Qualitative Analysis of Alfalfa Seed Methanol Extract by GC-MS and Determination of Antioxidant Properties

Abstract

Alfalfa plant has a great importance for agriculture in Turkey and throughout the world. Antioxidant activities (DPPH and NO scavenging effects) have been studied from the methanol extraction of the seeds, and organic volatile molecule contents have been analyzed by GC-MS. Physical and chemical analysis of the seed were also carried out to elucidate the structure. As a result of the experiments, a concentration-dependent increase was observed in DPPH and NO scavenging (%) activities from the 0.5, 1 and 2 mg/ml methanol extract concentrations of alfalfa seeds. In GC-MS analysis, it was found that squalene, a pharmacologically active molecule, is present in alfalfa seeds. As a result of chemical and physical analyzes, it was determined that the crude protein content is 33.79% and crude oil is 8.11%. Although alfalfa is widely used in agriculture and as animal bait, this study shows that alfalfa seeds are also pharmacologically crucial for containing rich molecules

Keywords

• squalene,Clover,DPPH,NO,scavenging activity

References

1. 1. Bagavathiannan, MV, Van, ARC. 2009. The Biology and Ecology of Feral Alfalfa (Medicago sativa L.) and its implications for Novel Trait Confinement in North America. Critical Reviews in Plant Sciences; 28(1): 69-87.
2. 2. Bora, KS, Sharma, A. 2011. Evaluation of Antioxidant and Cerebroprotective Effect of Medicago sativa Linn. against Ischemia and Reperfusion Insult. Evidence-Based Complementary and Alternative Medicine; 2011: 1-9.
3. 3. Musa, EM, EL-Badwi, SM, Jah Elnabi, MA, Osman, EA, Dahab, MM. 2012. Hepatoprotective and toxicity assessment of Cannabis sativa seed oil in albino rat. International Journal of Chemical and Biochemical Sciences; 1: 69-76.
4. 4. Younas, J, Hussain, F. 2014. In vitro Antidiabetic Evaluation of Allium sativum L. International Journal of Chemical and Biochemical Sciences; 5: 22-25.
5. 5. Gaweł, E, Grzelak, M, Janyszek, M. 2017. Lucerne (Medicago sativa L.) in the human diet-case reports and short reports. Journal of Herbal Medicine; 10: 8-16.
6. 6. TÜİK. 2016. Türkiye İstatistik Kurumu. http://www.tuik.gov.tr.
7. 7. Abdallah, EM, Khalid, AE. 2012. A preliminary evaluation of the antibacterial effects of Commiphora molmol and Boswellia papyrifera oleo-gum resins vapor. International Journal of Chemical and Biochemical Sciences; 1: 1-5.
8. 8. Hamid, AA, Aiyelaagbe, OO. 2012. Pharmacological investigation of Asystasia calyciana for its antibacterial and antifungal properties. International Journal of Chemical and Biochemical Sciences; 1: 99-104.

9. 9. Hanif, MA, Al-Maskari, AY, Al-Sabahi, JN, Al-Hdhrami, I, Khan, MM, Al-Azkawi, A, Hussain, AI. 2015. Chemical characterisation of bioactive compounds in Medicago sativa growing in the desert of Oman. Natural Product Research; 29(24): 2332-2335.
10. 10. Gray, JD, McEwen, BS. 2013. Lithium's role in neural plasticity and its implication for mood disorders. Acta Psychiatrica Scandinavica; 128(5): 347-361.
11. 11. Karimi, E, Oskoueian, E, Oskoueian, A, Omidvar, V, Hendra, R, Nazeran, H. 2013. Insight into the functional and medicinal properties of Medicago sativa (alfalfa) leaves extract. Journal of Medicinal Plants Research; 7(7): 290-297.
12. 12. Rafinska, K, Pomastowski, P, Wrona, O, Górecki, R, Buszewskbi, B. 2017. Medicago sativa as a source of secondary metabolites for agriculture and pharmaceutical industry. Phytochemistry Letters; 20: 520-539.
13. 13. Zhang, GW, He, L, Hu, MM. 2011. optimized ultrasonic-assisted extraction of flavonoids from Prunella vulgaris L. and evaluation of antioxidant activities in vitro. Innovative Food Science & Emerging; 12(1): 18-25.
14. 14. Marcocci, I, Marguire, JJ, Droy-Lefaiz, MT, Packer, L. 1994. Thenitricoxide scavenging propertyof Ginkgo biloba extract. Biochemical and Biophysical Research Communications; 201: 748-755.
15. 15. Xie, Z, Huang, J, Xu, X, Jin, Z. 2008. Antioxidant activity of peptides isolated from alfalfa leaf protein hydrolysate. Food Chemistry; 111: 370-376.
16. 16. Do, R, Kiss, RS, Gaudet, D, Engert, JC. 2009. Squalene synthase: a critical enzyme in the cholesterol biosynthesis pathway. Clinical Genetics; 75: 19-29.
17. 17. Goldstein, JL, DeBose-Boyd, RA, Brown, MS. 2006. Protein sensors for membrane sterols. Cell; 124: 35-46.
18. 18. Jahaniaval, F, Kakuda, Y, Marcone, M.2000. Fatty acid and triacylglycerol compositions of seed oils of five Amaranthus accessions and their comparison to other oils. Journal of the American Oil Chemists' Society; 77: 847-852.
19. 19. Vogler, U, Rott, AS, Gessler, C, Dorn, S. 2009. Terpene-mediated parasitoid host location behavior on transgenic and classically bred apple genotypes. Journal of Agricultural and Food Chemistry; 57: 6630-6635.
20. 20. Apostol, L, Iorga, S, Mosoiu, C, Racovita, RC, Niculae, OM, Vlasceanu, G. 2017. Alfalfa concentrate a rich source of nutrients for use in food products. Journal of International Scientific Publications; 5: 66-73.