Kakule Ekstraktının Adjuvan Kombinasyonu ile Meme Kanseri Hücre Hattı Üzerinde İmmünostimülan/Sitotoksik Etkisinin İncelenmesi
Abstract
Meme kanseri, kadınlar arasında en sık görülen kanser türüdür ve kansere bağlı ölümlerin en sık ikinci nedenidir. Meme dokusundaki hücrelerin çeşitli faktörler sonucu kontrolsüz çoğalmasından kaynaklanır. Meme kanseri tedavisinde genel olarak kimyasal ilaçlar kullanılmaktadır. Ancak kimyasal kanser ilaçları sağlıklı hücreler üzerinde sitotoksik etkiye sahiptir. Bu, doz sınırlamasına ve tedavinin etkinliğinin azalmasına neden olur. Bu nedenle meme kanseri tedavisinde bitkisel temelli çalışmalar gün geçtikçe popüler hale gelmektedir. Literatürde birçok bitkinin antikanser, antienflamatuar ve antimikrobiyal özellikleri analiz edilmiş ve etkinlikleri kanıtlanmıştır. Elettaria Cardamomum (E. Cardamomum, Cardamom) bitkisinde bulunan diindolilmetan (DIM) ve indol-3-karbinol (I3C) molekülleri metastazı, tümör oluşumunu, immünomodülasyonu azaltır. E. Cardamomum'un meme kanseri hücresinde apoptotik yolakları indükleyerek meme kanseri gelişimini önlemede rol oynadıkları literatürde gösterilmiştir. Burada, THP-1 insan makrofaj hücre hattına ve MCF-7 meme kanseri hücre hattına kakule ekstraktı ve kakule ekstrakt-Freund'un adjuvan kombinasyonu uygulanarak meme kanseri gelişiminin önlenmesi ve kakule ekstraktının etkinliğinin adjuvan yardımıyla artırılması amaçlanmaktadır. Sonuç olarak kakule Ekstrakt-Freund adjuvan kombinasyonunun meme kanseri hücreleri üzerindeki sitotoksik etkisinin tek başına kakule ekstraktı uygulamasına göre daha yüksek olduğu belirlendi. Aynı zamanda, bitki ektraktı konsantrasyonunun arttırılmasıyla Freund's adjuvanının toksik etkisinin azaltılabileceği belirlendi.
References
- J. A. Kareparamban, P. H. Nikam, A. Jadhav, and V. J. Kadam, "Ferula foetida “ Hing ” : A Review," 2012.
- G. N. Sharma, R. Dave, J. Sanadya, P. Sharma, and K. K. Sharma, "Various types and management of breast cancer: an overview," Journal of advanced pharmaceutical technology & research, vol. 1, no. 2, pp. 109-126, 2010.
- N. Bhagat and A. Chaturvedi, "Spices as an Alternative Therapy for Cancer Treatment," Systematic Reviews in Pharmacy, vol. 7, pp. 46-56, 2016.
- B. B. Aggarwal and H. Ichikawa, "Molecular targets and anticancer potential of indole-3-carbinol and its derivatives," Cell Cycle, vol. 4, no. 9, pp. 1201-15, Sep. 2005.
- J. R. Weng, C. H. Tsai, S. K. Kulp, and C. S. Chen, "Indole-3-carbinol as a chemopreventive and anti-cancer agent," Cancer Lett, vol. 262, no. 2, pp. 153-63, Apr. 2008.
- H. F. Stills, Jr., "Adjuvants and antibody production: dispelling the myths associated with Freund's complete and other adjuvants," Ilar j, vol. 46, no. 3, pp. 280-93, 2005.
- B. Marongiu, A. Piras, and S. Porcedda, "Comparative analysis of the oil and supercritical CO2 extract of Elettaria cardamomum (L.) Maton," J Agric Food Chem, vol. 52, no. 20, pp. 6278-82, Oct. 2004.
- M. Ihlamur, B. Akgül and E. S. Abamor., ''Farklı hücre hatlarında besiyeri ve FBS’in hücre proliferasyonu üzerindeki etkilerinin incelenmesi,'' Süleyman Demirel Üniversitesi Fen Edebiyat Fakültesi Fen Dergisi vol. 17, pp. 55-64, 2022.
- I. Guevara, "Determination of nitrite/nitrate in human biological material by the simple Griess reaction," Clin Chim Acta, vol. 274, no. 2, pp. 177-88, Jun 22 1998.
- J. van Meerloo, G. J. Kaspers, and J. Cloos, "Cell sensitivity assays: the MTT assay," Methods Mol Biol, vol. 731, pp. 237-45, 2011.
- G. M. Cragg and J. M. Pezzuto, "Natural Products as a Vital Source for the Discovery of Cancer Chemotherapeutic and Chemopreventive Agents," Medical principles and practice : international journal of the Kuwait University, Health Science Centre, vol. 25 Suppl 2, no. Suppl 2, pp. 41-59, 2016.
- N. Vutakuri and S. Somara, "Natural and herbal medicine for breast cancer using Elettaria cardamomum (L.) Maton," International Journal of Herbal Medicine, vol. 6, pp. 91-96, 2018.
- E. Solowey, M. Lichtenstein, S. Sallon, H. Paavilainen, E. Solowey, and H. Lorberboum-Galski, "Evaluating medicinal plants for anticancer activity," Scientific World Journal, vol. 2014, p. 721402, 2014.
- J. Oscherwitz, F. C. Hankenson, F. Yu, and K. B. Cease, "Low-dose intraperitoneal Freund's adjuvant: toxicity and immunogenicity in mice using an immunogen targeting amyloid-beta peptide," Vaccine, vol. 24, no. 15, pp. 3018-25, Apr 5 2006.
- H. Manaheji, S. Jafari, J. Zaringhalam, S. Rezazadeh, and R. Taghizadfarid, "Analgesic effects of methanolic extracts of the leaf or root of Moringa oleifera on complete Freund's adjuvant-induced arthritis in rats," Zhong Xi Yi Jie He Xue Bao, vol. 9, no. 2, pp. 216-22, Feb 2011.
Immunostimulant/Cytotoxic Effect of Cardamom Extract with Adjuvant Combination on Breast Cancer Cell Line
Abstract
Breast cancer is the most common type of cancer among women and is the second most common cause of cancer-related deaths. It is caused by the uncontrolled proliferation of cells in the breast tissue as a result of various factors. For the treatment of breast cancer, chemical drugs are utilized generally. However, chemical cancer drugs have a cytotoxic effect on healthy cells. That causes dose limitation and a decrease in the effectiveness of the treatment. For this reason, herbal-based studies in the treatment of breast cancer become popular day by day. In the literature, the anticancer, anti-inflammatory, and antimicrobial properties of many plants have been analyzed and their effectiveness has been proven. Diindolylmethane (DIM) and indole-3-carbinol (I3C) molecules found in Elettaria Cardamomum (E. Cardamomum, Cardamom) plant, reduce metastasis, tumorigenesis, immunomodulation. It has been shown in the literature that they play a role in preventing the development of breast cancer via inducing apoptotic pathways in the breast cancer cell. Herein, it is aimed to prevent breast cancer development by applying cardamom extract and cardamom Extract-Freund's adjuvant combination to THP-1 human macrophage cell line and MCF-7 breast cancer cell line and to increase the efficiency of cardamom extract with the use of an adjuvant. As a result, it was determined that the cytotoxic effect of the cardamom Extract-Freund’s adjuvant combination on breast cancer cells was higher than the alone cardamom extract application. At the same time, it was determined that the toxic effect of Freund's adjuvant could be reduced by increasing the plant extract concentration.
References
- J. A. Kareparamban, P. H. Nikam, A. Jadhav, and V. J. Kadam, "Ferula foetida “ Hing ” : A Review," 2012.
- G. N. Sharma, R. Dave, J. Sanadya, P. Sharma, and K. K. Sharma, "Various types and management of breast cancer: an overview," Journal of advanced pharmaceutical technology & research, vol. 1, no. 2, pp. 109-126, 2010.
- N. Bhagat and A. Chaturvedi, "Spices as an Alternative Therapy for Cancer Treatment," Systematic Reviews in Pharmacy, vol. 7, pp. 46-56, 2016.
- B. B. Aggarwal and H. Ichikawa, "Molecular targets and anticancer potential of indole-3-carbinol and its derivatives," Cell Cycle, vol. 4, no. 9, pp. 1201-15, Sep. 2005.
- J. R. Weng, C. H. Tsai, S. K. Kulp, and C. S. Chen, "Indole-3-carbinol as a chemopreventive and anti-cancer agent," Cancer Lett, vol. 262, no. 2, pp. 153-63, Apr. 2008.
- H. F. Stills, Jr., "Adjuvants and antibody production: dispelling the myths associated with Freund's complete and other adjuvants," Ilar j, vol. 46, no. 3, pp. 280-93, 2005.
- B. Marongiu, A. Piras, and S. Porcedda, "Comparative analysis of the oil and supercritical CO2 extract of Elettaria cardamomum (L.) Maton," J Agric Food Chem, vol. 52, no. 20, pp. 6278-82, Oct. 2004.
- M. Ihlamur, B. Akgül and E. S. Abamor., ''Farklı hücre hatlarında besiyeri ve FBS’in hücre proliferasyonu üzerindeki etkilerinin incelenmesi,'' Süleyman Demirel Üniversitesi Fen Edebiyat Fakültesi Fen Dergisi vol. 17, pp. 55-64, 2022.
- I. Guevara, "Determination of nitrite/nitrate in human biological material by the simple Griess reaction," Clin Chim Acta, vol. 274, no. 2, pp. 177-88, Jun 22 1998.
- J. van Meerloo, G. J. Kaspers, and J. Cloos, "Cell sensitivity assays: the MTT assay," Methods Mol Biol, vol. 731, pp. 237-45, 2011.
- G. M. Cragg and J. M. Pezzuto, "Natural Products as a Vital Source for the Discovery of Cancer Chemotherapeutic and Chemopreventive Agents," Medical principles and practice : international journal of the Kuwait University, Health Science Centre, vol. 25 Suppl 2, no. Suppl 2, pp. 41-59, 2016.
- N. Vutakuri and S. Somara, "Natural and herbal medicine for breast cancer using Elettaria cardamomum (L.) Maton," International Journal of Herbal Medicine, vol. 6, pp. 91-96, 2018.
- E. Solowey, M. Lichtenstein, S. Sallon, H. Paavilainen, E. Solowey, and H. Lorberboum-Galski, "Evaluating medicinal plants for anticancer activity," Scientific World Journal, vol. 2014, p. 721402, 2014.
- J. Oscherwitz, F. C. Hankenson, F. Yu, and K. B. Cease, "Low-dose intraperitoneal Freund's adjuvant: toxicity and immunogenicity in mice using an immunogen targeting amyloid-beta peptide," Vaccine, vol. 24, no. 15, pp. 3018-25, Apr 5 2006.
- H. Manaheji, S. Jafari, J. Zaringhalam, S. Rezazadeh, and R. Taghizadfarid, "Analgesic effects of methanolic extracts of the leaf or root of Moringa oleifera on complete Freund's adjuvant-induced arthritis in rats," Zhong Xi Yi Jie He Xue Bao, vol. 9, no. 2, pp. 216-22, Feb 2011.
Details
| Primary Language | English |
|---|---|
| Subjects | Engineering |
| Journal Section | Research Articles |
| Authors | |
| Early Pub Date | December 28, 2022 |
| Publication Date | December 31, 2022 |
| Published in Issue | Year 2022 Volume: 5 Issue: 2 |
