalarda İBoV sıklığının ve tiplerinin moleküler yöntem ile araştırılması amaçlandı.

Gereç ve Yöntem: Çalışmaya Eylül 2013 ile Ekim 2014 tarihleri arasında hastanemize başvuran akut gastroenteritli 44 (%46)’ ü kadın, 52 (% 54)’ si erkek olmak üzere toplam 96 hastaya ait dışkı örneği dahil edildi. İnsan BoV-DNA’sı NS-1 gen bölgesine gen bölgesine uygun primer dizilerinin kullanıldığı real-time PZR yöntemi ile araştırıldı. Bokavirüs tiplendirilmesi tipe özgül real time PZR ve koenfeksiyon varlığı multipleks PZR ile belirlendi.

Bulgular: Çalışmada incelenen 96 örneğin 2'sinin (% 2) İBoV pozitif olduğu belirlendi. Pozitif iki örnek, tipe özgül gerçek zamanlı PZR ile İBOV tip 1 olarak bulundu. Diğer bokavirüs tipleri saptanmadı. İnsan BoV tip 1 pozitif hastalar erkek cinsiyetinde ve 0-2 yaş grubundaydı. İki hastada (% 100) RV ile koenfeksiyon gözlendi.

Sonuç: Bu çalışma ile akut gastroenteritli hastalarda etiyolojik ajan olarak bokavirüsun da diğer sıklıkla saptanan virüsler gibi gastroenterite neden olabileceği belirlenmiştir. Gastroenteritlerde viral etkenlerin belirlenmesi, gereksiz antibiyotik kullanımının önlenmesini hem maliyeti hem de patojenlerde antibiyotiklere karşı direnç gelişimini azaltacaktır.

Keywords

İnsan Bokavirüs, gastroenterit, dışkı, real-time PZR

References

• Rikhotso MC, Kabue JP, Ledwaba SE, et al. Prevalence of Human Bocavirus in Africa and Other Developing Countries between 2005 and 2016: A Potential Emerging Viral Pathogen for Diarrhea. J Trop Med 2018, 12: 7875482.
• Özkan A, Çocukluk çağı akut gastroenterit olgularında etiyolojik ajanların belirlenmesi. Uzmanlık tezi. Çukurova Üniversitesi Tıp Fakültesi, Çocuk Sağlığı ve Hastalıkları Anabilim Dalı, Adana, Türkiye-2005.
• Allander T, Tammi MT, Eriksson M, et al. Cloning of a human parvovirus by molecular screening of respiratory tract samples. PNAS USA 2005;102:12891–6.
• Albuquerque MC, Rocha LN, Benati FJ, et al. Human bocavirus infection in children with gastroenteritis, Brazil. Emerg Infect Dis 2007;13(11):1756-8.
• Vicente D, G. Cilla, M. Montes, et al. Human bocavirus, a respiratory and enteric virus. Emerg Infect Dis 2007; 13 (4,): 636-7.
• Arthur Higgins GD, Davidson GP, Givney RC, et al. A novel bocavirus associated with acute gastroenteritis Australian children.PLoSPathogens doi.org/10.1371/journal.ppat.1000391.
• Kapoor A, Simmonds P, Slikas B, et al. Human bocaviruses are highly diverse, dispersed, recombination prone, and prevalent enteric infections. J Infect Dis 2010 1; 201(11): 1633–43.
• Nawaz S, Allen DJ, Aladin F, et al. Human bocaviruses are not significantly associated with gastroenteritis: results of retesting archive DNA from a case control study in the UK. PLoS One 2012;7 :e41346 doi: 10.1371/journal.pone.0041346.
• Mitui MT, Bozdayi G, Ahmed S, et al. Detection and molecular characterization of diarrhea causing viruses in single and mixed infections in children: a comparative study between Bangladesh and Turkey. J Med Virol 2014 Jul;86(7):1159-68.
• La Rosa G, Della Libera S, Laconelli M, et al. Human bocavirus in children with acutegastroenteritis in Albania. J Med Virol 2016; 88(5): 906-10.
• Lee JI, Chung JY, Han TH, et al. Detection of human bocavirus in children hospitalized because of acute gastroenteritis. J Infect Dis, 2007; 196(7): 994-7.
• Lau SK, Yip CC, Que TL, et al. Clinical and molecular epidemiology of human bocavirus in respiratory and fecal samples from children in Hong Kong. J Infect Dis 2007; 196: 986-93.
• Chow BD, Ou Z, Esper FP. Newly recognized bocaviruses (HBoV, HBoV2) in children and adults with gastrointestinal illness in the United States. J Clin Virol 2010; 47: 143-47.
• Schildgen O. Human Bocavirus: Lessons learned to date; Pathogens 2013, 2: 1-12.
• Campos GS, Sampaio MLS, Menezes ADL, et al. Human Bocavirus in Acute Gastroenteritis in Children in Brazil. J Med Virol 2016; 88:166–70.
• Romani S, Mohebbi SR, Khanyaghma M, et al. Detection of Human Bocavirus 1, 2 and 3 from patients with acute gastroenteritis. Gastroenterology Hepatology from Bed to Bench 2013; 6:77–81.
• Kim S. Prevalence of human bocavirus 1 among people without gastroenteritis symptoms in South Korea between 2008 and 2010. Arch Virol 2014;159 (10):2741-4.
• Soares LS, Lima AB, Pantoja KC, et al. Detection and Molecular Epidemiology of Human Bocavirus in Children with Acute Gastroenteritis from Brazil. BioRxiv doi: https://doi.org/10.1101/404632.
• Martínez MA, Soto-Del Río Mde L, et al. DNA microarray for detection of gastrointestinal viruses. J Clin Microbiol 2015; 53: 136-45.
• Rovida F, Campanini G, Piralla A, et al. Molecular de¬tection of gastrointestinal viral infections in hospitalized patients. Diagn Microbiol Infect Dis 2013; 77: 231-5.
• Zhou T, Chen Y, Chen J, et al. Prevalence and Clinical profile of human bocavirus in children with acute gastroenteritis in Chengdu, West China, 2012-2013. J Med Virol 2017; 89(10):1743-48.
• Midilli K, Yılmaz G, Türkoğlu S, ve ark. Akut solunum yolu enfeksiyonlu çocuk ve erişkinlerde insan bokavirus DNA’sının polimeraz zincir reaksiyonu ile saptanması. Mikrobiyol Bul, 2010; 44: 405-13.
• Uyar M, Kuyucu N, Tezcan S, ve ark. Bronşiyolit Tanısı Alan 0-2 Yaş Grubu Çocuklarda İnsan Bokavirus ve Diğer Solunum Viruslarının Sıklığının Araştırılması. Mikrobiyol Bul 2014; 48(2):242-58.
• Sirekbasan S, Midilli K, Akın Y, ve ark. Akut gastroenteritli çocuklarda human Bocavirus DNA varlığının araştırılması. Turk Hij Den Biyol Derg 2017;74(4): 261-68.
• Alam MM, Khurshid A, Shaukat S, et al. Hu¬man bocavirus in Pakistani children with gastroenteritis. J Med Virol 2015; 87: 656-63.
• Wang Y, Gonzalez R, Zhou H, et al. Detection of human bocavirus 3 in China. European Journal of Clinical Microbiology & Infectious Diseases 2011;30,799–805.
• Zhang DM, Ma MM, Wen WT, et al. Clinical epidemiology and molecular profiling of human bocavirus in faecal samples from children with diarrhoea in Guangzhou, China. Epidemiol Infec 2014;3:1-15.
• Tymentsev A, Tikunov A, Zhirakovskaia E, et al. Human bocavirus in hospitalized children with acute gastroenteritis in Russia from 2010 to 2012. Infect Genet Evol 2016;37:143-9.
• Lee EJ, Kim HS, Kim HS, et al. Human Bocavirus in Korean Children with Gastroenteritis and Respiratory Tract Infections. BioMed Res Inter 2016;5.
• Netshikweta R, Chidamba L, Nadan S, Taylor MB, Page N.Molecular epidemiology of human bocavirus infection in hospitalized children with acute gastroenteritis in South Africa, 2009‐2015. J Med Virol. 2020;92:1124–1132.
• Proenca-Modena JL, Martinez M, Amarilla AA, et al. Viral load of human bocavirus-1 in stools from children with viral diarrhoea in Paraguay. Epidemiol Infect 2013 ;141(12):2576-80.