Akut Enflamasyonlu Tavşanlarda Kortistatin Uygulamasının Antioksidan Sistem ve Sitokin Seviyeleri Üzerine Etkileri

Yıl 2017, Cilt: 7 Sayı: 2/2, 190 - 201, 28.12.2017

Banu Atalay

Öz

Çalışmada,
beyinden izole edilen kortistatin (CST) isimli endojen bir nöropeptidin,
terebentin yağı ile akut enflamasyon oluşturulan tavşanlarda antioksidan sistem
parametreleri ve sitokin düzeyleri üzerine etkilerinin incelenmesi
amaçlanmıştır. Sağlıklı, 28 adet erkek Yeni Zelanda ırkı tavşan kullanılan
araştırmada, hayvanlar kontrol (C), terebentin uygulaması (T), kortistatin
uygulaması (250 µg/kg) (CST) ve terebentin + kortistatin (250 µg/kg) (T+CST)
uygulaması olacak şekilde rastgele dört gruba ayrılmıştır. 2.saatte ve 6.saatte
alınan kan örneklerinden antioksidan ve oksidan sistemlere ait indirgenmiş
glutatyon (GSH), süper oksit dismutaz (SOD) ve malondialdehit (MDA)
düzeylerinin, enflamatuar sitokinlerden ise interlöykin-6 (IL-6) ve tümör
nekrozis faktör -α (TNF-α) seviyelerinin incelendiği araştırmada kortistatin
uygulaması, T+K grubunda GSH seviyesinde T grubuna nazaran anlamlı derecede
koruyucu seviyenin sürdürülmesine katkıda bulunurken (p<0.05), enflamasyonla
birlikte dokuda lipid peroksidasyon düzeyinin artışına bağlı olarak yükselen
MDA seviyesi ve SOD seviyeleri açısından ise istatistiki olarak önemli olmayan
bir değişikliğe yol açmıştır (p>0.05). Çalışmanın sonucunda elde edilen
verilere dayanarak kortistatin, enflamasyonu baskılayıcı bir rol oynadığına
dair etkiler gözlenmesine rağmen, çalışma dizaynı kısa sürdüğü için, bunun
endotoksemi ve sepsis gibi durumlarda gözlenen etkisinden küçük olduğu sonucuna
varılmıştır.

Anahtar Kelimeler

kortistatin, antioksidan parametreler, redükte glutatyon, malondialdehit, interlöykin-6

The Effects of Cortistatin Administration on Plasma Antioxidant System and Cytokine Levels of Rabbits with Acute Inflammation

Öz

In the study, it was aimed to research the effects of an endogenous
neuropeptide called cortistatin (CST) which is isolated from the brain on
parameters of antioxidant system and cytokine levels of rabbits with turpentine
oil induced acute inflammation. In the study using 28 healthy, male rabbits
from New Zealand breed, animals were randomly divided into four groups
consisting of control (C), turpentine (T), cortistatin (250 µg/kg) (CST), and
turpentine + cortistatin (250 µg/kg) (T+CST). Reduced glutathione (GSH), super
oxide dismutase (SOD, malondialdehyde (MDA) levels belonging to antioxidant and
oxidant systems, interleukin-6 (IL-6) and tumor necrosis factor -α (TNF-α)
levels from inflammatory cytokines were studied in the blood samples taken
after 2 and 6 hours and while cortistatin administration significantly
contributed for maintaining GSH at protective level in T+CST group (p<0.05),
it caused a change which was not statistically significant with respect to MDA
and SOD levels increasing based on elevated lipid peroxidation levels in the
tissue along with the inflammation (p>0.05). Depending on resulting data of
the study, it was concluded that despite there were effects observed regarding
that cortistatin plays an inflammation suppressing role, this was smaller than
the effect observed in conditions such as endotoxemia and sepsis because design
of study did not lasted for long.

Anahtar Kelimeler

cortistatin, antioxidant parameters, reduced glutathione, malondialdehyde, interleukin-6

Kaynakça

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