Resveratrol Supplementation is Associated with Elemental Metabolism in Some Tissues of Acute Swimming Rats

Abstract

The aim of this study was to investigate how resveratrol administration affects element metabolism in some tissues in rats undergoing acute swimming exercise. In the study, 28 adult male Wistar rats were used and the animals were divided into 4 groups in equal numbers. Group 1, Control; Group 2, Swimming: The group fed a standard diet and had 30 minutes of acute swimming exercise. Group 3, Resveratrol: The group receiving 10 mg/kg resveratrol supplement for four weeks. Group 4, Resveratrol + Swimming: The group that received 10 mg/kg resveratrol supplement for four weeks and also had 30 minutes of acute swimming exercise. At the end of the four-weeks diet, group 2 and group 4 animals were given 30 minutes of acute swimming exercise. Animals were sacrificed immediately after swimming practices, and testis, kidney, heart and liver tissue samples were taken. Elemental analyzes in tissue samples were determined by atomic emission method. Elements measured in testicular tissue were not affected by exercise and resveratrol applications. Both resveratrol administration and acute swimming exercise resulted in changes in zinc, iron, magnesium, selenium, molybdenum levels in kidney tissue, iron and calcium levels in heart tissue, and zinc, iron and molybdenum levels in liver tissue (p<0.05). The results of study, which revealed that resveratrol administration caused changes in element metabolism in some tissues of rats both during and independently of exercise, can be presented as the first and original finding based on med-line scans.

Keywords

• Acute swimming exercise
• Element metabolism
• Resveratrol application
• Rat

Resveratrol Desteği Akut Yüzme Egzersizi Yaptırılan Sıçanların Bazı Dokularında Element Metabolizmasıyla İlişkilidir

Abstract

Bu çalışmanın amacı, akut yüzme egzersizi yaptırılan sıçanlarda resveratrol uygulamasının bazı dokulardaki element metabolizmasını nasıl etkilediğini araştırmaktır. Wistar cinsi 28 adet erişkin erkek sıçan kullanılan araştırmada hayvanlar eşit sayıda 4 gruba ayrıldı. Grup 1, Kontrol; Grup 2, Yüzme: Standart diyetle beslenen ve 30 dakika akut yüzme egzersizi yaptırılan grup. Grup 3, Resveratrol: Dört hafta boyunca 10 mg/kg resveratrol desteği alan grup. Grup 4, Resveratrol + Yüzme: Dört hafta boyunca 10 mg/kg resveratrol desteği alan ve 30 dakika akut yüzme egzersizi yaptırılan grup. Dört haftalık diyet uygulamalarının bitiminde grup 2 ve grup 4 hayvanlarına 30 dakika akut yüzme egzersizi yaptırıldı. Yüzme uygulamalarından hemen sonra hayvanlar sakrifiye edilerek testis, böbrek, kalp ve karaciğer doku örnekleri alındı. Alınan doku örneklerinde element analizleri atomik emisyon yöntemiyle tayin edildi. Testis dokusundaki ölçümü yapılan elementler egzersiz ve resveratrol uygulamalarından etkilenmedi. Hem resveratrol uygulaması hem de akut yüzme egzersizi böbrek dokusunda çinko, demir, magnezyum, selenyum, molibden, kalp dokusunda demir, kalsiyum ve karaciğer dokusunda ise çinko, demir, molibden seviyelerinde değişikle sonuçlandı (p<0.05). Resveratrol uygulamasının hem egzersizde, hem de egzersizden bağımsız olarak sıçanların bazı dokularında element metabolizmasında değişikliklere yol açtığını ortaya koyan çalışmamızın bulguları med-line taramalardan yola çıkarak ilk ve orijinal bulgu olarak takdim edilebilir.

Keywords

• Akut yüzme egzersizi
• Element metabolizması
• Resveratrol uygulaması
• Rat

References

1. Baltaci, S.B., Mogulkoc, R., & Baltaci, A.K. (2016). Resveratrol and exercise. Biomedical Reports, 5(5),525-530. https://doi.org/10.3892/br.2016.777
2. Bicer, M., Akil, M., Baltaci, A.K., Mogulkoc, R., Sivrikaya, A., & Akkus, H. (2015). Effect of melatonin on element distribution in the liver tissue of diabetic rats subjected to forced exercise. Bratislavské Lekárske Listy, 116(2), 119-123. https://doi.org/10.4149/bll_2015_023
3. Bobrowska-Korczak, B., Skrajnowska, D., & Tokarz, A. (2012). The effect of dietary zinc--and polyphenols intake on DMBA-induced mammary tumorigenesis in rats. Journal of Biomedical Science, 9(1), 43. https://doi.org/10.1186/1423-0127-19-43
4. Córdova, A., & Navas, F.J. (1998). Effect of training on zinc metabolism: Changes in serum and sweat zinc concentrations in sportsmen.Annals of Nutrition & Metabolism, 42(5), 274-282. https://doi.org/10.1159/000012744
5. Cordova, A. (1994). Zinc content in selected tissues in streptozotocin-diabetic rats after maximal exercise. Biological Trace Element Research, 42(3), 209-216. https://doi.org/10.1007/BF02911518
6. Fischer, P.W., Giroux, A., & L'Abbé, M.R. (1984) Effect of zinc supplementation on copper status in adult man. American Journal of Clinical Nutrition, 40(4),743-746. https://doi.org/10.1093/ajcn/40.4.743
7. Ghio, A.J., Soukup, J.M., Ghio, C., Gordon, C.J., Richards, J.E., Schladweiler, M.C., Snow, S.J., & Kodavanti, U.P. (2021) Iron and zinc homeostases in female rats with physically active and sedentary lifestyles. Biometals, 34(1), 97-105. https://doi.org/10.1007/s10534-020-00266-w
8. Heffernan, S.M., Horner, K., De Vito, G., & Conway, G.E. (2019) The role of mineral and trace element supplementation in exercise and athletic performance: A Systematic review. Nutrients,11(3), 696. https://doi.org/10.3390/nu11030696

9. Jo, E., Bartosh, R., Auslander, A.T., Directo, D., Osmond, A., &Wong, M.W. (2019). Post Exercise recovery following 30-day supplementation of trans-resveratrol and polyphenol-enriched extracts. Sports (Basel), 7(10), 226. https://doi.org/10.3390/sports7100226
10. Kaptanoğlu, B., Turgut, G., Genç, O., Enli, Y., Karabulut, I., Zencir, M., & Turgut, S. (2003). Effects of acute exercise on the levels of iron, magnesium, and uric acid in liver and spleen tissues. Biological Trace Element Research, 91(2), 173-178. https://doi.org/10.1385/BTER:91:2:173
11. Kaya, O., Gokdemir, K., Kilic, M., & Baltaci, A.K. (2006). Melatonin supplementation to rats subjected to acute swimming exercise: Its effect on plasma lactate levels and relation with zinc. Neuro Endocrinology Letters, 27(1-2), 263-266.
12. Kharb, S., & Singh, V. (2000). Magnesium deficiency potentiates free radical production associated with myocardial infarction. The Journal of the Association of Physicians of India, 48(5), 484-485. Retrieved from: http://www.japi.org/
13. Kuru, O., Sentürk, U.K., Gündüz, F., Aktekin, B., & Aktekin, M.R. (2003). Effect of long-term swimming exercise on zinc, magnesium, and copper distribution in aged rats. Biological Trace Element Research, 93(1-3), 105-112. https://doi.org/10.1385/BTER:93:1-3:105
14. Liu, Y.Q., Duan, X.L., Chang, Y.Z., Wang, H.T., & Qian, Z.M. (2006). Molecular analysis of increased iron status in moderately exercised rats. Molecular and Cellular Biochemistry, 282(1-2), 117-123. https://doi.org/10.1007/s11010006-1522-4
15. Lukaski, H.C. (1989). Effects of exercise training on human copper and zinc nutriture. Advances in Experimental Medicine and Biology, 258, 163-170. https://doi.org/10.1007/978-1-4613-0537-8_14
16. Maughan, R.J. (1999). Role of micronutrients in sport and physical activity. British Medical Bulletin, 55(3), 683-690. https://doi.org/10.1258/0007142991902556
17. McDonald, R., Hegenauer, J., & Saltman, P. (1986) Age-related differences in the bone mineralization pattern of rats following exercise. Journal of Gerontology,41(4), 445-452. https://doi.org/10.1093/geronj/41.4.445
18. Mercken, E.M., Carboneau, B.A., Krzysik-Walker, S.M., & de Cabo, R. (2012). Of mice and men: the benefits of caloric restriction, exercise, and mimetics. Aging Research Reviews, 11(3), 390-398. https://doi.org/10.1016/j.arr.2011.11.005
19. Navas, F.J., &Córdova, A. (2000). Iron distribution in different tissues in rats following exercise. Biological Trace Element Research, 73(3), 259-268. https://doi.org/10.1385/BTER:73:3:259
20. NIST Standard reference materials catalog. (1996). NIST Special Publication 260, Standards Reference Material Programme, U.S. Department of Commerce, Gaithersburg, MD 20899-0001, USA
21. Ozturk, A., Baltaci, A.K., Mogulkoc, R., Oztekin, E., Sivrikaya, A., Kurtoglu, E., & Kul, A. (2003). Effects of zinc deficiency and supplementation on malondialdehyde and glutathione levels in blood and tissues of rats performing swimming exercise. Biological Trace Element Research,94(2), 157-166. https://doi.org/10.1385/BTER:94:2:157
22. Patlar, S., Gulnar, U., Baltaci, A.K., & Mogulkoc, R. (2014) Effect of nocturnal exhaustion exercise on the metabolism of selected elements. Archives of Biological Sciences, 66(4),1595-1601. https://doi.org/10.2298/ABS1404595P
23. Pingitore, A., Lima, G.P., Mastorci, F., Quinones, A., Iervasi, G., & Vassalle, C. (2015). Exercise and oxidative stress: potential effects of antioxidant dietary strategies in sports. Nutrition, 31(7-8), 916-922. https://doi.org/10.1016/j.nut.2015.02.005
24. Schrauwen, P., & Timmers, S. (2014). Can resveratrol help to maintain metabolic health? The Proceedings of the Nutrition Society, 73(2), 271-277. https://doi.org/10.1017/S0029665113003856
25. Sharman, J.E., La Gerche, A., & Coombes, J.S. (2015). Exercise and cardiovascular risk in patients with hypertension. American Journal of Hypertension, 28(2), 147-158. https://doi.org/10.1093/ajh/hpu191
26. Sivrikaya, A., Akil, M., Bicer, M., Kilic, M., Baltaci, A.K., & Mogulkoc, R. (2013). The effect of selenium supplementation on elements distribution in liver of rats subject to strenuous swimming. Bratislavské Lekárske Listy,114(1), 12-14. https://doi.org/10.4149/bll_2013_003
27. Sivrikaya, A., Bicer, M., Akil, M., Baltaci, A.K., & Mogulkoc, R. (2012). Effects of zinc supplementation on the element distribution in kidney tissue of diabetic rats subjected to acute swimming. Biological Trace Element Research,147(1-3), 195-199. https://doi.org/10.1007/s12011-011-9284-6
28. Skrajnowska, D., Bobrowska-Korczak, B., Tokarz, A., Bialek, S., Jezierska, E., & Makowska, J. (2013). Copper and resveratrol attenuates serum catalase, glutathione peroxidase, and element values in rats with DMBA-induced mammary carcinogenesis. Biological Trace Element Research,156(1-3), 271-278. https://doi.org/10.1007/s12011013-9854-x
29. Wells, I.C. (2008). Evidence that the etiology of the syndrome containing type 2 diabetes mellitus results from abnormal magnesium metabolism. Canadian Journal of Physiology and Pharmacology, 86(1-2), 16-24. https://doi.org/10.1139/y07-122
30. Zhao, L., Wang, Y., Wang, Z., Xu, Z., Zhang Q., & Yin, M. (2015) Effects of dietary resveratrol on excess-iron-induced bone loss via antioxidative character. The Journal of Nutritional Biochemistry, 26(11), 1174-1182. https://doi.org/10.1016/j.jnutbio.2015.05.009