THE EFFECTS OF GLUCOCORTICOIDS ON THE DISTRIBUTION OF FIBRONECTIN, CADHERIN AND BETA-CATENIN MOLECULES DURING MOUSE LUNG DEVELOPMENT

Year 2009, Volume: 5 Issue: 1, 27 - 38, 01.03.2009

Pervin Toparlak Mesut Şahin Ayça Gümüş Erdal Balcan

Abstract

Mammalian lung development involves complex relationships between cells and extracellular matrices. This process mediated by a series of matrix components and adhesion molecules such as fibronectin, laminin, cadherin, catenin and collagen. Glucocorticoids play a central role in fetal lung development. For example it is known that, glucocorticoid administration during pregnancy alters the maturation of the lung. In the present study, we used an immunohistochemical method to evaluate the possible effect of exogenous glucocorticoids to developmental profile of fibronectin, cadherin and betacatenin molecules. Dexamethasone was administrated to pregnant mice on days 11, 12, 13 and 14 of gestation. Animals were sacrificed on days 12, 13, 14 and 15, and stained with polyclonal antibodies to fibronectin, pan-cadherin and beta-catenin. Our results indicated that staining reactivities of fibronectin, pancadherin and beta-catenin were similar in distal epithelia, conductive airways, basal membranes, luminal surfaces of airways and mesenchymal cells. Therefore, we suggest that, 24 hourly antenatal dexamethasone treatments couldn’t completely affect fibronectin, cadherin and beta-catenin contents in evaluated periods of developing lung.

Keywords

Lung development, Dexamethasone, Fibronectin, Cadherin, Beta-catenin, Mouse

FARE AKCİĞER GELİŞİMİNDE GLUKOKORTİKOİDLERİN FİBRONEKTİN, KADERİN VE BETA-KATENİN MOLEKÜLLERİNİN DAĞILIŞLARI ÜZERİNE ETKİSİ

Abstract

Memeli akciger gelisimi hücreler ile ekstrasellüler matriksler arasında gerçeklesen kompleks etkilesimleri içerir. Bu süreç, fibronektin, laminin, kaderin, katenin ve kollajen gibi bir dizi matriks bileseni ve adezyon molekülü tarafından düzenlenmektedir. Glukokortikoidler fötal akciger gelisiminde merkezi bir role sahiptir. Örnegin, gebelik sırasında glukokortikoid uygulamasının akciger gelisiminde degisiklige neden oldugu bilinmektedir. Bu çalısmada, eksojen glukokortikoidlerin fibronektin, kaderin ve beta-katenin moleküllerinin gelisimsel profili üzerine etkilerini belirlemek için immunohistokimyasal bir yöntem uygulandı. Deksametazon gebeligin 11., 12., 13. ve 14. günlerinde hamile farelere verildi. Hayvanlar 12., 13., 14. ve 15. günlerde kesildi ve fibronektin, pan-kaderin ve beta-katenin poliklonal antikorları ile boyandı. Bulgularımız, distal epitelyum, iletici hava kanalları, bazal membranlar, kanalların lümene bakan yüzeyleri ve mezensim hücrelerinde fibronektin, pan-kaderin ve beta-katenin reaktivitelerinin benzer oldugunu gösterdi. Bu sonuçlar, 24 saatlik dogum öncesi deksametazon uygulamalarının incelenen devrelerde gelisen akciger dokularında fibronektin, kaderin ve beta-katenin içerikleri üzerine tam bir etkisinin olmadıgını düsündürmüstür.

Keywords

Akciğer gelişimi, Deksametazon, Fibronektin, Kaderin, Beta-katenin, Fare

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• Geliş Tarihi: 20/01/2009 Kabul Tarihi: 09/04/2009