Lemuru Fish Oil (Sardinella longiceps) Therapy on Periodontal Wistar Rats Induced with Porphyromonas gingivalis Bacteria: Osteoblast and Osteoclast

Year 2019, Volume: 3 Issue: 2, 128 - 134, 29.08.2019

Dian Widya Damaiyanti , Widyastuti Widyastuti Ayulistya Paramita Aditya Megantara Mohammad Ibrohim

https://doi.org/10.34084/bshr.597830

Cited By: 2

Abstract

Background: Periodontitis is an
inflammatory disease of tooth-supporting tissue, caused by the bacterium such
as Porphyromonas gingivalis. Inflammation that not treated can lead to
resorption of alveolar bone. It is suggested that supportive medicines are
needed to regenerate bone, one of them is lemuru fish oil gel (Sardinella longiceps). Lemuru fish oil can act as an immunomodulator and anti-bacterium.
Flavonoid content in fish oil plays a role to inhibit lipid peroxidation so it
is effective in killing bacteria that can reduce LPS production. The content of
eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in lemuru fish oil
lead to modulate IL-10, inhibition the production of pro-inflammatory cytokines
such as interleukin-1 beta (IL-1 β), prostaglandin-E2 (PGE2), tumor necrosis
factor alfa (TNFα) that will decrease receptor activator of nuclear factor
kappa-Β - ligand (RANK-RANKL) band and result is increased osteoblast,
decreased osteoclasts number which speeds up the bone regeneration process Objective: To determine the effect of
topically applying lemuru fish oil in on periodontal Wistar Rats after
induction of Periodontitis with P.gingivalis bacteria.

Materials and methods: Experimental research
laboratories with post-test only control group design. Samples of 25 male
Wistar rats divided into 5 groups. K- (no induction), K (induced with P.
gingivalis), P1 (induced with P. gingivalis and 10% lemuru oil gel therapy), P2
(induced with P. gingivalis and 20% lemuru fish oil gel therapy), P3 (induced
with  P. gingivalis and 40% lemuru oil
gel therapy). Induction of the bacterium P.gingivalis was carried out, then the
lemuru fish oil gel was given to gingival sulcus for 14 days. Observations were
made by calculating osteoblast and osteoclast cells in histology samples using
HE (Hematoxylin Eosin).

Results: The LSD statistic
test showed a significant difference between the P. gingivalis induction group
with no treatment and treatment group using lemuru fish oil. The treatment
using lemuru fish oil gel can increase the number of osteoblasts and decrease
the number of osteoclast to the induction of P.gingivalis bacteria, with the
most effective number of osteoblasts found in P2 group with a concentration of
20% lemuru fish oil gel therapy.

Conclusion: Topical Lemuru Fish
oil gel can be used as alveolar bone regeneration therapy on periodontitis
induced with P.gingivalis.

Keywords

Topical lemuru fish oil gel, Osteoblasts, Osteoclast, Periodontitis, Phorphyromonas gingivalis

Wistar Sıçanlarında Porphyromonas gingivalis Bakteri ile Elde Edilen Periodontal hastalıkta Lemuru Balık Yağı (Sardinella longiceps) ile Tedavi: Osteoblast ve Osteoclast

Abstract

Amaç: Periodontitis, Porphyromonas
gingivalis gibi bakterilerin neden olduğu enflamatuar bir dişi destekleyen
dokular hastalığıdır. Tedavi edilmeyen iltihap alveoler kemiğin emilmesine
neden olabilir. Kemiğin yenilenmesi için destekleyici ilaçlara ihtiyaç
duyulduğu önerilmektedir, bunlardan biri lemuru balık yağı jelidir (Sardinella longiceps). Lemuru balık yağı, bir immünomodülatör ve anti-bakteriyel etki
gösterebilir. Balık yağındaki flavonoid içeriği, lipit peroksidasyonunu inhibe
etmede rol oynar, bu nedenle LPS üretimini azaltabilecek bakterilerin
öldürülmesinde etkilidir. Lemuru balık yağındaki eikosapentaenoik asit (EPA) ve
dokosahekssaenoik asitin (DHA) içeriği, interlökin-1 beta (IL-1 β),
prostaglandin-E2 (IL-1 β), prostaglandin-E2 (IL-1 β) gibi pro-enflamatuar
sitokinlerin üretimini inhibe eder. PGE2), nükleer faktör kappa-Β - ligand
(RANK-RANKL) bandının reseptör aktivatörünü azaltacak olan tümör nekroz faktörü
alfa (TNFa) ve sonuçta osteoblast artar, kemik rejenerasyon işlemini
hızlandıran osteoklast sayısını düşürür. Çalışmada topikal lemuru balık
yağının, Wistarlarda P.gingivalis periodontiti tedavisine etkisinin
belirlenmesi amaçlanmıştır.

Materal ve Metod: Test ve
kontrol grubu için deneysel araştırma laboratuvarlarında 25 erkek Wistar ratı 5
gruba ayrıldı. K- (indüksiyon yok), K (P.gingivalis ile indüklenmiş), P1
(P.gingivalis ve% 10 lemuru yağ jel terapisi ile indüklenmiş), P2 (P.gingivalis
ve% 20 lemuru balık yağı jel terapisi ile indüklenmiş), P3 (P.gingivalis ve% 40
lemuru yağ jeli tedavisi ile indüklenmiştir). Bakterinin indüksiyonu
P.gingivalis yapıldı ve daha sonra lemuru balık yağı jeli, 14 gün boyunca
gingival sulkusa sürüldü. Hematoksilen Eosin(HE) boyası ile histoloji
örneklerinde osteoblast ve osteoklast hücreleri hesaplanarak gözlemler yapıldı.

Bulgular: LSD istatistik testi lemuru balık
yağı kullanılarak tedavi ve tedavi grubu olmayan P.gingivalis indüksiyon grubu
arasında anlamlı bir fark gösterdi. Lemuru balık yağı jeli kullanılarak yapılan
muamele, osteoblast sayısını artırabilir ve P2 grubunda% 20 lemuru balık yağı
jeli terapisi en etkili osteoblast sayısı vermiş ve osteoklast sayısını
azaltabildiği gösterilmiştir.

Sonuç: Topikal Lemuru Balık yağı jeli,
P.gingivalis ile indüklenen periodontitlerde alveoler kemik rejenerasyon
tedavisi olarak kullanılabilir.

Keywords

Topikal lemuru balık yağı jeli, Osteoblast, Osteoklast, Periodontitis, Periodontitis, Porphyromonas gingivalis

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