A mini report on palynological and antibacterial tests of four propolis samples from different regional origins

Year 2020, Volume: 16 Issue: 2, 143 - 148, 24.06.2020

Sinan Bayram , Fatma Necmiye Kacı Nesrin Ecem Bayram , Arzu Görmez

Abstract

In this study, palynological and antibacterial tests of propolis samples from Iran-Turan (IP1 and IP2), Mediterranean (MP), and Europe-Siberia (EP) phytogeographical regions were performed. The pollens of Asteraceae, Betulaceae, Boraginaceae, Brassicaceae, Campanulaceae, Caryophyllaceae, Fabaceae, Fagacee, Lamiaceae, Pinaceae, Salicaceae, Ranunculaceae, Rosaceae and Scrophulariaceae taxa were found in the palynological analysis. However, gram negative and gram positive bacteria were used to determine in vitro antibacterial activities of the propolis samples. The most potent inhibitory effect against the target microorganisms was obtained from IP1. The most resistant strains were Burkholderia cepacia, Citrobacter freundii and Streptococcus pneumoniae for all the propolis samples. But, the antibacterial activity levels of the samples were quite different from each other. These results indicate that propolis can be assessed in different areas such as cosmetic, medicine and food as an antimicrobial agent.

Keywords

antibacterial, minimum inhibition concentration, palynology, pollen analysis

References

• [1]. Sorkun, K., Süer, B., & Salih, B. (2001). Determination of chemical composition of Turkish propolis. Zeitschrift für Naturforschung C, 56(7-8), 666-668.
• [2]. Socha R, Gałkowska D, Bugaj M, Juszczak L. 2015. Phenolic composition and antioxidant activity of propolis from various regions of Poland. Natural Product Research, 29:416-422.
• [3]. Sanpa S, Popova M, Tunkasiri T, Eitssayeam S, Bankova V, Chantawannakul P. 2017. Chemical profiles and antimicrobial activities of Thai propolis collected from Apis mellifera. Chiang Mai Journal of Science, 44: 438-448.
• [4]. Popova M, Reyes M, Le Conte Y, Bankova V.2014. Propolis chemical composition and honeybee resistance against Varroa destructor. Natural Product Research, 28, 788-794.
• [5]. Ahangari Z, Naseri M, Vatandoost F. 2018. Propolis: chemical composition and its applications in endodontics. Iranian Endodontic Journal, 13: 285-292.
• [6]. Al-Waili N. 2018. Mixing two different propolis samples potentiates their antimicrobial activity and wound healing property: a novel approach in wound healing and infection. Veterinary World, 11:1188-1195.
• [7]. Kalthoff S, Strassburg CP. 2019. Contribution of human udp glucuronosyltransferases to the antioxidant effects of propolis, artichoke and silymarin. Phytomedicine, 56:35-39.
• [8]. Ribeiro M, da Luz C, de Albuquerque P. 2019. Palynology as a tool for distinguishing geopropolis samples from stingless bee species in the Maranhense Amazon, Brazil. J. Apic. Res., 58:16-36.
• [9]. Bauer AW, Kirby MDK, Sherries JC. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 1966; 45:493–6.
• [10]. Bayram S, Bayram NE, Gercek YC, Aydogan MN, Oz GC. 2017. Chemical analysis and antimicrobial effect of propolis from Hakkari province of Turkey against some pathogenic microorganisms. European Journal of Biology, 76:74-78.
• [11]. Warakomska Z, Maciejewicz W. 1992. Microscopic analysis of propolis from Polish regions. Apidologie, 23:277-283.
• [12]. Sorkun K, Türkiye'nin nektarlı bitkileri, polenleri ve balları. Palme Yayıncılık, 2012.
• [13]. https://www.paldat.org/
• [14]. Rahman MM, Richardson A, Sofian-Azirun M. 2010. Antibacterial activity of propolis and honey against Staphylococcus aureus and Escherichia coli. African Journal of Microbiology Research, 4:1872-1878. [15]. Ristivojević P, Dimkić I, Trifković J, Berić T, Vovk I, Milojković-Opsenica D, Stanković S. 2016. Antimicrobial activity of Serbian propolis evaluated by means of MIC, HPTLC, bioautography and chemometrics. PloS One, 11:1-15.
• [16]. Grange JM, Davey RW. 1990. Antibacterial properties of propolis (bee glue). Journal of the Royal Society of Medicine, 83(3): 159-160.
• [17]. Morgado LN, Lima DD, Mendonça CBF, Gonçalves-Esteves V. 2018. Pollen spectrum in propolis of Tetragonisca Angustula Latreille (Apidae: Trigonini) in an Island Area, Brazil. Bee World, 95: 84-88.
• [18]. Kızılpınar Temizer İ, Güder A, Gençay Çelemli Ö. 2017. Botanical origin and antioxidant activities of propolis from the Irano-Turanian region. Istanbul Journal of Pharmacy 47 (3): 107-111.
• [19]. Gençay Ö, Sorkun K. 2006. Microscopic analysis of propolis samples collected from east anatolia (Kemaliye-Erzincan). FABAD Journal of Pharmaceutical Sciences, 31: 192-197.
• [20]. Ecem Bayram N, Sorkun K, Cevahir Oz G. 2018. Plant Origins of Propolis from Hakkari, Turkey. Gazi University Journal of Science, 31 (3):725-738
• [21]. Almeida-Muradian L, Pamplona LC, Coimbra Sl, Barth OM. 2005. Chemical composition and botanical evaluation of dried bee pollen pellets. Journal of Food Composition and Analysis, 18: 105-111.
• [22]. Ramalho, M., Kleinert-GiovanninI, A., & Imperatriz-Fonseca, V. L. (1990). Important bee plants for stingless bees (Melipona and Trigonini) and Africanized honey bees (Apis mellifera) in habitats: a review. Apidologie, 21, 469–488.
• [23]. Escriche I, Juan-Borrás M. 2018. Standardizing the analysis of phenolic profile in propolis. Food Research International, 106:834-841.
• [24]. Pratami DK, Mun’im A, Yohda M, Hermansyah H, Gozan M, Putri YRP, Sahlan M. Total phenolic content and antioxidant activity of spray-dried microcapsules propolis from Tetragonula species. In AIP 2019. Conference Proceedings (Vol. 2085, No. 1, p. 020040). AIP Publishing.
• [25]. Cushnie TT, Lamb AJ. 2005. Antimicrobial activity of flavonoids. International Journal of Antimicrobial Agents, 26:343-356.
• [26]. Bankova V, Marcucci M. 2000. Standardization of propolis: present status and perspectives. Bee World, 81:182-188.
• [27]. Pobiega K, Krasniewska K, Gniewosz M. 2019. Application of propolis in antimicrobial and antioxidative protection of food quality - A Review. Trends in Food Science & Technology, 83 53-62.
• [28]. Goren N, Woerdenbag HJ, Bozok-Johansson C. 1996. Cytotoxic and antibacterial activity of sesquiterpene lactones isolated from Tenacetum praeteritum sub sp. praeteritum. Planta Medica, 62:419-422.
• [29]. Niño J, Narváez DM, Mosquera OM, Correa YM. 2006. Antibacterial, antifungal and cytotoxic activities of eight Asteraceae and Two Rubiaceae plants from Colombian Biodiversity. Brazilian Journal of Microbiology. 37: 566-570.
• [30]. AL-Ani I, Zimmermann S, Reichling J, Wink M. 2018. Antimicrobial activities of European propolis collected from various geographic origins alone and in combination with antibiotics. Medicines, 5(1), 1-17.